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vector pqtev psmd10  (Addgene inc)
93
Addgene inc vector pqtev psmd10
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Vector Pqtev Psmd10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/pmc12649946-61-8-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
vector pqtev psmd10 - by Bioz Stars, 2026-08
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pqtev psmd10 bacterial expression vector  (Addgene inc)
93
Addgene inc pqtev psmd10 bacterial expression vector
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Pqtev Psmd10 Bacterial Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/pm35758870-536-1-8?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pqtev psmd10 bacterial expression vector - by Bioz Stars, 2026-08
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recombinant lc3 pqtev map1lc3a vectors  (Addgene inc)
91
Addgene inc recombinant lc3 pqtev map1lc3a vectors
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Recombinant Lc3 Pqtev Map1lc3a Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/pmc08179393__SC___012___D0SC03603J___s001-9-4-21?v=Addgene+inc
Average 91 stars, based on 1 article reviews
recombinant lc3 pqtev map1lc3a vectors - by Bioz Stars, 2026-08
91/100 stars
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pqtev bacterial expression vector  (Addgene inc)
90
Addgene inc pqtev bacterial expression vector
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Pqtev Bacterial Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/pmc07542843-77-12-21?v=Addgene+inc
Average 90 stars, based on 1 article reviews
pqtev bacterial expression vector - by Bioz Stars, 2026-08
90/100 stars
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pqtev vector  (Bio Basic Canada)
90
Bio Basic Canada pqtev vector
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Pqtev Vector, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/10__3762_slash_bjoc__13__115-82-15-18?v=Bio+Basic+Canada
Average 90 stars, based on 1 article reviews
pqtev vector - by Bioz Stars, 2026-08
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pqtev prdx2 vector expressing hprxii  (Addgene inc)
88
Addgene inc pqtev prdx2 vector expressing hprxii
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Pqtev Prdx2 Vector Expressing Hprxii, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/pmc03755041-318-1-8?v=Addgene+inc
Average 88 stars, based on 1 article reviews
pqtev prdx2 vector expressing hprxii - by Bioz Stars, 2026-08
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pqtev vector  (New England Biolabs)
99
New England Biolabs pqtev vector
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Pqtev Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/pm24180436__cb400681n_si_001-91-13-20?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
pqtev vector - by Bioz Stars, 2026-08
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pqtev vector  (Entelechon GmbH)
90
Entelechon GmbH pqtev vector
Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length <t>PSMD10</t> gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).
Pqtev Vector, supplied by Entelechon GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pqtev+vector/pm22101605-257-9-11?v=Entelechon+GmbH
Average 90 stars, based on 1 article reviews
pqtev vector - by Bioz Stars, 2026-08
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Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length PSMD10 gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).

Journal: Biomolecules

Article Title: A Rapid, High-Throughput Method for the Construction of Mutagenesis Libraries

doi: 10.3390/biom15111511

Figure Lengend Snippet: Library design and intermediate plasmid construction strategy. ( A ) Schematic of oligonucleotide library design. The full-length PSMD10 gene was divided into 10 sublibraries (P1–P10). Taking sublibrary P4 as an example, each sublibrary’s oligonucleotides contain 19 bp constant primer-binding regions (blue segments) at both ends, enabling specific amplification of the corresponding variable region. The central region is the variable segment (yellow), where each position is systematically mutated to an amber stop codon (TAG). Each sublibrary’s oligonucleotide pool consists of 24 unique sequences that share identical primer-binding regions and carry a single TAG mutation at a specific site within the variable region. ( B ) Intermediate plasmid assembly strategy. Using sublibrary P4 as an example, the PSMD10 gene was divided into constant and variable regions, with the variable region corresponding to each sublibrary. The intermediate plasmid backbone contains predefined BsmBI restriction sites flanking the vacant variable region. During sublibrary construction, all other regions remain unchanged, and only the corresponding variable region sequences are synthesized and inserted into the intermediate plasmid via BsmBI-mediated assembly (indicated by thick black arrows).

Article Snippet: During the construction of the original plasmid, the vector pQTEV- PSMD10 (#31332, Addgene, Cambridge, MA, USA) was used as the template to amplify the target sequence using the following primers: EF1α-PSMD10-F: 5′-AGGTAGCTGATATCGAATTCCTGGGGATCCACCGGTCCACGGGGGTGTGTCTAAC-3′ and EF1α-PSMD10-R: 5′-AGGTCAGGCTCGCTGAATTCCCCAATGTCAAGC-3′.

Techniques: Plasmid Preparation, Binding Assay, Amplification, Mutagenesis, Synthesized