Review



anti γ ppar  (Proteintech)


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    Structured Review

    Proteintech anti γ ppar
    Anti γ Ppar, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 715 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppar+%CE%B3/PPAR+gamma+Polyclonal+antibody/pm42903678-211-35-39
    Average 96 stars, based on 715 article reviews
    anti γ ppar - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Elevated asprosin expression exacerbates synovial inflammation by PPAR-γ-dependent mechanisms in rheumatoid arthritis.
    Article Snippet: Asprosin, a recently identified adipokine involved in metabolic and inflammatory processes.. The case-control study was conducted to explore how asprosin is involved in the pathogenesis of rheumatoid arthritis (RA).. 205 RA patients and 205 healthy controls were included, and MH7A cells were used for in vitro studies.

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
    Article Snippet: .. After blocking with the blocking solution at room temperature 20 min, sections were incubated at 4 °C overnight with primary antibodies against FXR (1:500, Immunoway, YN2161), MMP-2 (1:500, Immunoway, YT2798), MMP-9 (1:500, Immunoway, YT1892), PPAR-γ (1:500, Proteintech, 16643-1-AP), NLRP3 (1:500, Immunoway, YT5382), IL-6 (1:500, WL, WL02841), IL-18 (1:500, WL, WL01127), and TNF-α (1:500, WL, WL01896). ..

    Incubation:

    Article Title: Elevated asprosin expression exacerbates synovial inflammation by PPAR-γ-dependent mechanisms in rheumatoid arthritis.
    Article Snippet: Asprosin, a recently identified adipokine involved in metabolic and inflammatory processes.. The case-control study was conducted to explore how asprosin is involved in the pathogenesis of rheumatoid arthritis (RA).. 205 RA patients and 205 healthy controls were included, and MH7A cells were used for in vitro studies.

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
    Article Snippet: .. Tissue sections of each group were blocked with 1 % bovine serum albumin and 10 % donkey serum at room temperature for 1 h and then incubated at 4 °C overnight with primary antibodies for neuronal nuclear protein (NeuN) (1:500, Abcam, ab104224), ionized calcium-binding adapter molecule 1 (IBA-1) (1:500, Abcam, ab5076), glial fibrillary acidic protein (GFAP) (1:500, Millipore, MAB360), FXR (1:200, Proteintech, 25055–1-AP), MMP-2 (1:200, Proteintech, 10373-2-AP), MMP-9 (1:200, Proteintech, 10375-2-AP), PPAR-γ (1:200, Proteintech, 16643–1-AP), NLRP3 (1:200, Immunoway, YT5382), and cluster of differentiation 86 (CD86) (1:200, CST, #91882). ..

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
    Article Snippet: Protein samples were separated using 7.5–12.5 % sodium dodecyl sulfate–polyacrylamide gel electrophoresis before being transferred to polyvinylidene fluoride membranes (Millipore Corp., Bedford, MA, USA). .. Membranes were blocked using 5 % skim milk at room temperature for 1 h and subsequently incubated at 4 °C overnight with primary antibodies against FXR (1:1000, Immunoway, YN2161), zonula occludens-1 (ZO-1) (1:1000, Affinity, AF5145), Mucin-2 (1:1000, Abcam, ab272692), occludin (1:1000, Proteintech, 27260–1-AP), claudin-1 (1:1000, WL, WL03073), MMP-2 (1:1000, Immunoway, YT2798), MMP-9 (1:1000, Immunoway, YT1892), PPAR-γ (1:1000, Proteintech, 16643-1-AP), NOD-like receptor protein-3 (NLRP3) (1:1000, Immunoway, YT5382), interleukin-6 (IL-6) (1:1000, WL, WL02841), IL-18 (1:1000, WL, WL01127), tumor necrosis factor-alpha (TNF-α) (1:1000, WL, WL01581), IL-10 (1:1000, WL, WL03088), FGF15 (1:500, Santacruz, sc-514647), TGR5 (1:1000, Abcam, ab72608), BSEP (1:1000, Abcam, ab155421), CYP7α1 (1:1000, Abcam, ab65596) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, 60004-1-Ig). .. Following a 1-hour incubation with secondary antibodies, signals were detected using an enhanced chemiluminescence (ECL) western blotting detection system, and images were captured and analyzed using a chemiluminescence imaging system (Bio-Rad, USA).

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
    Article Snippet: .. After blocking with the blocking solution at room temperature 20 min, sections were incubated at 4 °C overnight with primary antibodies against FXR (1:500, Immunoway, YN2161), MMP-2 (1:500, Immunoway, YT2798), MMP-9 (1:500, Immunoway, YT1892), PPAR-γ (1:500, Proteintech, 16643-1-AP), NLRP3 (1:500, Immunoway, YT5382), IL-6 (1:500, WL, WL02841), IL-18 (1:500, WL, WL01127), and TNF-α (1:500, WL, WL01896). ..

    Article Title: A stage specific NETs-related signature in alcoholic steatohepatitis: from molecular subtyping to therapeutic vulnerabilities
    Article Snippet: .. Membranes were blocked and incubated overnight at 4 °C with primary antibodies against FOS (1:2000, Abclonal, A24620), MMP7 (1:1000, Proteintech, 10374-2-AP), CXCL6 (1:1000, Abclonal, TD13470), PPAR-α (1:500, Bioss, bs-23398R), PPAR-γ (1:1000, Proteintech, 16643-1-AP), HO-1 (1:1000, Abmart, PY5393S), MDA (1:1000, abcam, ab27642), Arg1 (1:5000, Proteintech, 16001-1-AP), MCP-1 (1:500, CST, 2029S), IL-10 (1:1000, Proteintech, 82191-3-RR) and β-actin (1:10,000, Proteintech, 20536-1-AP). .. Following incubation with HRP-conjugated secondary antibodies (1:8,000, Proteintech, SA00001-2), protein bands were visualized using a LI-COR imaging system.

    Article Title: Paratenon mimetic multifunctional bifacial hydrogel for postoperative Achilles tendon remodeling
    Article Snippet: Achilles tendon rupture remains one of the most challenging musculoskeletal injuries to restore functionally.. Although the paratenon—a natural sheath surrounding the tendon—plays a critical role in orchestrating tendon remodeling by balancing intrinsic (tenocyte-mediated) and extrinsic (inflammatory and fibroblast-driven) healing processes, its functional regeneration after injury or surgery remains a significant clinical hurdle.. Here, we report a paratenon-mimetic bifacial hydrogel (PMBH) that reinstates this balance through a bioinspired, multifunctional design (integrating anti-adhesion, antioxidative, and inflammation-modulating properties).

    Tissue:

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
    Article Snippet: .. Tissue sections of each group were blocked with 1 % bovine serum albumin and 10 % donkey serum at room temperature for 1 h and then incubated at 4 °C overnight with primary antibodies for neuronal nuclear protein (NeuN) (1:500, Abcam, ab104224), ionized calcium-binding adapter molecule 1 (IBA-1) (1:500, Abcam, ab5076), glial fibrillary acidic protein (GFAP) (1:500, Millipore, MAB360), FXR (1:200, Proteintech, 25055–1-AP), MMP-2 (1:200, Proteintech, 10373-2-AP), MMP-9 (1:200, Proteintech, 10375-2-AP), PPAR-γ (1:200, Proteintech, 16643–1-AP), NLRP3 (1:200, Immunoway, YT5382), and cluster of differentiation 86 (CD86) (1:200, CST, #91882). ..

    Multiple Displacement Amplification:

    Article Title: A stage specific NETs-related signature in alcoholic steatohepatitis: from molecular subtyping to therapeutic vulnerabilities
    Article Snippet: .. Membranes were blocked and incubated overnight at 4 °C with primary antibodies against FOS (1:2000, Abclonal, A24620), MMP7 (1:1000, Proteintech, 10374-2-AP), CXCL6 (1:1000, Abclonal, TD13470), PPAR-α (1:500, Bioss, bs-23398R), PPAR-γ (1:1000, Proteintech, 16643-1-AP), HO-1 (1:1000, Abmart, PY5393S), MDA (1:1000, abcam, ab27642), Arg1 (1:5000, Proteintech, 16001-1-AP), MCP-1 (1:500, CST, 2029S), IL-10 (1:1000, Proteintech, 82191-3-RR) and β-actin (1:10,000, Proteintech, 20536-1-AP). .. Following incubation with HRP-conjugated secondary antibodies (1:8,000, Proteintech, SA00001-2), protein bands were visualized using a LI-COR imaging system.



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    Image Search Results


    Primers for qRT-PCR analyses.

    Journal: Frontiers in Genetics

    Article Title: HOTAIR regulates osteogenic–adipogenic differentiation imbalance through the miR-217/DKK1-mediated Wnt/β-catenin pathway in SONFH-BMSCs

    doi: 10.3389/fgene.2026.1917162

    Figure Lengend Snippet: Primers for qRT-PCR analyses.

    Article Snippet: The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies targeting Osterix (Boster, Cat. No. M02077, 1:1,000), BMP2 (Boster, Cat. No. BM4960, 1:1,000), RUNX2 (Boster, Cat. No. BM4700, 1:1,000), PPARγ (Boster, Cat. No. M00449-2, 1:1,000), C/EBPα (Boster, Cat. No. A00386-1, 1:1,000), Adipsin (Boster, Cat. No. M01134, 1:1,000), DKK1 (Boster, Cat. No. A09360-1, 1:1,000), OCT4 (Abmart, Cat. No. T55781 , 1:10,000), β-catenin (Abmart, Cat. No. M24002 , 1:10,000), and β-actin (Boster, Cat. No. BM3873, 1:10,000).

    Techniques: Sequencing

    HOTAIR silencing inhibits adipogenic differentiation of SONFH-BMSCs in vitro . (A) Oil Red O staining of FNF-derived control BMSCs (NC), untreated SONFH-BMSCs, SONFH si-NC, and SONFH si-HOTAIR groups at days 7 and 14. (B) Quantitative analysis of Oil Red O staining at days 7 and 14. (C) qRT-PCR analysis of PPARγ, C/EBPα, and Adipsin mRNA levels. Expression was normalized to GAPDH and is shown compared with untreated SONFH-BMSCs and si-NC controls. (D) Western blot analysis of PPARγ, C/EBPα, and Adipsin. Data are presented as mean ± SD from three independent biological replicates using BMSCs from independent donors, with three technical replicates per experiment. (*P < 0.05; **P < 0.01; ***P < 0.001).

    Journal: Frontiers in Genetics

    Article Title: HOTAIR regulates osteogenic–adipogenic differentiation imbalance through the miR-217/DKK1-mediated Wnt/β-catenin pathway in SONFH-BMSCs

    doi: 10.3389/fgene.2026.1917162

    Figure Lengend Snippet: HOTAIR silencing inhibits adipogenic differentiation of SONFH-BMSCs in vitro . (A) Oil Red O staining of FNF-derived control BMSCs (NC), untreated SONFH-BMSCs, SONFH si-NC, and SONFH si-HOTAIR groups at days 7 and 14. (B) Quantitative analysis of Oil Red O staining at days 7 and 14. (C) qRT-PCR analysis of PPARγ, C/EBPα, and Adipsin mRNA levels. Expression was normalized to GAPDH and is shown compared with untreated SONFH-BMSCs and si-NC controls. (D) Western blot analysis of PPARγ, C/EBPα, and Adipsin. Data are presented as mean ± SD from three independent biological replicates using BMSCs from independent donors, with three technical replicates per experiment. (*P < 0.05; **P < 0.01; ***P < 0.001).

    Article Snippet: The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies targeting Osterix (Boster, Cat. No. M02077, 1:1,000), BMP2 (Boster, Cat. No. BM4960, 1:1,000), RUNX2 (Boster, Cat. No. BM4700, 1:1,000), PPARγ (Boster, Cat. No. M00449-2, 1:1,000), C/EBPα (Boster, Cat. No. A00386-1, 1:1,000), Adipsin (Boster, Cat. No. M01134, 1:1,000), DKK1 (Boster, Cat. No. A09360-1, 1:1,000), OCT4 (Abmart, Cat. No. T55781 , 1:10,000), β-catenin (Abmart, Cat. No. M24002 , 1:10,000), and β-actin (Boster, Cat. No. BM3873, 1:10,000).

    Techniques: In Vitro, Staining, Derivative Assay, Control, Analysis, Quantitative RT-PCR, Expressing, Western Blot