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gl7 positive populations  (Oxford Instruments)
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Oxford Instruments gl7 positive populations
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Gl7 Positive Populations, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive controls  (Zymo Research)
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Zymo Research positive controls
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Positive Controls, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive expiratory pressure (pep) device  (Respironics)
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Respironics positive expiratory pressure (pep) device
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Positive Expiratory Pressure (Pep) Device, supplied by Respironics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive expiratory pressure (pep) device - by Bioz Stars, 2026-05
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exosome positive markers  (Exosome Diagnostics)
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Exosome Diagnostics exosome positive markers
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Exosome Positive Markers, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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exosome positive markers - by Bioz Stars, 2026-05
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centro parietal late positive component  (Finnigan Corporation)
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Finnigan Corporation centro parietal late positive component
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Centro Parietal Late Positive Component, supplied by Finnigan Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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centro parietal late positive component - by Bioz Stars, 2026-05
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z nano positioning stage  (Physik Instrumente)
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Physik Instrumente z nano positioning stage
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Z Nano Positioning Stage, supplied by Physik Instrumente, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/z nano positioning stage/product/Physik Instrumente
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z nano positioning stage - by Bioz Stars, 2026-05
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high dynamics z nano positioning stage  (Physik Instrumente)
86
Physik Instrumente high dynamics z nano positioning stage
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
High Dynamics Z Nano Positioning Stage, supplied by Physik Instrumente, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high dynamics z nano positioning stage - by Bioz Stars, 2026-05
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multi position magnetic stirrer  (Scientifica)
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Scientifica multi position magnetic stirrer
TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Multi Position Magnetic Stirrer, supplied by Scientifica, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multi position magnetic stirrer/product/Scientifica
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multi position magnetic stirrer - by Bioz Stars, 2026-05
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cd3 microbead positive selection  (Miltenyi Biotec)
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Miltenyi Biotec cd3 microbead positive selection
(A) Co-culture strategy (created with Biorender.com ). Small intestine (SI) was cryopreserved, and stable organoid lines were generated. T cells were isolated from SI tissue (B-E, H-K) or cord blood (F-G) by positive selection. <t>CD3+</t> cells were co-cultured with established SI organoid lines generated from fetal (B-I) , neonatal (D-E) , or adult (I-K) donors. (B, D, F, H) Representative images from day 6 of co-culture. Scale bar is 530 µm. (C) Data were generated from one organoid and T cell donor per fetal age group and analyzed by one-way ANOVA with Tukey’s post-hoc test. Only significant comparisons are denoted. Data are shown as means ± SEM. (E, G, I) Data were generated from 2-4 organoid and T cell donors each. Data were analyzed via Mann-Whitney and are shown as medians ± IQR. (K) Data were generated from 3 organoid donors and 4 T cell donors and are normalized to the number of organoids generated in mock (no T cell) conditions, represented as fold change. Each dot represents the average of three technical replicate wells (C) or one well of organoids (E, G, I, K) . Data were analyzed via Welch’s t-test and are shown as means ± SEM. Each experiment was repeated 2-3 independent times. Each dot represents one well of organoids. Significance is indicated as follows: * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001; ns, not significant (p>0.05).
Cd3 Microbead Positive Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 microbead positive selection - by Bioz Stars, 2026-05
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d816 position  (Mastocytosis Society)
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Mastocytosis Society d816 position
(A) Co-culture strategy (created with Biorender.com ). Small intestine (SI) was cryopreserved, and stable organoid lines were generated. T cells were isolated from SI tissue (B-E, H-K) or cord blood (F-G) by positive selection. <t>CD3+</t> cells were co-cultured with established SI organoid lines generated from fetal (B-I) , neonatal (D-E) , or adult (I-K) donors. (B, D, F, H) Representative images from day 6 of co-culture. Scale bar is 530 µm. (C) Data were generated from one organoid and T cell donor per fetal age group and analyzed by one-way ANOVA with Tukey’s post-hoc test. Only significant comparisons are denoted. Data are shown as means ± SEM. (E, G, I) Data were generated from 2-4 organoid and T cell donors each. Data were analyzed via Mann-Whitney and are shown as medians ± IQR. (K) Data were generated from 3 organoid donors and 4 T cell donors and are normalized to the number of organoids generated in mock (no T cell) conditions, represented as fold change. Each dot represents the average of three technical replicate wells (C) or one well of organoids (E, G, I, K) . Data were analyzed via Welch’s t-test and are shown as means ± SEM. Each experiment was repeated 2-3 independent times. Each dot represents one well of organoids. Significance is indicated as follows: * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001; ns, not significant (p>0.05).
D816 Position, supplied by Mastocytosis Society, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
d816 position - by Bioz Stars, 2026-05
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Image Search Results


TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using GL7, follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.

Journal: STAR Protocols

Article Title: Protocol for visualization and quantitative analysis of murine lung immunological architecture using iBALT-UNMAP following bacterial challenge

doi: 10.1016/j.xpro.2026.104472

Figure Lengend Snippet: TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using GL7, follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.

Article Snippet: Immune “Surfaces” tables for B220, CD3, and GL7 with object IDs and centroid coordinates. c. A lineage or class label per nucleus if you already assigned B cell, T cell, and GL7 positive populations in Imaris.

Techniques: Multiplex Assay, Staining, Incubation, Stripping Membranes

(A) Co-culture strategy (created with Biorender.com ). Small intestine (SI) was cryopreserved, and stable organoid lines were generated. T cells were isolated from SI tissue (B-E, H-K) or cord blood (F-G) by positive selection. CD3+ cells were co-cultured with established SI organoid lines generated from fetal (B-I) , neonatal (D-E) , or adult (I-K) donors. (B, D, F, H) Representative images from day 6 of co-culture. Scale bar is 530 µm. (C) Data were generated from one organoid and T cell donor per fetal age group and analyzed by one-way ANOVA with Tukey’s post-hoc test. Only significant comparisons are denoted. Data are shown as means ± SEM. (E, G, I) Data were generated from 2-4 organoid and T cell donors each. Data were analyzed via Mann-Whitney and are shown as medians ± IQR. (K) Data were generated from 3 organoid donors and 4 T cell donors and are normalized to the number of organoids generated in mock (no T cell) conditions, represented as fold change. Each dot represents the average of three technical replicate wells (C) or one well of organoids (E, G, I, K) . Data were analyzed via Welch’s t-test and are shown as means ± SEM. Each experiment was repeated 2-3 independent times. Each dot represents one well of organoids. Significance is indicated as follows: * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001; ns, not significant (p>0.05).

Journal: bioRxiv

Article Title: Early-life mucosal T cells direct intestinal stem cell fate via a coordinated developmental program

doi: 10.64898/2026.05.08.723752

Figure Lengend Snippet: (A) Co-culture strategy (created with Biorender.com ). Small intestine (SI) was cryopreserved, and stable organoid lines were generated. T cells were isolated from SI tissue (B-E, H-K) or cord blood (F-G) by positive selection. CD3+ cells were co-cultured with established SI organoid lines generated from fetal (B-I) , neonatal (D-E) , or adult (I-K) donors. (B, D, F, H) Representative images from day 6 of co-culture. Scale bar is 530 µm. (C) Data were generated from one organoid and T cell donor per fetal age group and analyzed by one-way ANOVA with Tukey’s post-hoc test. Only significant comparisons are denoted. Data are shown as means ± SEM. (E, G, I) Data were generated from 2-4 organoid and T cell donors each. Data were analyzed via Mann-Whitney and are shown as medians ± IQR. (K) Data were generated from 3 organoid donors and 4 T cell donors and are normalized to the number of organoids generated in mock (no T cell) conditions, represented as fold change. Each dot represents the average of three technical replicate wells (C) or one well of organoids (E, G, I, K) . Data were analyzed via Welch’s t-test and are shown as means ± SEM. Each experiment was repeated 2-3 independent times. Each dot represents one well of organoids. Significance is indicated as follows: * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001; ns, not significant (p>0.05).

Article Snippet: T cells were isolated via CD3 microbead positive selection (Miltenyi, cat#130-050-101 or 130-097-043) via MS or LS columns (Miltenyi, cat#130-042-201 or 130-042-401) per manufacturer’s instructions (Miltenyi).

Techniques: Co-Culture Assay, Generated, Isolation, Selection, Cell Culture, MANN-WHITNEY