Journal: Research in Pharmaceutical Sciences

Article Title: Expression and purification of SARS-CoV-2 receptor binding domain in Escherichia coli for diagnostic and therapeutic purposes

doi: 10.4103/RPS.RPS_93_23

Figure Lengend Snippet: Characterization of expressed recombinant RBD by western blotting. (A) Western blot of RBD protein using anti-His-tag rabbit antibody; (B): detection of commercial human ACE2 by recombinant RBD. RBD, receptor binding domain.

Article Snippet: Human anti-RBD antibodies obtained from the serum of convalescent patients diagnosed as COVID-19 positive (gifted from the Department of Virology, Pasteur Institute of Iran), rabbit IgG anti-SARS-CoV-2 RBD, and RBD immunized camel sera (gifted from Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran) in serial dilution of (1/2, 1/4, 1/8, 1/16, and 1/32) were added to the wells and the plates were incubated for 1 h at room temperature followed by three washes with PBS.

Techniques: Recombinant, Western Blot, Binding Assay

Journal: Research in Pharmaceutical Sciences

Article Title: Expression and purification of SARS-CoV-2 receptor binding domain in Escherichia coli for diagnostic and therapeutic purposes

doi: 10.4103/RPS.RPS_93_23

Figure Lengend Snippet: The reaction between the recombinant RBD and rabbit IgG anti-SARS-CoV-2 RBD resulted in a significant positive response at 450 nm. An immune response to the RBD was detected in the sample of immunized camel serum. Antibodies from the serum of convalescent patients diagnosed as COVID-19 positive showed binding ability to recombinant RBD. The ** P ≤ 0.01 and *** P ≤ 0.001 indicate significant differences compared to the respective control group (uncoated wells). RBD, receptor binding domain.

Article Snippet: Human anti-RBD antibodies obtained from the serum of convalescent patients diagnosed as COVID-19 positive (gifted from the Department of Virology, Pasteur Institute of Iran), rabbit IgG anti-SARS-CoV-2 RBD, and RBD immunized camel sera (gifted from Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran) in serial dilution of (1/2, 1/4, 1/8, 1/16, and 1/32) were added to the wells and the plates were incubated for 1 h at room temperature followed by three washes with PBS.

Techniques: Recombinant, Binding Assay, Control

Journal: Research in Pharmaceutical Sciences

Article Title: Expression and purification of SARS-CoV-2 receptor binding domain in Escherichia coli for diagnostic and therapeutic purposes

doi: 10.4103/RPS.RPS_93_23

Figure Lengend Snippet: Detection of RBD binding activity on human ACE2 receptor expressed in HEK293A cells by flow cytometry. The green signal corresponds to cells expressing ACE2 interacting with RBD. The red signal corresponds to commercial anti-ACE2 interacting with the ACE2 receptor expressed in HEK293A and the cyan signal corresponds to unlabeled ACE2/HEK293A cells. RBD, receptor binding domain; ACE2, angiotensin-converting enzyme 2.

Article Snippet: Human anti-RBD antibodies obtained from the serum of convalescent patients diagnosed as COVID-19 positive (gifted from the Department of Virology, Pasteur Institute of Iran), rabbit IgG anti-SARS-CoV-2 RBD, and RBD immunized camel sera (gifted from Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran) in serial dilution of (1/2, 1/4, 1/8, 1/16, and 1/32) were added to the wells and the plates were incubated for 1 h at room temperature followed by three washes with PBS.

Techniques: Binding Assay, Activity Assay, Flow Cytometry, Expressing

Journal: Vaccines

Article Title: Solid Organ Rejection following SARS-CoV-2 Vaccination or COVID-19 Infection: A Systematic Review and Meta-Analysis

doi: 10.3390/vaccines10081289

Figure Lengend Snippet: Summary of the characteristics of the included studies with evidence on organ rejection post-COVID-19 infection ( n = 20 studies), 2020–2022.

Article Snippet: Mohamed et al. 2021 [ ], United States , Retrospective case report, single centre , 33 , 0 [0] , 1 White (Caucasian) , rt-PCR and IgG anti SARS-CoV-2 [ n = 1] , 1 Shortness of breath 1 Pulse-oximetry (SpO2) ranging from 55–78% 1 Hypoxia 1 Tachypnea 1 Labored breathing 1 2 plus pitting oedema , 5 , 1 Congenital single kidney 1 Minimal change disease 1 Non-ischemic cardiomyopathy 1 Mitral valve repair 1 Obstructive sleep apnea 1 Failed living-related kidney transplant 1 Ureteric stent , 1 Acute kidney injury 1 Isolated vasculitis , 1 High creatinine 1 High D-dimer 1 Hematuria 1 1 Isolation of E. Faecium (bacteriuria) , Histopathological features were consistent with ARCR [ n = 1] , New diffuse airspace opacities [ n = 1] Severe intimal arteritis and hyperplasia [ n = 1] , 1 Endotracheal intubation 1 Mechanical ventilation 1 Bilevel positive airway pressure 1 Convalescent plasma 1 Remdesivir 1 Antibiotics 1 Oxygen supplementation 1 Steroid , (NOS, 8) No [ n = 1] 1 survived.

Techniques: Infection, Imaging, Reverse Transcription Polymerase Chain Reaction, Sedimentation, Isolation, Injection