Journal: STAR Protocols

Article Title: Protocol for visualization and quantitative analysis of murine lung immunological architecture using iBALT-UNMAP following bacterial challenge

doi: 10.1016/j.xpro.2026.104472

Figure Lengend Snippet: TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using GL7, follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.

Article Snippet: Immune “Surfaces” tables for B220, CD3, and GL7 with object IDs and centroid coordinates. c. A lineage or class label per nucleus if you already assigned B cell, T cell, and GL7 positive populations in Imaris.

Techniques: Multiplex Assay, Staining, Incubation, Stripping Membranes

Journal: Molecular Therapy Oncology

Article Title: Stem cell-directed targeted chemotherapy primes drug-resistant metastatic ovarian tumors for elimination by natural killer cells

doi: 10.1016/j.omton.2026.201214

Figure Lengend Snippet: Evaluation of MICA/B expression on the surface of CSCs and its correlation with sensitivity to laNK92 cells (A–F) Flow cytometry analysis of OV-031-T, OV-172-M, and OV-054-R ovarian CSCs extracted from tumorspheres before (Ctrl) and after treatment with SN38 or 5-FU. n = 3 flasks, biological replicates. (G–I) Measurement of NK cell-mediated toxicity to PDCs using a cell viability assay. This figure shows that treatment of CSCs with SN38 or 5-FU upregulates expression of MICA/B on CSCs making them more vulnerable to NK cells. The data are presented as mean ± SD, n = 3 wells, biological replicates, ∗∗ p < 0.01.

Article Snippet: Three days post-transduction, the transduced OV-031-T, OV-172-M, or OV-054-R cell populations were treated with puromycin (InvivoGen, Cat# ant-pr-1) at final concentrations of 2, 0.5, and 1 μg/mL, respectively, for 1 week to select for fLuc-expressing cells.

Techniques: Expressing, Flow Cytometry, Viability Assay

Journal: Molecular Therapy Oncology

Article Title: Stem cell-directed targeted chemotherapy primes drug-resistant metastatic ovarian tumors for elimination by natural killer cells

doi: 10.1016/j.omton.2026.201214

Figure Lengend Snippet: Evaluation of the ability of laNK92 cells to eliminate SN38/5-FU-treated metastatic ovarian cancer cells in vivo (A, D, G) Bioluminescent imaging of CIEA NOG mice that were implanted with untreated and drug-treated OV-031-T n = 3, OV-172-M n = 4, and OV-054-R n = 4 cancer cells, followed by i.p. injection of laNK92 cells. (B, E, H) qBLI of the change in tumor burden (untreated and drug-treated) after injection of laNK92 cells over the 30-day period. (C, F, I) Statistical analysis of fold change in BLI between untreated and drug-treated cancer cells after NK therapy. This fold change in BLI corresponds to the change in tumor burden over the 30-day period. Data are presented as mean ± SD, t test, ∗ p < 0.05.

Article Snippet: Three days post-transduction, the transduced OV-031-T, OV-172-M, or OV-054-R cell populations were treated with puromycin (InvivoGen, Cat# ant-pr-1) at final concentrations of 2, 0.5, and 1 μg/mL, respectively, for 1 week to select for fLuc-expressing cells.

Techniques: In Vivo, Imaging, Injection

Journal: Molecular Therapy Oncology

Article Title: Stem cell-directed targeted chemotherapy primes drug-resistant metastatic ovarian tumors for elimination by natural killer cells

doi: 10.1016/j.omton.2026.201214

Figure Lengend Snippet: Bioluminescent imaging of OV-031-T, OV-172-M, and OV-054-R ovarian tumors, shown in green, and ASC-shCE2:yCD cells, shown in red, in mice over a three-day period Post euthanasia, OV-031-T (A), OV-172-M (B), and OV-054-R (C) tumors and organs were removed and imaged after addition of furimazine, which is a substrate for nLuc. This figure shows active localization of ASC-shCE2:yCD cells in ovarian tumors, validating their tumor tropic properties; n = 1 mouse per cell line.

Article Snippet: Three days post-transduction, the transduced OV-031-T, OV-172-M, or OV-054-R cell populations were treated with puromycin (InvivoGen, Cat# ant-pr-1) at final concentrations of 2, 0.5, and 1 μg/mL, respectively, for 1 week to select for fLuc-expressing cells.

Techniques: Imaging

Journal: Molecular Therapy Oncology

Article Title: Stem cell-directed targeted chemotherapy primes drug-resistant metastatic ovarian tumors for elimination by natural killer cells

doi: 10.1016/j.omton.2026.201214

Figure Lengend Snippet: Evaluation of therapy responses and cancer relapses by BLI in CIEA NOG mice bearing OV-054-R tumors (A) qBLI of mice in the untreated group, n = 5 mice. (B) qBLI of mice treated with irinotecan, 25 mg/kg, and 5-FC, 350 mg/kg, twice per week, n = 5 mice. (C) qBLI of mice treated with ASC-shCE2:yCD cells once per week plus irinotecan, 35 mg/kg, and 5-FC, 350 mg/kg, twice per week for 11 weeks, followed by 5 weeks of therapy with laNK92-nLuc cells, n = 4, 1 additional mouse for IHC. The blue arrows point to the days laNK92-nLuc cells were injected. The number of animals per group was estimated based on pilot studies, providing sufficient power to avoid type I and type II errors. The dashed line at 1 ×10 6 , radiance, shows the background bioluminescence of mice without any tumors. (D) IHC of excised OV-054-R tumor tissue from the ASC-treated group versus the untreated control group using an anti-GFP primary antibody. (E) BLI, nLuc imaging, of laNK92-nLuc cells, validating the viability of the NK cells during the immunotherapy phase. (F) Measurement of mouse body weight during the immunotherapy phase.

Article Snippet: Three days post-transduction, the transduced OV-031-T, OV-172-M, or OV-054-R cell populations were treated with puromycin (InvivoGen, Cat# ant-pr-1) at final concentrations of 2, 0.5, and 1 μg/mL, respectively, for 1 week to select for fLuc-expressing cells.

Techniques: Injection, Control, Imaging

Journal: PeerJ

Article Title: A specialist surrounded by suburbia: the ecology of a newly described Four-toed Salamander population in New Brunswick

doi: 10.7717/peerj.21370

Figure Lengend Snippet: (A) Dorsal view of a Four-toed Salamander captured during this study (picture taken by Joshua Christiansen) on the left that is paired with (on the right) a picture of the ventral side of a different salamander from the site annotated with reference points and ellipses from the pattern recognition software I 3 S Spot ( v. 4.0.2). (B) Overview of the study site surrounded by suburban development created in Google Earth ® (accessed 1 April 2025; the map includes data from Google ® and imagery from 22 September 2017). The arrow points towards north. (C and D) Examples of anthropogenic disturbance in the Riverview High School study site highlighting litter. (E) Mulch laid down within the site to create human walking paths and a frisbee golf course.

Article Snippet: While there are some examples of Four-toed Salamander populations near anthropogenic development ( e.g. , ; ) the Riverview population, surrounded by anthropogenic landscapes ( e.g. , roads, parking lots, and suburban spawl), would appear to be an extreme of the urbanised populations published to-date.

Techniques: Software

Journal: PeerJ

Article Title: A specialist surrounded by suburbia: the ecology of a newly described Four-toed Salamander population in New Brunswick

doi: 10.7717/peerj.21370

Figure Lengend Snippet: (A) Substrate temperature (°C) recorded at each location ( V = 45, p = 0.001; Wilcoxon signed rank test with a continuity correction). The square points are means, the error bars are 95% confidence intervals, the points on the left of the error bars are raw data, and half violin plots are on the right of the error bars. (B) Principal component analysis visualization. Principal component one (PC1) is plotted along the x -axis explaining 40.30% of the total variance. PC1 reflects variation in soil moisture, soil pH, and canopy cover with loadings of −0.626, 0.567, 0.535, respectively. Principal component two (PC2) is plotted along the y -axis explaining 28.23% of the variance. PC2 reflects variation in substrate temperature with a loading of 0.892. The 95% confidence ellipses encapsulate data groupings for locations with salamanders vs. random locations.

Article Snippet: While there are some examples of Four-toed Salamander populations near anthropogenic development ( e.g. , ; ) the Riverview population, surrounded by anthropogenic landscapes ( e.g. , roads, parking lots, and suburban spawl), would appear to be an extreme of the urbanised populations published to-date.

Techniques:

Journal: PeerJ

Article Title: A specialist surrounded by suburbia: the ecology of a newly described Four-toed Salamander population in New Brunswick

doi: 10.7717/peerj.21370

Figure Lengend Snippet: Principal component one (PC1) is plotted along the x -axis with its corresponding PC scores, explaining 30.20% of the total variance. PC1 is dictated primarily by wooden debris and leaf/needle litter with loadings of −0.708 and 0.635, respectively. Principal component two (PC2) is plotted along the y -axis with its corresponding PC scores, explaining 25.73% of the variance. PC2 is dictated primarily by woody plants, herbaceous plants (herbaceous), and trees with loadings of 0.668, −0.316, −0.616, respectively. The 95% confidence ellipses encapsulate data groupings by type (salamander and random locations).

Article Snippet: While there are some examples of Four-toed Salamander populations near anthropogenic development ( e.g. , ; ) the Riverview population, surrounded by anthropogenic landscapes ( e.g. , roads, parking lots, and suburban spawl), would appear to be an extreme of the urbanised populations published to-date.

Techniques: