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A-C: Effect of NSAID on leukocyte migration: changes of migratory activity of isolated human <t>lymphocytes</t> and granulocytes were measured using 96-wells calcein AM assay . Ketoprofen (A), diclofenac (B) or SC-560 (C) showed a dose-dependent inhibition of PMNs and lymphocyte migration. D-F: Toxic effect of NSAID on PMNs: Leukocytes were incubated for 60 min with different concentrations of ketoprofen (D), diclofenac (E) and SC-560 (F). Cell number was measured using Calcein AM uptake. Only highest concentrations of ketoprofen (4 mM) and diclofenac (2.5-5 mM) induced significant reduction of the leukocyte number.
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A-C: Effect of NSAID on leukocyte migration: changes of migratory activity of isolated human lymphocytes and granulocytes were measured using 96-wells calcein AM assay . Ketoprofen (A), diclofenac (B) or SC-560 (C) showed a dose-dependent inhibition of PMNs and lymphocyte migration. D-F: Toxic effect of NSAID on PMNs: Leukocytes were incubated for 60 min with different concentrations of ketoprofen (D), diclofenac (E) and SC-560 (F). Cell number was measured using Calcein AM uptake. Only highest concentrations of ketoprofen (4 mM) and diclofenac (2.5-5 mM) induced significant reduction of the leukocyte number.

Journal: BMC Immunology

Article Title: Blockade of leukocyte haptokinesis and haptotaxis by ketoprofen, diclofenac and SC-560

doi: 10.1186/1471-2172-12-64

Figure Lengend Snippet: A-C: Effect of NSAID on leukocyte migration: changes of migratory activity of isolated human lymphocytes and granulocytes were measured using 96-wells calcein AM assay . Ketoprofen (A), diclofenac (B) or SC-560 (C) showed a dose-dependent inhibition of PMNs and lymphocyte migration. D-F: Toxic effect of NSAID on PMNs: Leukocytes were incubated for 60 min with different concentrations of ketoprofen (D), diclofenac (E) and SC-560 (F). Cell number was measured using Calcein AM uptake. Only highest concentrations of ketoprofen (4 mM) and diclofenac (2.5-5 mM) induced significant reduction of the leukocyte number.

Article Snippet: Polymorphonuclear leukocytes (PMNs) or lymphocytes were isolated from human venous peripheral blood using density gradient centrifugation (Biocoll, Biochrom AG, Berlin, Germany).

Techniques: Migration, Activity Assay, Isolation, Calcein AM Assay, Inhibition, Incubation

Mean IC50 (μM) for inhibition of leukocyte migration by NSAID.

Journal: BMC Immunology

Article Title: Blockade of leukocyte haptokinesis and haptotaxis by ketoprofen, diclofenac and SC-560

doi: 10.1186/1471-2172-12-64

Figure Lengend Snippet: Mean IC50 (μM) for inhibition of leukocyte migration by NSAID.

Article Snippet: Polymorphonuclear leukocytes (PMNs) or lymphocytes were isolated from human venous peripheral blood using density gradient centrifugation (Biocoll, Biochrom AG, Berlin, Germany).

Techniques: Inhibition, Migration