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Nacalai polybrene
Polybrene, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polybrene/polybrene/pmc13264021-415-14-15
Average 86 stars, based on 1 article reviews
polybrene - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Transduction:

Article Title: DHODH regulates trophoblast fusion via IFITM-reduced plasma membrane fluidity: Implications for hypertensive disorders of pregnancy
Article Snippet: .. BeWo cells were transduced with the viral particles in the presence of 10 μg/mL polybrene (Nacalai Tesque) and selected with 3 μg/mL puromycin (Nacalai Tesque) for 2 days. ..

Article Title: Targeting the CHD3 chromatin remodeler exploits a synthetic lethal vulnerability in dual SMARCA4/SMARCA2-deficient cancers via derepression of PARD3B.
Article Snippet: Lentivirus-containing supernatants were harvested and concentrated by centrifugation using Lenti-X Concentrator (Takara; 631232). .. To establish the H23 isogenic model, SMARCA4-deficient H23 cells were transduced with lentiviral particles carrying human SMARCA4 (pLV[Exp]-PuroCMV>hSMARCA4[NM_001128849.1]; Vector Builder, VB181029-1161teb) in the presence of 8 μg/mL polybrene (Nacalai Tesque; 12996-81). ..

Article Title: DHODH regulates trophoblast fusion via IFITM-reduced plasma membrane fluidity: Implications for hypertensive disorders of pregnancy
Article Snippet: These vectors were packaged in Lenti-X 293T cells using the Lentiviral High Titer Packaging Mix (Takara Bio) and TransIT-293 Transfection Reagent (Takara Bio) following the manufacturer’s instructions. .. The resulting lentiviral supernatants were filtered and used to transduce BeWo cells in the presence of 10 μg/mL polybrene (Nacalai Tesque). ..

other:

Article Title: Sarcopenia promotes tumorigenesis by disrupting NOTCH-SDC2-regulated biogenesis of muscle-derived extracellular vesicles.
Article Snippet: Three days after transfection, the lentivirus-containing supernatant was collected, centrifuged to remove cell debris, and filtered through a 0.45 μm filter (Sartorius, S6555).

Article Title: Characterization of novel macrophage cell lines derived from goat and sheep blood and their susceptibility to ruminant virus infections.
Article Snippet: GBMs or OBMs seeded on 60-mm NTC-dishes were exposed to these lentiviral particles in the presence of 6 μg/mL of Polybrene (Nacalai Tesque), and iGBM or iOBM was subsequently established after 1–2 months of culture.

Cell Culture:

Article Title: ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.
Article Snippet: To prepare the lentivirus, the pCSII-based lentiviral vector63, psPAX2 (Addgene plasmid #12260), and pCMVVSV-G-RSV-Rev were cotransfected into Lenti-X 293T cells. .. Host cells were cultured in the culture medium containing the lentivirus and 10 μg/mL polybrene (Nacalai Tesque) for 3–6 h. Instead of lentiviral transfer, a PiggyBac or Tol2 transposon system was used to establish stable cell lines expressing EKAREV-NLS, mScarlet-I-ZO-1, mScarlet-I-ZO-1ΔABR, mScarlet-I-ZO1Δphos or Tks5-mNeonGreen. .. The pPBbsr2- and pT2A-based vectors were cotransfected with pCAGGS-hyPBase and pCAGGS-T2TP encoding the Piggybac and Tol2 transposase, respectively, by using an Amaxa nucleofector system (Lonza) at a 4:1 ratio.

Stable Transfection:

Article Title: ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.
Article Snippet: To prepare the lentivirus, the pCSII-based lentiviral vector63, psPAX2 (Addgene plasmid #12260), and pCMVVSV-G-RSV-Rev were cotransfected into Lenti-X 293T cells. .. Host cells were cultured in the culture medium containing the lentivirus and 10 μg/mL polybrene (Nacalai Tesque) for 3–6 h. Instead of lentiviral transfer, a PiggyBac or Tol2 transposon system was used to establish stable cell lines expressing EKAREV-NLS, mScarlet-I-ZO-1, mScarlet-I-ZO-1ΔABR, mScarlet-I-ZO1Δphos or Tks5-mNeonGreen. .. The pPBbsr2- and pT2A-based vectors were cotransfected with pCAGGS-hyPBase and pCAGGS-T2TP encoding the Piggybac and Tol2 transposase, respectively, by using an Amaxa nucleofector system (Lonza) at a 4:1 ratio.

Expressing:

Article Title: ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.
Article Snippet: To prepare the lentivirus, the pCSII-based lentiviral vector63, psPAX2 (Addgene plasmid #12260), and pCMVVSV-G-RSV-Rev were cotransfected into Lenti-X 293T cells. .. Host cells were cultured in the culture medium containing the lentivirus and 10 μg/mL polybrene (Nacalai Tesque) for 3–6 h. Instead of lentiviral transfer, a PiggyBac or Tol2 transposon system was used to establish stable cell lines expressing EKAREV-NLS, mScarlet-I-ZO-1, mScarlet-I-ZO-1ΔABR, mScarlet-I-ZO1Δphos or Tks5-mNeonGreen. .. The pPBbsr2- and pT2A-based vectors were cotransfected with pCAGGS-hyPBase and pCAGGS-T2TP encoding the Piggybac and Tol2 transposase, respectively, by using an Amaxa nucleofector system (Lonza) at a 4:1 ratio.

Infection:

Article Title: An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation.
Article Snippet: .. Next, HEK293T cells in 6-well plates were infected with 10 μL of lentivirus in 10 μg/mL polybrene (#12996-81, Nacalai Tesque). ..

Virus:

Article Title: FXR maintains primordial follicle dormancy via regulating FoxO3 expression.
Article Snippet: 1 Reproduction 2 3 FXR maintains primordial follicle dormancy via regulating FoxO3 expression.. 4 5 Ikuo Tomioka*, Asuka Komoshita, Koichi Watanabe, Ayaka Ishihara, Hiromi Toda, Mitsuo 6 Ota, Yoshiaki Otani, Saki Ota, Siqin Gaowa, Hikaru Kousaku, Hiroshi Fujii, Yuji Takagi, 7 Takakazu Mitani, Kanako Morohaku.. 8 9 Faculty of Agriculture, Shinshu University.

Incubation:

Article Title: FXR maintains primordial follicle dormancy via regulating FoxO3 expression.
Article Snippet: 1 Reproduction 2 3 FXR maintains primordial follicle dormancy via regulating FoxO3 expression.. 4 5 Ikuo Tomioka*, Asuka Komoshita, Koichi Watanabe, Ayaka Ishihara, Hiromi Toda, Mitsuo 6 Ota, Yoshiaki Otani, Saki Ota, Siqin Gaowa, Hikaru Kousaku, Hiroshi Fujii, Yuji Takagi, 7 Takakazu Mitani, Kanako Morohaku.. 8 9 Faculty of Agriculture, Shinshu University.



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