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Schematic of the integrated optical system combining dual-wavelength confocal fluorescence excitation with spectral-domain OCT. CCD, charge-coupled device camera; CL, collimating lens; DM, dichroic mirror; FC, fiber coupler; F, filter; L, relay or imaging lens; LD, laser diode; PC, polarization controller; PMT, photomultiplier tube; SLD, superluminescent diode; SM, scanning mirrors. Confocal excitation at 445 nm and 561 nm is co-axially aligned with the OCT sample arm using shared galvanometric scanners, enabling intrinsically co-registered en face fluorescence and OCT acquisition. Retinal vascular contrast is derived from repeated OCT B-scans acquired at identical transverse locations.

Journal: Biomedical Optics Express

Article Title: Spatially resolved in vivo ratiometric imaging of mitochondrial quality control using co-registered confocal-OCT

doi: 10.1364/BOE.597380

Figure Lengend Snippet: Schematic of the integrated optical system combining dual-wavelength confocal fluorescence excitation with spectral-domain OCT. CCD, charge-coupled device camera; CL, collimating lens; DM, dichroic mirror; FC, fiber coupler; F, filter; L, relay or imaging lens; LD, laser diode; PC, polarization controller; PMT, photomultiplier tube; SLD, superluminescent diode; SM, scanning mirrors. Confocal excitation at 445 nm and 561 nm is co-axially aligned with the OCT sample arm using shared galvanometric scanners, enabling intrinsically co-registered en face fluorescence and OCT acquisition. Retinal vascular contrast is derived from repeated OCT B-scans acquired at identical transverse locations.

Article Snippet: The signal was subsequently collected by lenses (CL5 and CL6), spectrally filtered using an emission filter (F), and detected by a photomultiplier tube (PMT) (PMM01-1; Thorlabs, Inc., Newton, NJ, USA), as illustrated in .

Techniques: Fluorescence, Imaging, Derivative Assay