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plasmid encoding vsvg  (Addgene inc)
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Addgene inc plasmid encoding vsvg
<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Plasmid Encoding Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+vsvg/pmc12914541-221-24-27?v=Addgene+inc
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plasmid encoding vsvg - by Bioz Stars, 2026-07
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plasmid encoding the envelope of vesicular stomatitis virus (vsvg) (pmd.2g)  (Addgene inc)
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Addgene inc plasmid encoding the envelope of vesicular stomatitis virus (vsvg) (pmd.2g)
<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Plasmid Encoding The Envelope Of Vesicular Stomatitis Virus (Vsvg) (Pmd.2g), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid encoding the envelope of vesicular stomatitis virus (vsvg) (pmd.2g) - by Bioz Stars, 2026-07
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pmd2.g, vsvg encoding plasmid  (Addgene inc)
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Addgene inc pmd2.g, vsvg encoding plasmid
<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Pmd2.G, Vsvg Encoding Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+vsvg/pm38309274-98-214-225?v=Addgene+inc
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pmd2.g, vsvg encoding plasmid - by Bioz Stars, 2026-07
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vsvg encoding plasmid didier trono unpublished addgene plasmid  (Addgene inc)
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Addgene inc vsvg encoding plasmid didier trono unpublished addgene plasmid
<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Vsvg Encoding Plasmid Didier Trono Unpublished Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vsvg encoding plasmid didier trono unpublished addgene plasmid - by Bioz Stars, 2026-07
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pmd2 g envelope plasmids encoded vsvg glycoproteins  (Addgene inc)
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Addgene inc pmd2 g envelope plasmids encoded vsvg glycoproteins
<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Pmd2 G Envelope Plasmids Encoded Vsvg Glycoproteins, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmd2 g envelope plasmids encoded vsvg glycoproteins - by Bioz Stars, 2026-07
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plasmid encoding the envelope of vesicular stomatitis virus (vsvg; pmd.2g)  (Addgene inc)
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Addgene inc plasmid encoding the envelope of vesicular stomatitis virus (vsvg; pmd.2g)
<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Plasmid Encoding The Envelope Of Vesicular Stomatitis Virus (Vsvg; Pmd.2g), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid encoding the envelope of vesicular stomatitis virus (vsvg; pmd.2g) - by Bioz Stars, 2026-07
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expression plasmids encoding mutant cdk4, cyclin d1, csii-cmv-htert, pcmv-vsvg-rsv, pcag-hivgp, and csii-cmv-egfp  (BioResource International Inc)
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BioResource International Inc expression plasmids encoding mutant cdk4, cyclin d1, csii-cmv-htert, pcmv-vsvg-rsv, pcag-hivgp, and csii-cmv-egfp
<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Expression Plasmids Encoding Mutant Cdk4, Cyclin D1, Csii Cmv Htert, Pcmv Vsvg Rsv, Pcag Hivgp, And Csii Cmv Egfp, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expression plasmids encoding mutant cdk4, cyclin d1, csii-cmv-htert, pcmv-vsvg-rsv, pcag-hivgp, and csii-cmv-egfp - by Bioz Stars, 2026-07
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plp1, plp2, plp/vsvg plasmids encoding viral proteins gag-pol, rev, vsv-g  (Thermo Fisher)
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<t>HIV</t> infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus <t>(VSVG-pseudotyped</t> HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].
Plp1, Plp2, Plp/Vsvg Plasmids Encoding Viral Proteins Gag Pol, Rev, Vsv G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plp1, plp2, plp/vsvg plasmids encoding viral proteins gag-pol, rev, vsv-g - by Bioz Stars, 2026-07
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HIV infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].

Journal: Molecular Therapy. Nucleic Acids

Article Title: microRNA-422a promotes HIV replication and innate immune evasion by targeting MECP2

doi: 10.1016/j.omtn.2026.102844

Figure Lengend Snippet: HIV infection induces miR-422a in CD4+ T cells via expression of Nef (A) Primary CD4+ T cells were stimulated with anti-CD3/CD28 beads for 3 days, then infected with HIV-1 NL4-3 . The spreading infection was measured by evaluating supernatant p24 concentrations by ELISA at each time point. (B and C) The primary (B) and mature miR-422a expression levels (C) were measured by RT-qPCR after infection with HIV-1 in primary CD4+ T cells, as described in (A). (D) Stimulated primary CD4+ T cells were infected with an HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv EGFP reporter virus) for 3 days. Cells were then sorted based on EGFP expression using the Sony MA900 multi-application cell sorter and harvested for RNA isolation. miR-422a expression was measured by RT-qPCR. (E) Stimulated primary CD4+ T cells were infected with HIV-1 single-round reporter virus (VSVG-pseudotyped HIV NL4-3 ΔEnv ΔNef GFP reporter virus) for 3 days. Cells were then sorted based on GFP expression for RNA isolation. miR-422a expression was measured by RT-qPCR. (F) Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then collected to evaluate miR-422a expression by RT-qPCR. G. Nef was nucleofected into stimulated primary CD4+ T cells for 48 h. Cells were then stained with anti-CD25 and anti-69 antibodies to detect CD25 and CD69 expression by flow cytometry. Each dot represents data from one donor. Data are representative of the results of three independent experiments ( n = 3 biologically independent samples, mean ± SEM). Statistical significance was analyzed by unpaired or paired student t tests. p ≤ 0.05 [∗], p ≤ 0.01 [∗∗], p ≤ 0.001 [∗∗∗], p ≤ 0.0001 [∗∗∗∗].

Article Snippet: Specifically, VSVG-pseudotyped HIV NL4-3 ΔEnv EGFP reporter virus stocks were produced by co-transfecting the plasmid encoding HIV NL4-3 ΔEnv EGFP (BEI, #ARP-11100) and the plasmid encoding VSVG (Addgene, #8454) at a ratio of 3:1 into HEK-293T cells at 50%–60% confluency in culture flasks.

Techniques: Infection, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Virus, Isolation, Staining, Flow Cytometry