Journal: Plant Diversity

Article Title: The mitochondrial DnaJA protein LMA1 regulates reactive oxygen species homeostasis and anthocyanin biosynthesis in Medicago truncatula

doi: 10.1016/j.pld.2025.10.004

Figure Lengend Snippet: LMA1 is a mitochondria -localized DnaJA protein. (A) Phylogenetic analysis of the DnaJA protein from Medicago truncatula and other 183 representative species. Three main subtypes were named from DnaJA I to DnaJA III according to the well-supported branches in the phylogenetic tree and the variations of their J-domains (B). (B) Seqlogo plot of the J-domain from the three subtypes of DnaJA. Black arrowheads mark the visible variations of amino acids between these subtypes; red stars point the HPD motif; black rectangles indicate the absence of amino acids compared with the DnaJA I subtype. (C) Intergenomic gene collinearity (micro-synteny) analysis of LMA1 with ortholog in Amborella trichopoda (basalmost angiosperm), Aristolochia fimbriata (magnoliid), Vitis vinifera , and the Cercis chinensis (a species of the earliest-diverging subfamily of Fabaceae). (D) Subcellular localization of LMA1-GFP in protoplasts of Nicotiana benthamiana . Mitochondria were labeled with MitoTracker Red and showed in red fluorescence. Scale bar, 10 μm. (E) Computational prediction for the subcellular localization of three subtypes of DnaJA using nine high-accuracy methods (row name). Heatmap scale reflects the percent of DnaJA proteins which localizes in mitochondrion (mito), plastid/chloroplast (plastid), or other cellular compartments in each subtype. (F) Expression level of LMA1 in different tissues. Root, hypocotyl, stem, leaf, petiole, rachis, and shoot (vegetative stage) were collected from four-week-old plants, while flower and pod (five days post pollination) were sampled from eight-week-old plants. MtACTIN was used as internal control. Data are mean ± SD ( n = 3), one-way analysis of variance (ANOVA) with LSD post hoc test was adopted to compare independent samples, the different lowercase letters indicate significant differences ( P < 0.05).

Article Snippet: Here, we identified a novel player in the ROS-anthocyanin crosstalk signal in the model plant Medicago truncatula .

Techniques: Labeling, Fluorescence, Expressing, Control

Journal: Plant Diversity

Article Title: The mitochondrial DnaJA protein LMA1 regulates reactive oxygen species homeostasis and anthocyanin biosynthesis in Medicago truncatula

doi: 10.1016/j.pld.2025.10.004

Figure Lengend Snippet: Proposed model for LMA1-mediated ROS homeostasis and anthocyanin biosynthesis in Medicago truncatula . The mitochondrial-localized LMA1 protein maintains the cristae integrity and respiratory function for regulating ROS homeostasis and suppressing anthocyanin biosynthesis. Mutation of LMA1 leads to cristae disorganization, impairs mitochondrial complex I activity, and subsequent ROS overaccumulation, which activates anthocyanin biosynthetic pathway and pigment overaccumulation.

Article Snippet: Here, we identified a novel player in the ROS-anthocyanin crosstalk signal in the model plant Medicago truncatula .

Techniques: Mutagenesis, Activity Assay

Journal: Stress Biology

Article Title: Advances in CrRLK1L function in plant cell wall signaling during interaction with the environment and development

doi: 10.1007/s44154-025-00231-y

Figure Lengend Snippet: CrRLK1L members function in cell wall signaling and cell growth. A FER-mediated root cells burst in Arabidopsis (CWI context) (Feng et al. ). B FER-mediated reorganization of cortical microtubule assembly in Arabidopsis (CWI context)(Liu et al. ). C FER signaling in rhizoid growth in the liverwort (CWI context) (Westermann et al. ). D FER regulation of root hair development in Arabidopsis (CWI context) (Duan et al. ; Marzol et al. ; Schoenaers et al. ; Zhu et al. ). E FER regulation of fruit ripening in tomato (CWI context) (Jia et al. , ; Yin et al. ). F RALF1-FER regulation of flowering time in Arabidopsis (growth context) (Wang et al. ). G Mutation of FER involvement in regulating trichome development in Arabidopsis (growth context) (Duan et al. ). H FLR regulation of grain size in rice (growth context) (Wang et al. ). I The LRX3/4/5-RALF22/23-FER module regulates hormonal homeostasis and ROS accumulation in Arabidopsis (CWI context) (Zhao et al. ). J FER/THE1/HERK1 regulation of cell elongation in Arabidopsis (CWI context) (Guo et al. ). K FER, ANX1, and ANX2, BUPS1/2, ERU, CVY1 and ANJ regulation of pollen tube development in Arabidopsis (CWI context) (Boisson-Dernier et al. ; Escobar-Restrepo et al. ; Feng et al. ; Galindo‐Trigo et al . , ; Lan et al. ; Lassig et al. ). L FER-mediated lateral root development in Arabidopsis (growth context) (Dong et al. )

Article Snippet: The accumulated number of studies on CrRLK1L members involvement in cell wall signaling during plant development and response to the environment have significantly focused on genes involved in cell wall metabolism, especially in the model plant Arabidopsis (Boisson-Dernier et al. ; Gao and Li ; Nissen et al. ).

Techniques: Mutagenesis

Journal: Stress Biology

Article Title: Advances in CrRLK1L function in plant cell wall signaling during interaction with the environment and development

doi: 10.1007/s44154-025-00231-y

Figure Lengend Snippet: CrRLK1L members function in cell wall signaling and cell growth. A FER-mediated root cells burst in Arabidopsis (CWI context) (Feng et al. ). B FER-mediated reorganization of cortical microtubule assembly in Arabidopsis (CWI context)(Liu et al. ). C FER signaling in rhizoid growth in the liverwort (CWI context) (Westermann et al. ). D FER regulation of root hair development in Arabidopsis (CWI context) (Duan et al. ; Marzol et al. ; Schoenaers et al. ; Zhu et al. ). E FER regulation of fruit ripening in tomato (CWI context) (Jia et al. , ; Yin et al. ). F RALF1-FER regulation of flowering time in Arabidopsis (growth context) (Wang et al. ). G Mutation of FER involvement in regulating trichome development in Arabidopsis (growth context) (Duan et al. ). H FLR regulation of grain size in rice (growth context) (Wang et al. ). I The LRX3/4/5-RALF22/23-FER module regulates hormonal homeostasis and ROS accumulation in Arabidopsis (CWI context) (Zhao et al. ). J FER/THE1/HERK1 regulation of cell elongation in Arabidopsis (CWI context) (Guo et al. ). K FER, ANX1, and ANX2, BUPS1/2, ERU, CVY1 and ANJ regulation of pollen tube development in Arabidopsis (CWI context) (Boisson-Dernier et al. ; Escobar-Restrepo et al. ; Feng et al. ; Galindo‐Trigo et al . , ; Lan et al. ; Lassig et al. ). L FER-mediated lateral root development in Arabidopsis (growth context) (Dong et al. )

Article Snippet: The model plant Arabidopsis thaliana (Arabidopsis) has 17 CrRLKI1L subfamily members (Hématy and Höfte ), among which 8 have been well characterized to function in cell wall remodeling (Franck et al. ; Gandhi and Oelmüller ; Lindner et al. ; Nissen et al. ).

Techniques: Mutagenesis