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Ex vivo autoradiography and histochemical imaging of C6 glioma-bearing rat brains. After PET imaging using L- ( a ) or D- 18 F-FBPA ( b ), rats were sacrificed under anesthesia, the brain was excised and sliced at a thickness of 2 mm, and the slices were then exposed to a <t>phosphoimaging</t> plate in order to calculate the target tumor (glioma) to non-target (normal) ratios of L- and D- 18 F-FBPA ( c ). # P < 0.05 vs. L-isomer. The brain excised from another rat bearing C6 glioma was fixed in 4% neutral-buffered formalin; samples were embedded in paraffin, sliced at a thickness of 30 μm, and stained with HE ( d )
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Ex vivo autoradiography and histochemical imaging of C6 glioma-bearing rat brains. After PET imaging using L- ( a ) or D- 18 F-FBPA ( b ), rats were sacrificed under anesthesia, the brain was excised and sliced at a thickness of 2 mm, and the slices were then exposed to a phosphoimaging plate in order to calculate the target tumor (glioma) to non-target (normal) ratios of L- and D- 18 F-FBPA ( c ). # P < 0.05 vs. L-isomer. The brain excised from another rat bearing C6 glioma was fixed in 4% neutral-buffered formalin; samples were embedded in paraffin, sliced at a thickness of 30 μm, and stained with HE ( d )

Journal: EJNMMI Research

Article Title: Evaluation of D-isomers of 4-borono-2- 18 F-fluoro-phenylalanine and O - 11 C-methyl-tyrosine as brain tumor imaging agents: a comparative PET study with their L-isomers in rat brain glioma

doi: 10.1186/s13550-018-0404-6

Figure Lengend Snippet: Ex vivo autoradiography and histochemical imaging of C6 glioma-bearing rat brains. After PET imaging using L- ( a ) or D- 18 F-FBPA ( b ), rats were sacrificed under anesthesia, the brain was excised and sliced at a thickness of 2 mm, and the slices were then exposed to a phosphoimaging plate in order to calculate the target tumor (glioma) to non-target (normal) ratios of L- and D- 18 F-FBPA ( c ). # P < 0.05 vs. L-isomer. The brain excised from another rat bearing C6 glioma was fixed in 4% neutral-buffered formalin; samples were embedded in paraffin, sliced at a thickness of 30 μm, and stained with HE ( d )

Article Snippet: Slices were transferred to a phosphoimaging plate (BAS-IIIs, Fuji film) for 10 min of exposure with an authentic standard radioactivity source.

Techniques: Ex Vivo, Autoradiography, Imaging, Staining