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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Coreopsis tinctoria Nutt. attenuates ultraviolet A photodamage by suppressing endoplasmic reticulum stress-induced apoptosis via Nrf2 crosstalk
doi: 10.3389/fphar.2025.1686234
Figure Lengend Snippet: CT alleviates UVA-induced ER stress and apoptosis via Nrf2 in HaCaT cells. (A) HaCaT cells were subjected to UVA radiation and treated with 50 or 100 μg/mL CT and/or siNrf2. Western blot assays were performed to detect the levels of ATF6, phosphorylated elF2a (p-elF2a), CHOP, GADD34, Cleaved caspase9, Cleaved caspase3, JNK, phosphorylated JNK (p-JNK), Bax, BCL2, cytoplasmic and nuclear NRF2 and HO-1. (B) Flow cytometry analysis was performed to determine cell apoptosis. Quantification of the percentage of apoptotic cells in different treatments. (C) Heatmap generated the data from RNAseq. Genes are all related to NRF2 downstream anti-oxidant or detoxification target genes. The figure was generated by SRplot . N = 4. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
Article Snippet: The membranes were blocked with 5% non-fat milk and incubated with the following primary antibodies: IRE1 (phospho S724) (Abcam, ab124945); CHOP (Proteintech,15204-1-AP); NRF2 (Proteintech, 16396-1-AP); ATF6 (Proteintech, 24169-1-AP); PERK (phospho Thr982) (Proteintech, 82534-1-RR); eIF2α(phospho Ser51) (Proteintech, 68023-1-Ig); GADD34 (Proteintech, 10449-1-AP); Caspase9 (Proteintech, 10380-1-AP); Cleaved caspase3 (Proteintech, 25128-1-AP);
Techniques: Western Blot, Flow Cytometry, Generated