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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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p jnk fy er5754 118 elisa kit  (Wuhan Feiyue Biotechnology Co Ltd)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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jnk1 kinase assay kit  (Genmed Inc)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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jnk inhibitor sp600125  (MedChemExpress)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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jnk inhibitor viii  (MedChemExpress)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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Image Search Results


Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

Techniques: Gene Expression, Thermal Shift Assay, Expressing

The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

Techniques: Expressing