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Anti-spike IgG antibody levels in patient samples were measured using Abbott’s SARS-CoV-2 IgG II Quant <t>ARCHITECT</t> assay. Comparing these antibody concentrations, the one-dose J&J vaccine induces lower antibody levels than the two-dose Pfizer and Moderna vaccines. There is no significant difference in mean concentrations between individuals vaccinated with Pfizer vs Moderna (the Tukey HSD test p -value = 0.5108). However, there is a significant difference in antibody concentration levels across the three groups as determined by ANOVA (F-test p -value = 0.0001), and pairwise Tukey HSD (Pfizer vs J&J: p -value = 0.0019; Moderna vs J&J: p -value = 0.0000). The number of samples tested was 26, 19, and 29 for Pfizer, Johnson & Johnson, and Moderna, respectively.
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Anti-spike IgG antibody levels in patient samples were measured using Abbott’s SARS-CoV-2 IgG II Quant ARCHITECT assay. Comparing these antibody concentrations, the one-dose J&J vaccine induces lower antibody levels than the two-dose Pfizer and Moderna vaccines. There is no significant difference in mean concentrations between individuals vaccinated with Pfizer vs Moderna (the Tukey HSD test p -value = 0.5108). However, there is a significant difference in antibody concentration levels across the three groups as determined by ANOVA (F-test p -value = 0.0001), and pairwise Tukey HSD (Pfizer vs J&J: p -value = 0.0019; Moderna vs J&J: p -value = 0.0000). The number of samples tested was 26, 19, and 29 for Pfizer, Johnson & Johnson, and Moderna, respectively.

Journal: Communications Medicine

Article Title: Affinity of anti-spike antibodies to three major SARS-CoV-2 variants in recipients of three major vaccines

doi: 10.1038/s43856-022-00174-9

Figure Lengend Snippet: Anti-spike IgG antibody levels in patient samples were measured using Abbott’s SARS-CoV-2 IgG II Quant ARCHITECT assay. Comparing these antibody concentrations, the one-dose J&J vaccine induces lower antibody levels than the two-dose Pfizer and Moderna vaccines. There is no significant difference in mean concentrations between individuals vaccinated with Pfizer vs Moderna (the Tukey HSD test p -value = 0.5108). However, there is a significant difference in antibody concentration levels across the three groups as determined by ANOVA (F-test p -value = 0.0001), and pairwise Tukey HSD (Pfizer vs J&J: p -value = 0.0019; Moderna vs J&J: p -value = 0.0000). The number of samples tested was 26, 19, and 29 for Pfizer, Johnson & Johnson, and Moderna, respectively.

Article Snippet: ARCHITECT wash buffer (phosphate buffer containing detergent, Abbott Laboratories, Abbott Park, IL) served as the primary diluent for all experiments.

Techniques: Vaccines, Concentration Assay