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A, Western blot analysis of <t>PHB1</t> protein levels. B, Quantification analysis of the results shown in (A). C, RT‒qPCR was used to determine the mRNA level of PHB1. D, Western blot analysis of Flag protein levels. E, Western blot analysis of Bax and Bcl2 protein levels. F, Quantification analysis of the results shown in (D). G, Western blot analysis of C3 and Cc3 protein levels. H, Quantification analysis of the results shown in (F). I, TUNEL assay determined apoptosis of NMCMs in different groups. J, Quantification of the apoptosis rates shown in (H). K, CCK-8 assay was used to determine the viability of NMCMs in different groups. Data are the means ± SDs from 3 independent biological experiments. * P < 0.05, ** P < 0.01.
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A, Western blot analysis of <t>PHB1</t> protein levels. B, Quantification analysis of the results shown in (A). C, RT‒qPCR was used to determine the mRNA level of PHB1. D, Western blot analysis of Flag protein levels. E, Western blot analysis of Bax and Bcl2 protein levels. F, Quantification analysis of the results shown in (D). G, Western blot analysis of C3 and Cc3 protein levels. H, Quantification analysis of the results shown in (F). I, TUNEL assay determined apoptosis of NMCMs in different groups. J, Quantification of the apoptosis rates shown in (H). K, CCK-8 assay was used to determine the viability of NMCMs in different groups. Data are the means ± SDs from 3 independent biological experiments. * P < 0.05, ** P < 0.01.
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A, Western blot analysis of PHB1 protein levels. B, Quantification analysis of the results shown in (A). C, RT‒qPCR was used to determine the mRNA level of PHB1. D, Western blot analysis of Flag protein levels. E, Western blot analysis of Bax and Bcl2 protein levels. F, Quantification analysis of the results shown in (D). G, Western blot analysis of C3 and Cc3 protein levels. H, Quantification analysis of the results shown in (F). I, TUNEL assay determined apoptosis of NMCMs in different groups. J, Quantification of the apoptosis rates shown in (H). K, CCK-8 assay was used to determine the viability of NMCMs in different groups. Data are the means ± SDs from 3 independent biological experiments. * P < 0.05, ** P < 0.01.

Journal: Journal of Cardiovascular Pharmacology

Article Title: PHB1 Attenuates Triptolide-induced Cardiotoxicity by Regulating Mitochondrial Dynamics in Cultured Newborn Mice Cardiomyocytes

doi: 10.1097/FJC.0000000000001766

Figure Lengend Snippet: A, Western blot analysis of PHB1 protein levels. B, Quantification analysis of the results shown in (A). C, RT‒qPCR was used to determine the mRNA level of PHB1. D, Western blot analysis of Flag protein levels. E, Western blot analysis of Bax and Bcl2 protein levels. F, Quantification analysis of the results shown in (D). G, Western blot analysis of C3 and Cc3 protein levels. H, Quantification analysis of the results shown in (F). I, TUNEL assay determined apoptosis of NMCMs in different groups. J, Quantification of the apoptosis rates shown in (H). K, CCK-8 assay was used to determine the viability of NMCMs in different groups. Data are the means ± SDs from 3 independent biological experiments. * P < 0.05, ** P < 0.01.

Article Snippet: Antibodies against PHB1 (1:1000, 2426), Phospho-DRP1 (S616) (1:1000, 4494), DRP1 (1:1000, 8570), OPA1 (1:1000, 80471), Caspase-3 (1:1000, 9662), Cleaved caspase-3 (1:1000, 9661), Bax (1:1000, 2772), and Bcl2 (1:1000, 3498) were purchased from Cell Signaling Technology.

Techniques: Western Blot, TUNEL Assay, CCK-8 Assay