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GenScript corporation expression vector pgs21a
Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgs21a+expression+vector/pgs+21a/pmc03225368-39-6-9
Average 90 stars, based on 1 article reviews
expression vector pgs21a - by Bioz Stars, 2026-11
90/100 stars

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Sequencing:

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. Gene synthesis The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt P41243) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 50 NdeI and 30 XhoI sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript).

Expressing:

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. Gene synthesis The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt P41243) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 50 NdeI and 30 XhoI sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript).

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum .
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; Supplementary Figure S1). ..

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; ). ..

Synthesized:

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. Gene synthesis The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt P41243) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 50 NdeI and 30 XhoI sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript).

Plasmid Preparation:

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. Gene synthesis The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt P41243) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 50 NdeI and 30 XhoI sites and subcloned into the pGS21a expression vector (Genscript). ..

Article Title: Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase
Article Snippet: .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript). .. The coding sequence corresponding to residues 71–174 of Rattus norvegicus CHK (UniProt {"type":"entrez-protein","attrs":{"text":"P41243","term_id":"729890","term_text":"P41243"}} P41243 ) was optimized for Escherichia coli expression (OptimumGene algorithm, GenScript), synthesized with flanking 5′ Nde I and 3′ Xho I sites and subcloned into the pGS21a expression vector (Genscript).

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum .
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; Supplementary Figure S1). ..

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; ). ..

Produced:

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum .
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; Supplementary Figure S1). ..

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; ). ..

Clone Assay:

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum .
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; Supplementary Figure S1). ..

Article Title: Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum
Article Snippet: .. Briefly, a codon-optimized gene encoding Pf DXRt (UniProt Q8IKG4) was synthetically produced (GenScript Biotech B.V., Leiden, The Netherlands) and cloned into the pGS21a expression vector (GenScript Biotech B.V.; ). ..



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GenScript corporation expression vector pgs21a
Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgs21a+expression+vector/pgs+21a/pmc03225368-39-6-9
Average 90 stars, based on 1 article reviews
expression vector pgs21a - by Bioz Stars, 2026-11
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  Buy from Supplier

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GenScript corporation bacterial expression vector pgs21a
A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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GenScript corporation the bacterial expression vector pgs21a
A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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Average 90 stars, based on 1 article reviews
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90/100 stars
  Buy from Supplier

Image Search Results


A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, Recombinant, Plasmid Preparation

A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, Recombinant, Plasmid Preparation