pegfp n1 in pcdna3 0 (TaKaRa)

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TaKaRa pegfp n1 in pcdna3 0
Pegfp N1 In Pcdna3 0, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgfp+n1+vector/pGFP+Vector/pmc12141203-1-0-6
Average 94 stars, based on 386 article reviews

pegfp n1 in pcdna3 0 - by Bioz Stars, 2026-10

94/100 stars

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Clone Assay:

Article Title: Structural Basis of CD160:HVEM Recognition
Article Snippet: The gene encoding full length human CD160 was cloned into an engineered pmCherry-N1 vector (Clontech) to be expressed as a protein fused with the human PD-L1 transmembrane segment followed by the fluorophore mCherry at its C-terminus. .. The gene coding full length human HVEM was cloned into the pGFP-N1 vector (Clontech) for expression as a protein fused with fluorophore eGFP at the C-terminus. .. All CD160 and HVEM mutants were generated using QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies).

Article Title: Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming
Article Snippet: For the overexpression of FAM210B, recombinant lentivirus expressing myc-FAM210B and myc-control lentivirus were also from Shandong Vigene Biosciences of China .. Full-length FAM210b, N-terminal 47 aa and the 48–192 aa of FAM210b constructs were developed using human FAM210B complementary DNA clone (OriGene, Rockville, USA, SC124466) and PCR, and the PCR products were cloned into pGFP-N1 vector (Clontech, USA). .. Mitochondrial or endoplasmic reticulum morphology and fusion were observed using Mito-DsRed (Clontech) or ER-DsRed (Clontech).

Article Title: HVEM structures and mutants reveal distinct functions of binding to LIGHT and BTLA/CD160
Article Snippet: DNA fragment encoding the amino acid sequence “HHHHHHGG” fused to the N-terminus of the single chain homotrimeric mLIGHT extracellular domain (G73-V239) connecting by two (GGGGS)4 linkers was cloned into pcDNA 3.3 vector (Life technologies). .. A DNA fragment encoding residues of L39-V202 of hHVEM was cloned into an engineered pGFP-N1 vector (Clontech) for expression as a protein fused with a PD-L1 trans-membrane domain followed by the fluorophore eGFP at the C-terminus. .. The hHVEM mutant library was generated using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies).

Plasmid Preparation:

Article Title: Use of Bacterial Extracellular Vesicles for Gene Delivery to Host Cells
Article Snippet: .. The pGFP-N1 vector (Clontech, Mountain View, CA, USA) was used for EGFP plasmid DNA and in vitro EGFP transcription. .. The EGFP RNA was synthesized using the HeLaScribe Nuclear Extract in vitro Transcription System (Promega, Madison, WI, USA) with the plasmid gene (pGFP-N1) as a template, according to the manufacturer’s protocol.

Article Title: Use of Bacterial Extracellular Vesicles for Gene Delivery to Host Cells.
Article Snippet: .. The pGFP-N1 vector (Clontech, Mountain View, CA, USA) was used for EGFP plasmid DNA and in vitro EGFP transcription. .. The EGFP RNA was synthesized using the HeLaScribe Nuclear Extract in vitro Transcription System (Promega, Madison, WI, USA) with the plasmid gene (pGFP-N1) as a template, according to the manufacturer’s protocol.

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Transfection:

Article Title: High cofilin-1 levels correlate with cisplatin resistance in lung adenocarcinomas.
Article Snippet: High cofilin-1 levels have been shown to be an accurate prognostic biomarker in non-small cell lung cancer (NSCLC) and a predictive factor in drug resistance.. Herein we explore the role of cofilin-1 in cis diamminedichloroplatinum(II) (cisplatin) resistance.. We evaluated cofilin-1 levels in intrinsically cisplatinresistant A549 (ICR-A549) cells and determined the cisplatin toxicity in A549 cells transiently transfected and overexpressing CFL1 plasmid.

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Article Title: Structural Basis of CD160:HVEM Recognition
Article Snippet: Structural analysis benefited from the online server PDBePISA ( Krissinel and Henrick, 2007 ). . SPR to Test Binding Human HVEM protein derived from Drosophila S2 cells was immobilized on CM5 sensor chips (GE Life Sciences).

Review