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Oral infection with P. <t>gingivalis</t> induced periodontal tissue inflammation. (A) The representative blot showed the changes in iNOS expression within the periodontal tissues of mice in the control group and the P. gingivalis -infected group. (B) Bar graph with scatter points showed the quantification of iNOS in (A) . (C) Bar graph with scatter points showed the quantification of IL-1β mRNA in the periodontal tissues. (D) Bar graph with scatter points showed the quantification of IL-6 mRNA in the periodontal tissues. ** p < 0.01 and *** p < 0.001.
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Oral infection with P. <t>gingivalis</t> induced periodontal tissue inflammation. (A) The representative blot showed the changes in iNOS expression within the periodontal tissues of mice in the control group and the P. gingivalis -infected group. (B) Bar graph with scatter points showed the quantification of iNOS in (A) . (C) Bar graph with scatter points showed the quantification of IL-1β mRNA in the periodontal tissues. (D) Bar graph with scatter points showed the quantification of IL-6 mRNA in the periodontal tissues. ** p < 0.01 and *** p < 0.001.
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Oral infection with P. gingivalis induced periodontal tissue inflammation. (A) The representative blot showed the changes in iNOS expression within the periodontal tissues of mice in the control group and the P. gingivalis -infected group. (B) Bar graph with scatter points showed the quantification of iNOS in (A) . (C) Bar graph with scatter points showed the quantification of IL-1β mRNA in the periodontal tissues. (D) Bar graph with scatter points showed the quantification of IL-6 mRNA in the periodontal tissues. ** p < 0.01 and *** p < 0.001.

Journal: Frontiers in Neuroscience

Article Title: USP22 knockdown attenuates P. gingivalis -induced EndoMT and CNS inflammation: a link between periodontitis and neuroinflammation

doi: 10.3389/fnins.2026.1790970

Figure Lengend Snippet: Oral infection with P. gingivalis induced periodontal tissue inflammation. (A) The representative blot showed the changes in iNOS expression within the periodontal tissues of mice in the control group and the P. gingivalis -infected group. (B) Bar graph with scatter points showed the quantification of iNOS in (A) . (C) Bar graph with scatter points showed the quantification of IL-1β mRNA in the periodontal tissues. (D) Bar graph with scatter points showed the quantification of IL-6 mRNA in the periodontal tissues. ** p < 0.01 and *** p < 0.001.

Article Snippet: HBMECs were exposed to 100 ng/mL LPS from P. gingivalis (tlrl-pglps, InvivoGen) for 24 h.

Techniques: Infection, Expressing, Control

Oral infection with P. gingivalis induced EndoMT and inflammation in hippocampus. (A) The representative blots showed the changes in ZO-1, Claudin5, α -SMA, and iNOS expression within the hippocampus in the control group and the P. gingivalis -infected mice. (B) Bar graph with scatter points showed the quantification of ZO-1 in (A) . (C) Bar graph with scatter points showed the quantification of Claudin5 in (A) . (D) Bar graph with scatter points showed the quantification of α-SMA in (A) . (E) Bar graph with scatter points showed the quantification of iNOS in (A) . (F) Bar graph with scatter points showed the quantification of IL-1β mRNA in the hippocampus. (G) Bar graph with scatter points showed the quantification of IL-6 mRNA in the hippocampus. *** p < 0.001.

Journal: Frontiers in Neuroscience

Article Title: USP22 knockdown attenuates P. gingivalis -induced EndoMT and CNS inflammation: a link between periodontitis and neuroinflammation

doi: 10.3389/fnins.2026.1790970

Figure Lengend Snippet: Oral infection with P. gingivalis induced EndoMT and inflammation in hippocampus. (A) The representative blots showed the changes in ZO-1, Claudin5, α -SMA, and iNOS expression within the hippocampus in the control group and the P. gingivalis -infected mice. (B) Bar graph with scatter points showed the quantification of ZO-1 in (A) . (C) Bar graph with scatter points showed the quantification of Claudin5 in (A) . (D) Bar graph with scatter points showed the quantification of α-SMA in (A) . (E) Bar graph with scatter points showed the quantification of iNOS in (A) . (F) Bar graph with scatter points showed the quantification of IL-1β mRNA in the hippocampus. (G) Bar graph with scatter points showed the quantification of IL-6 mRNA in the hippocampus. *** p < 0.001.

Article Snippet: HBMECs were exposed to 100 ng/mL LPS from P. gingivalis (tlrl-pglps, InvivoGen) for 24 h.

Techniques: Infection, Expressing, Control

USP22 was upregulated in hippocampal tissues of P. gingivalis -infected mice and in LPS-exposed HBMECs. (A) The representative blot showed the changes in USP22 expression within the hippocampus in the control group and the P. gingivalis -infected mice. (B) Bar graph with scatter points showed the quantification of USP22 in (A) . (C) The representative blot showed the change in USP22 expression in the control and LPS-exposed HBMECs. (D) Bar graph with scatter points showed the quantification of USP22 in (C) . (E) The representative blot showed the change in USP22 expression in the control and USP22 HBMECs. (F) Bar graph with scatter points showed the quantification of USP22 in (E) . * p < 0.05, ** p < 0.01 and *** p < 0.001.

Journal: Frontiers in Neuroscience

Article Title: USP22 knockdown attenuates P. gingivalis -induced EndoMT and CNS inflammation: a link between periodontitis and neuroinflammation

doi: 10.3389/fnins.2026.1790970

Figure Lengend Snippet: USP22 was upregulated in hippocampal tissues of P. gingivalis -infected mice and in LPS-exposed HBMECs. (A) The representative blot showed the changes in USP22 expression within the hippocampus in the control group and the P. gingivalis -infected mice. (B) Bar graph with scatter points showed the quantification of USP22 in (A) . (C) The representative blot showed the change in USP22 expression in the control and LPS-exposed HBMECs. (D) Bar graph with scatter points showed the quantification of USP22 in (C) . (E) The representative blot showed the change in USP22 expression in the control and USP22 HBMECs. (F) Bar graph with scatter points showed the quantification of USP22 in (E) . * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: HBMECs were exposed to 100 ng/mL LPS from P. gingivalis (tlrl-pglps, InvivoGen) for 24 h.

Techniques: Infection, Expressing, Control

Endothelial-specific knockdown of USP22 inhibited EndoMT and hippocampal inflammation. (A) Representative images showed the colocalization of GFP signal (green) and CD31 immunofluorescence (red) in mouse hippocampus. Scale bar: 200 μm. (B) The representative blots showed the changes in ZO-1, Claudin5, α-SMA, and iNOS expression within the hippocampus in the control group and P. gingivalis -infected mice injected with USP22-shRNA AAV. (C) Bar graph with scatter points showed the quantification of USP22 in (A) . (D) Bar graph with scatter points showed the quantification of ZO-1 in (A) . (E) Bar graph with scatter points showed the quantification of Claudin5 in (A) . (F) Bar graph with scatter points showed the quantification of α-SMA in (A) . (G) Bar graph with scatter points showed the quantification of iNOS in (A) . (H,I) Bar graphs with scatter points showed the quantification of IL-1β mRNA (H) and IL-6 mRNA (I) in the control group and P. gingivalis -infected mice injected with USP22-shRNA AAV. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Neuroscience

Article Title: USP22 knockdown attenuates P. gingivalis -induced EndoMT and CNS inflammation: a link between periodontitis and neuroinflammation

doi: 10.3389/fnins.2026.1790970

Figure Lengend Snippet: Endothelial-specific knockdown of USP22 inhibited EndoMT and hippocampal inflammation. (A) Representative images showed the colocalization of GFP signal (green) and CD31 immunofluorescence (red) in mouse hippocampus. Scale bar: 200 μm. (B) The representative blots showed the changes in ZO-1, Claudin5, α-SMA, and iNOS expression within the hippocampus in the control group and P. gingivalis -infected mice injected with USP22-shRNA AAV. (C) Bar graph with scatter points showed the quantification of USP22 in (A) . (D) Bar graph with scatter points showed the quantification of ZO-1 in (A) . (E) Bar graph with scatter points showed the quantification of Claudin5 in (A) . (F) Bar graph with scatter points showed the quantification of α-SMA in (A) . (G) Bar graph with scatter points showed the quantification of iNOS in (A) . (H,I) Bar graphs with scatter points showed the quantification of IL-1β mRNA (H) and IL-6 mRNA (I) in the control group and P. gingivalis -infected mice injected with USP22-shRNA AAV. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: HBMECs were exposed to 100 ng/mL LPS from P. gingivalis (tlrl-pglps, InvivoGen) for 24 h.

Techniques: Knockdown, Immunofluorescence, Expressing, Control, Infection, Injection, shRNA