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Dendrogram of the PFGE- SmaI and PFGE- ApaI profiles of MRSA isolated from insulin-dependent diabetic individuals generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and their molecular characterization by SCC mec typing and MLST. a. Isolates showing > 80% similarity ( clusters A and B) after digestion with SmaI . b. Band pattern of strain 735 N obtained by digestion with ApaI . N, nasal mucosa; O, oropharyngeal mucosa; S, susceptible; R, resistant. * International clones used as controls

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

doi: 10.1186/s12941-020-00401-y

Figure Lengend Snippet: Dendrogram of the PFGE- SmaI and PFGE- ApaI profiles of MRSA isolated from insulin-dependent diabetic individuals generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and their molecular characterization by SCC mec typing and MLST. a. Isolates showing > 80% similarity ( clusters A and B) after digestion with SmaI . b. Band pattern of strain 735 N obtained by digestion with ApaI . N, nasal mucosa; O, oropharyngeal mucosa; S, susceptible; R, resistant. * International clones used as controls

Article Snippet: Fig. 3 Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST.

Techniques: Isolation, Generated, Clone Assay

Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST. Clustering of isolates digested with ApaI that were analyzed by MLST. All isolates except for 735 N were susceptible to methicillin (MSSA). N, nasal mucosa; O, oropharyngeal mucosa; arc C, carbamate kinase; aro E, shikimate dehydrogenase; glp F, glycerol kinase; gmk , guanylate kinase; pta , phosphate acetyltransferase; tpi , triosephosphate isomerase; yqi L, acetyl coenzyme A; ST, sequence type. Isolate 76 N was identified as ST398 in a previous study from our group. Isolate 700O was sent to the curator of the MLST database ( https://pubmlst.org/ ) for identification of the new ST. This isolate was identified as ST 6133

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

doi: 10.1186/s12941-020-00401-y

Figure Lengend Snippet: Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST. Clustering of isolates digested with ApaI that were analyzed by MLST. All isolates except for 735 N were susceptible to methicillin (MSSA). N, nasal mucosa; O, oropharyngeal mucosa; arc C, carbamate kinase; aro E, shikimate dehydrogenase; glp F, glycerol kinase; gmk , guanylate kinase; pta , phosphate acetyltransferase; tpi , triosephosphate isomerase; yqi L, acetyl coenzyme A; ST, sequence type. Isolate 76 N was identified as ST398 in a previous study from our group. Isolate 700O was sent to the curator of the MLST database ( https://pubmlst.org/ ) for identification of the new ST. This isolate was identified as ST 6133

Article Snippet: Fig. 3 Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST.

Techniques: Generated, Sequencing