Journal: iScience

Article Title: The lactate receptor HCAR1: A key modulator of epileptic seizure activity

doi: 10.1016/j.isci.2024.109679

Figure Lengend Snippet:

Article Snippet: A polyimide 30 channels EEG electrode array (H32, Neuronexus, Ann Arbor, MI, USA) was placed on the surface of the skull.

Techniques: Recombinant, Software

Journal: iScience

Article Title: The lactate receptor HCAR1: A key modulator of epileptic seizure activity

doi: 10.1016/j.isci.2024.109679

Figure Lengend Snippet:

Article Snippet: EEG electrode array , Neuronexus , Cat.# H32.

Techniques: Recombinant, Software

Journal: iScience

Article Title: The lactate receptor HCAR1: A key modulator of epileptic seizure activity

doi: 10.1016/j.isci.2024.109679

Figure Lengend Snippet: Spatial distribution of EEG power during seizures (A) Schematic representation of the EEG electrode array implant comprising 30 electrodes. (B) Example traces of 7 out of 30 channels. Detected seizure activity is highlighted in red in the traces. (C) Power of the different frequency bands for the average seizure in the WT and HCAR1-KO averaged over time and channels. Global (0-200Hz) : Means ± SEM: WT = 8.86 ± 1.47, HCAR1-KO = 13.67 ± 0.84, p = 0.0110. Delta (1-3Hz) : Means ± SEM: WT = 9.09 ± 3.70, HCAR1-KO = 19.85 ± 3.14, p = 0.0046. Theta (4-8Hz) : Means ± SEM: WT 9.540 ± 3.077, HCAR1-KO 21.44 ± 2.48, p = 0.0103. Alpha (9-13Hz) : Means ± SEM: WT = 8.88 ± 2.218, HCAR1-KO = 21.20 ± 2.104, p = 0.0019. Beta (14-30Hz) : Means ± SEM: WT = 9.005 ± 1.69, HCAR1-KO = 19.09 ± 1.62, p = 0.0012. Gamma (31-80Hz) : Means ± SEM: WT = 8.253 ± 1.313, HCAR1-KO = 14.12 ± 1.08 p = 0.004. HFO (80-200Hz) : Means ± SEM: WT = 9.07 ± 1.70, HCAR1-KO = 11.95 ± 0.80. p = 0.1228, unpaired t test, n = 6 WT, n = 8 HCAR1-KO. (D) Topographical maps of the average seizure in WT and HCAR1-KO mice showing the spread of the power through the brain according to frequency bands. Pseudocolors represent the power in dB of the frequency bands at each point in space. n = 6 WT, n = 8 HCAR1-KO.

Article Snippet: EEG electrode array , Neuronexus , Cat.# H32.

Techniques: Activity Assay

Journal: iScience

Article Title: The lactate receptor HCAR1: A key modulator of epileptic seizure activity

doi: 10.1016/j.isci.2024.109679

Figure Lengend Snippet: HCAR1 agonists fail to modulate seizures in the intrahippocampal kainate injection model (A) Schematic representation of subdural electrode placement over the dorsal hippocampus and the craniotomies for kainate injection. Left: top view. Right: Coronal view depicting the injection points as red circles. (B) Image of the 3Cl-HBA injection point in the dentate gyrus after the injection of Evan’s blue dye. (C) Example trace of an EEG recording displaying typical seizure events. (D–F) Power of the frequencies in the ipsilateral and contralateral hemispheres during seizures for (D) Global (0-500Hz): Means ± SEM: saline ipsilateral = 13.74 ± 0.67, saline contralateral = 16.58 ± 1.59, 3Cl-HBA ipsilateral = 15.13 ± 1.21, 3Cl-HBA contralateral = 15.93 ± 1.42. p > 0.005. (E) Theta (4-8Hz): Means ± SEM: saline ipsilateral = 12.83 ± 0.81, saline contralateral = 15.50 ± 1.53, 3Cl-HBA ipsilateral = 14.05 ± 1.24, 3Cl-HBA contralateral = 14.30 ± 1.42. p > 0.005. (F) HFO (80-500Hz): Means ± SEM: saline ipsilateral = 7.53 ± 1.40, saline contralateral = 11.94 ± 3.59, 3Cl-HBA ipsilateral = 10.25 ± 0.99, 3Cl-HBA contralateral = 9.17 ± 1.24. p > 0.005. Two-way ANOVA n = 9 control, n = 10 3Cl-HBA (2.5mM).

Article Snippet: EEG electrode array , Neuronexus , Cat.# H32.

Techniques: Injection, Saline, Control

Journal: iScience

Article Title: The lactate receptor HCAR1: A key modulator of epileptic seizure activity

doi: 10.1016/j.isci.2024.109679

Figure Lengend Snippet:

Article Snippet: EEG electrode array , Neuronexus , Cat.# H32.

Techniques: Recombinant, Software