Journal: Frontiers in Cell and Developmental Biology

Article Title: Perillaldehyde synergizes with ferroptosis inducers to promote ferroptotic cell death in gastric cancer

doi: 10.3389/fcell.2025.1598520

Figure Lengend Snippet: PAH inhibits the growth of gastric cancer cells in vitro (A) Chemical structure of perillaldehyde. (B,C) Human gastric cancer cell lines HGC27 and AGS were treated with the indicated concentrations of PAH for 24 or 48 h. Cell viability was assessed by MTT assay ( n = 4). (D) Mouse gastric cancer cell lines MFC were treated with different concentrations of PAH for 24 or 48 h. Cell viability was assessed by MTT assay ( n = 4). (E) Changes in cell morphology after PAH treatment of HGC27 and AGS cells. Scale bar, 100 μm. (F,H) Cell colony formation of HGC27, AGS and MFC cells treated with different concentrations of PAH, and the number of cells forming colonies was quantified using ImageJ software. (G) Calcein AM/PI fluorescence staining of HGC27, AGS and MFC cells treated with different concentrations of PAH, Calcein AM staining of live cells, showing green fluorescence; (PI) staining of dead cells, showing red fluorescence. Scale bar, 100 μm. (I,J) Cell migration of HGC27 and AGS cells treated with different concentrations of PAH for 24 h by wound healing assay, and the cell migration area was quantified by ImageJ to calculate the cell migration rate. Scale bar, 100 μm. (Data are expressed as mean ± SD, n = 4. ns, not statistically significant, * indicates compared with the control group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Article Snippet: Perillaldehyde (PAH, S3205, Selleck), dimethyl sulfoxide (DMSO, HY-Y0320, MCE), Z-VAD-FMK (S7023, Selleck), Necrostatin-1 (Nec-1; S8037, Selleck), Ferrostatin-1 (Fer-1; HY-100579, MCE), (1S, 3R)-RSL3 (RSL3; Y-100218A, MCE), Doxorubicin (DOX; T1020, TargetMol), Cisplatin (CIS; S1166, Selleck), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; M158055, Aladdin), crystal violet (C110703, Aladdin), 5-Fluorouracil (5-FU; HY-90006, MCE), 2′,7′-dichlorodihydrofluorescein diacetate (H 2 DCFDA; DCF; HY-D0940, MCE), dihydroethidium (DHE; 50102ES02, Yeasen Biotechnology), JC-1 mitochondrial membrane potential assay kit (JC-1; J6004S, UE Landy Biotechnology), Calcein/PI cell viability and cytotoxicity assay kit (C2015M, Beyotime Biotechnology), Hieff Trans® in vitro siRNA/miRNA Transfection Reagent (40806ES02, Yeasen Biotechnology), GSH and GSSG assay kit (S0053, Beyotime Biotechnology), divalent iron ion detection probe (FerroOrange; F374, Dojindo), and lipid peroxidation sensor (BODIPY 581/591 C11; D3861, Invitrogen).

Techniques: In Vitro, MTT Assay, Software, Fluorescence, Staining, Migration, Wound Healing Assay, Control

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Chemical composition of the ALEO fruit.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Chromatogram from GC/MS analysis of ALEO: 1: alpha-pinene; 2: beta-pinene; 3: 3-carene; 4: D-limonene; 5: 3-isopropylbenzaldehyde; 6: perillaldehyde; 7: methyl (2E)-(2isopropenylcyclopentylidene)ethanoate; 8: eudesma-4 (14), 11-diene.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: Gas Chromatography-Mass Spectrometry

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Chemical structure of perillaldehyde.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Mass spectrum obtained from GC/MS analysis of perillaldehyde.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: Gas Chromatography-Mass Spectrometry

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Effect of A. leucotrichus essential oil (ALEO), perillaldehyde (A) , and verapamil (B) on the basal tone of the rabbit jejunum. **, p < 0.01 and ***, p < 0.001. The disparity exhibits statistical significance within the control group (the zero line). Mean ± S.E.M; n = 5.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: Control

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: IC 50 values for relaxant effects of A. leucotrichus essential oil (ALEO), perillaldehyde, and verapamil on rabbit basic contractions of jejunal smooth muscle.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Original records of the myorelaxant impact of the A. leucotrichus essential oil (ALEO) (A) and perillaldehyde (B) on the basic jejunum rabbit contractions.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: A. leucotrichus essential oil (ALEO) and perillaldehyde effects on the jejunum contracted by KCl (25 mM) (A) and CCh (10 −6 M) (B) . **, p < 0.01 and ***, p < 0.001. The disparity exhibits statistical significance within the control group (the zero line). Mean ± S.E.M; n = 5.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: Control

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: IC 50 values for the antispasmodic activity of A. leucotrichus essential oil (ALEO) and perillaldehyde on the rat jejunum pre-contracted by CCh or KCl.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: Activity Assay

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: 3D and 2D interactions of perillaldehyde with L-type voltage-gated calcium (Ca v 1.1) channel active site (A, B) , human M 2 muscarinic acetylcholine receptor (C, D) , and M 3 muscarinic acetylcholine receptor (E, F) .

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Binding energies of perillaldehyde, D-limonene, and atropine with the active sites of the M 3 muscarinic acetylcholine receptor and the human M 2 muscarinic acetylcholine receptor.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: Binding Assay

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: Binding energies of perillaldehyde, D-limonene, and verapamil with the active sites of the L-type voltage-gated Ca 2+ (Ca v 1) channel.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: Binding Assay

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: In silico absorption prediction of perillaldehyde.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: In Silico, Solubility, Permeability

Journal: Frontiers in Chemistry

Article Title: Evaluation of the anti-spasmodic activity of essential oils of Ammodaucus leucotrichus fruits and its main chemical component “perillaldehyde” on intestinal smooth muscle contractions of rodents: ex vivo and in silico approaches

doi: 10.3389/fchem.2024.1465674

Figure Lengend Snippet: In Silico toxicity prediction of perillaldehyde.

Article Snippet: Perillaldehyde was obtained from Thermo Scientific, and DMSO was purchased from Prolabo.

Techniques: In Silico