Journal: PLoS ONE

Article Title: Prognostic Significance and Gene Expression Profiles of p53 Mutations in Microsatellite-Stable Stage III Colorectal Adenocarcinomas

doi: 10.1371/journal.pone.0030020

Figure Lengend Snippet: Validation of deferentially expressed genes by qNPA and qRT-PCR based on p53 status in Stage III-MSS CRCs.

Article Snippet: Tissue sections were then incubated with polyclonal antibodies for LPAR6, PDLIM3, PLAT, IQGAP3, and SLC6A8 (Santa Cruz Biotech Inc, CA) for TRIM29 (Abnova, Walnut, CA), or with monoclonal antibody (121 SLE mouse monoclonal) for FUT3 (Santa Cruz Biotech Inc).

Techniques:

Journal: PLoS ONE

Article Title: Prognostic Significance and Gene Expression Profiles of p53 Mutations in Microsatellite-Stable Stage III Colorectal Adenocarcinomas

doi: 10.1371/journal.pone.0030020

Figure Lengend Snippet: a , normal colonic epithelium (NCE) demonstrating moderate cytoplasmic (thick arrow) and weak membrane (thin arrow) FUT3 staining (400 µm). b , CRCs exhibiting strong cytoplasmic (thick arrows) and moderate to weak membrane FUT3 staining (thin arrows) (400 µm). c , CRCs with weak cytoplasmic staining (thin arrows) (400 µm). d , NCE demonstrating weak cytoplasmic TRIM29 staining with a focal punctuate pattern (thin arrows) (400 µm). e , CRCs exhibiting moderate to strong cytoplasmic TRIM29 staining with a punctate pattern on the luminal aspect (thin arrows) (600 µm). f , CRCs with weak cytoplasmic TRIM29 staining (thin arrows) (400 µm). g , NCE demonstrating weak cytoplasmic to complete lack of IQGAP3 staining. [Note: Lymphocytes in the stroma show moderate cytoplasmic staining (thin arrows); the adjacent tumor demonstrates moderate cytoplasmic and nuclear immunostaining (thick arrows) (400 µm)]. h , CRCs exhibiting strong cytoplasmic IQGAP3 staining (thick arrows) (400 µm). i , CRCs with lack of staining for IQGAP3 (thick arrows) (400 µm). j , NCE demonstrating moderate cytoplasmic staining of SLC6A8 staining (thin arrows) (600 µm). k , CRCs exhibiting strong cytoplasmic SLC6A8 staining with luminal accentuation (thick arrows) (600 µm). l , CRCs negative for SLC6A8 staining (thick arrows) (600 µm).

Article Snippet: Tissue sections were then incubated with polyclonal antibodies for LPAR6, PDLIM3, PLAT, IQGAP3, and SLC6A8 (Santa Cruz Biotech Inc, CA) for TRIM29 (Abnova, Walnut, CA), or with monoclonal antibody (121 SLE mouse monoclonal) for FUT3 (Santa Cruz Biotech Inc).

Techniques: Staining, Immunostaining