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Figure 6. HCA inhibited the <t>proliferation</t> and migration of PASMCs induced by hypoxia. A: The <t>PASMC</t> proliferation activity detected by CCK-8. B: Representative images of PASMCs proliferation measured using EdU. C: EdU quantification. D: Representative images of the cell scratch test. E: Quantification of the cell scratch test (%, cell scratch area ratio). HCA indicates hydroxycitric acid tripotassium hydrate; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns, P > 0.05.
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Figure 6. HCA inhibited the <t>proliferation</t> and migration of PASMCs induced by hypoxia. A: The <t>PASMC</t> proliferation activity detected by CCK-8. B: Representative images of PASMCs proliferation measured using EdU. C: EdU quantification. D: Representative images of the cell scratch test. E: Quantification of the cell scratch test (%, cell scratch area ratio). HCA indicates hydroxycitric acid tripotassium hydrate; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns, P > 0.05.
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Figure 6. HCA inhibited the <t>proliferation</t> and migration of PASMCs induced by hypoxia. A: The <t>PASMC</t> proliferation activity detected by CCK-8. B: Representative images of PASMCs proliferation measured using EdU. C: EdU quantification. D: Representative images of the cell scratch test. E: Quantification of the cell scratch test (%, cell scratch area ratio). HCA indicates hydroxycitric acid tripotassium hydrate; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns, P > 0.05.
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Figure 6. HCA inhibited the <t>proliferation</t> and migration of PASMCs induced by hypoxia. A: The <t>PASMC</t> proliferation activity detected by CCK-8. B: Representative images of PASMCs proliferation measured using EdU. C: EdU quantification. D: Representative images of the cell scratch test. E: Quantification of the cell scratch test (%, cell scratch area ratio). HCA indicates hydroxycitric acid tripotassium hydrate; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns, P > 0.05.
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Figure 6. HCA inhibited the proliferation and migration of PASMCs induced by hypoxia. A: The PASMC proliferation activity detected by CCK-8. B: Representative images of PASMCs proliferation measured using EdU. C: EdU quantification. D: Representative images of the cell scratch test. E: Quantification of the cell scratch test (%, cell scratch area ratio). HCA indicates hydroxycitric acid tripotassium hydrate; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns, P > 0.05.

Journal: International heart journal

Article Title: Hydroxycitric Acid Tripotassium Hydrate Attenuates Monocrotaline and Hypoxia-Induced Pulmonary Hypertension in Rats.

doi: 10.1536/ihj.23-350

Figure Lengend Snippet: Figure 6. HCA inhibited the proliferation and migration of PASMCs induced by hypoxia. A: The PASMC proliferation activity detected by CCK-8. B: Representative images of PASMCs proliferation measured using EdU. C: EdU quantification. D: Representative images of the cell scratch test. E: Quantification of the cell scratch test (%, cell scratch area ratio). HCA indicates hydroxycitric acid tripotassium hydrate; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns, P > 0.05.

Article Snippet: Cell proliferation assay: PASMC proliferation was examined using the Cell Counting Kit-8 (Beyotime, China) assay and the EdU Cell Proliferation Kit with Alexa Fluor 488 (Beyotime, China) assay following the manufacturer’s instructions.

Techniques: Migration, Activity Assay, CCK-8 Assay