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long-wd air parfocal objectives 2  (Mitutoyo)
90
Mitutoyo long-wd air parfocal objectives 2
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
Long Wd Air Parfocal Objectives 2, supplied by Mitutoyo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/pmc10973490-99-26-4?v=Mitutoyo
Average 90 stars, based on 1 article reviews
long-wd air parfocal objectives 2 - by Bioz Stars, 2026-07
90/100 stars
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long-wd air parfocal objectives 2x  (Mitutoyo)
90
Mitutoyo long-wd air parfocal objectives 2x
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
Long Wd Air Parfocal Objectives 2x, supplied by Mitutoyo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/pmc10760166-298-26-4?v=Mitutoyo
Average 90 stars, based on 1 article reviews
long-wd air parfocal objectives 2x - by Bioz Stars, 2026-07
90/100 stars
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objective w plan-apochromat 20×/1.0 dic m27 parfocal length (free working distance =)  (Carl Zeiss)
90
Carl Zeiss objective w plan-apochromat 20×/1.0 dic m27 parfocal length (free working distance =)
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
Objective W Plan Apochromat 20×/1.0 Dic M27 Parfocal Length (Free Working Distance =), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/pm37857852-246-9-21?v=Carl+Zeiss
Average 90 stars, based on 1 article reviews
objective w plan-apochromat 20×/1.0 dic m27 parfocal length (free working distance =) - by Bioz Stars, 2026-07
90/100 stars
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parfocal beam splitter optosplit ii bypass  (Cairn Research Ltd)
90
Cairn Research Ltd parfocal beam splitter optosplit ii bypass
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
Parfocal Beam Splitter Optosplit Ii Bypass, supplied by Cairn Research Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/pm36561216-92-1-7?v=Cairn+Research+Ltd
Average 90 stars, based on 1 article reviews
parfocal beam splitter optosplit ii bypass - by Bioz Stars, 2026-07
90/100 stars
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motorised parfocal zoom lens  (Navistar Inc)
90
Navistar Inc motorised parfocal zoom lens
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
Motorised Parfocal Zoom Lens, supplied by Navistar Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/10__1364_slash_oe__459771-84-5-4?v=Navistar+Inc
Average 90 stars, based on 1 article reviews
motorised parfocal zoom lens - by Bioz Stars, 2026-07
90/100 stars
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parfocal lengths  (Nikon)
99
Nikon parfocal lengths
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
Parfocal Lengths, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/bio_rxiv__2020__05__06__081745-160-2-15?v=Nikon
Average 99 stars, based on 1 article reviews
parfocal lengths - by Bioz Stars, 2026-07
99/100 stars
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15mm parfocal extender  (Thorlabs)
90
Thorlabs 15mm parfocal extender
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
15mm Parfocal Extender, supplied by Thorlabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/pm32888483-235-32-35?v=Thorlabs
Average 90 stars, based on 1 article reviews
15mm parfocal extender - by Bioz Stars, 2026-07
90/100 stars
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oca15plus 6x parfocal zoom lens typed contact-angle tester  (DataPhysics Instruments)
90
DataPhysics Instruments oca15plus 6x parfocal zoom lens typed contact-angle tester
a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between <t>mesoSPIM</t> v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.
Oca15plus 6x Parfocal Zoom Lens Typed Contact Angle Tester, supplied by DataPhysics Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parfocal+system/10__1016_slash_j__surfin__2021__101172-98-20-12?v=DataPhysics+Instruments
Average 90 stars, based on 1 article reviews
oca15plus 6x parfocal zoom lens typed contact-angle tester - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between mesoSPIM v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between mesoSPIM v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: Microscopy, Labeling, Modification, Comparison, Imaging

a The testing procedure of the detection objective contrast properties includes taking a focus stack of the Ronchi ruling immersed into the imaging medium, splitting each image into sub-regions, and calculating the local contrast of each sub-region. b Example of a 3D contrast map for objective Olympus XLFluor 4×/0.28. c Comparison of absolute contrast and field flatness across the field between Benchtop mesoSPIM (Mitutoyo Plan Apo BD 5×/0.14) vs. mesoSPIM v5 (Olympus MVPLAPO-1×, at zoom 5×). Sensor dimensions and colormap scales are equal.

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: a The testing procedure of the detection objective contrast properties includes taking a focus stack of the Ronchi ruling immersed into the imaging medium, splitting each image into sub-regions, and calculating the local contrast of each sub-region. b Example of a 3D contrast map for objective Olympus XLFluor 4×/0.28. c Comparison of absolute contrast and field flatness across the field between Benchtop mesoSPIM (Mitutoyo Plan Apo BD 5×/0.14) vs. mesoSPIM v5 (Olympus MVPLAPO-1×, at zoom 5×). Sensor dimensions and colormap scales are equal.

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: Imaging, Comparison

The Benchtop allows larger overall FOV and higher contrast across the image. The mesoSPIM v5 system has areas of lower resolution (ROI# 1 ) and higher resolution (ROI# 2 ) depending on the region’s position, consistent with the maps of objective contrast shown in Fig. . MIPs over multiple z-planes are shown. The original grayscale 16-bit map was pseudo-colored with a yellow LUT. Each imaging experiment was performed twice with nearly identical results.

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: The Benchtop allows larger overall FOV and higher contrast across the image. The mesoSPIM v5 system has areas of lower resolution (ROI# 1 ) and higher resolution (ROI# 2 ) depending on the region’s position, consistent with the maps of objective contrast shown in Fig. . MIPs over multiple z-planes are shown. The original grayscale 16-bit map was pseudo-colored with a yellow LUT. Each imaging experiment was performed twice with nearly identical results.

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: Imaging

mesoSPIM v.5 vs Benchtop comparison

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: mesoSPIM v.5 vs Benchtop comparison

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: