Journal: PLoS ONE

Article Title: Plasma-derived exosome-like vesicles are enriched in lyso-phospholipids and pass the blood-brain barrier

doi: 10.1371/journal.pone.0232442

Figure Lengend Snippet: EVs originating from human plasma was isolated by SEC using qEV columns (iZON). Plasma (P) and EVs were separated by Bolt ready made 10% gels at denaturing conditions and stained with CBB (A). Proteins were transferred to a NC membrane and incubated in 1° Ab CD63, CD9 and Hsp70 produced in rabbit (1:1000) and 2° Ab Goat anti-Rabbit HRP (1:20000) (B). To determine the protein to vesicle ratio absorbance at 280 nm (grey) and 498 nm (red) were determined using a SpectraMax® Paradigm® Multi-Mode Microplate reader (Molecular devices) (C). The size and poly-dispersity of the isolated vesicles were analyzed in a Zetasizer Nano ZSP instrument (Malvern, UK) (D).

Article Snippet: The correlation of protein and lipids in plasma and isolated EVs were determined by absorbance spectroscopy at 280 nm ( , grey bar) and 495 nm ( , red bar) in a SpectraMax® Paradigm® Multi-Mode Micro-plate reader (Molecular devices).

Techniques: Isolation, Staining, Incubation, Produced