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Main histopathological and immunohistochemical findings in the spleen, kidney and diaphragm. (A) Spleen. Complete loss of the normal splenic histology, replaced by a large number of pleomorphic cells with morphology consistent with macrophages. Hematoxylin and eosin (HE), x400; (B) Spleen. Vascular invasion of neoplastic cells into medium‐calibre blood vessels (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x200; (C) Spleen. Higher‐magnification image of another vascular invasion (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x400; (D) Kidney. Highly pleomorphic cells (arrowheads) in the renal parenchyma suggestive of metastasis from the histiocytic sarcoma, associated with a marked neutrophilic inflammatory infiltrate, HE, x400; (E) Kidney. Intense cytoplasmatic positive immunoreaction for Iba‐1 in the histiocytic sarcoma metastases of the renal parenchyma, x400; (F) Kidney. Negative immunoreactivity of the renal histiocytic sarcoma metastasis (arrowhead) for pancytokeratin <t>AE1/AE3,</t> ruling out an epithelial origin, pancytokeratin AE1/AE3, x200; (G) Diaphragm. Metastatic nodule of histiocytic sarcoma in the diaphragm (arrowhead), HE, x40. Inset: higher‐magnification image of neoplastic cells infiltrating the diaphragm, HE, x400; (H) Diaphragm. Strong positive immunoreactivity of the metastatic nodule (arrowhead) shown in Figure for Iba‐1, x40. Inset: higher‐magnification image of the metastatic nodule, Iba‐1, x400.
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Main histopathological and immunohistochemical findings in the spleen, kidney and diaphragm. (A) Spleen. Complete loss of the normal splenic histology, replaced by a large number of pleomorphic cells with morphology consistent with macrophages. Hematoxylin and eosin (HE), x400; (B) Spleen. Vascular invasion of neoplastic cells into medium‐calibre blood vessels (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x200; (C) Spleen. Higher‐magnification image of another vascular invasion (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x400; (D) Kidney. Highly pleomorphic cells (arrowheads) in the renal parenchyma suggestive of metastasis from the histiocytic sarcoma, associated with a marked neutrophilic inflammatory infiltrate, HE, x400; (E) Kidney. Intense cytoplasmatic positive immunoreaction for Iba‐1 in the histiocytic sarcoma metastases of the renal parenchyma, x400; (F) Kidney. Negative immunoreactivity of the renal histiocytic sarcoma metastasis (arrowhead) for pancytokeratin <t>AE1/AE3,</t> ruling out an epithelial origin, pancytokeratin AE1/AE3, x200; (G) Diaphragm. Metastatic nodule of histiocytic sarcoma in the diaphragm (arrowhead), HE, x40. Inset: higher‐magnification image of neoplastic cells infiltrating the diaphragm, HE, x400; (H) Diaphragm. Strong positive immunoreactivity of the metastatic nodule (arrowhead) shown in Figure for Iba‐1, x40. Inset: higher‐magnification image of the metastatic nodule, Iba‐1, x400.
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Main histopathological and immunohistochemical findings in the spleen, kidney and diaphragm. (A) Spleen. Complete loss of the normal splenic histology, replaced by a large number of pleomorphic cells with morphology consistent with macrophages. Hematoxylin and eosin (HE), x400; (B) Spleen. Vascular invasion of neoplastic cells into medium‐calibre blood vessels (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x200; (C) Spleen. Higher‐magnification image of another vascular invasion (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x400; (D) Kidney. Highly pleomorphic cells (arrowheads) in the renal parenchyma suggestive of metastasis from the histiocytic sarcoma, associated with a marked neutrophilic inflammatory infiltrate, HE, x400; (E) Kidney. Intense cytoplasmatic positive immunoreaction for Iba‐1 in the histiocytic sarcoma metastases of the renal parenchyma, x400; (F) Kidney. Negative immunoreactivity of the renal histiocytic sarcoma metastasis (arrowhead) for pancytokeratin <t>AE1/AE3,</t> ruling out an epithelial origin, pancytokeratin AE1/AE3, x200; (G) Diaphragm. Metastatic nodule of histiocytic sarcoma in the diaphragm (arrowhead), HE, x40. Inset: higher‐magnification image of neoplastic cells infiltrating the diaphragm, HE, x400; (H) Diaphragm. Strong positive immunoreactivity of the metastatic nodule (arrowhead) shown in Figure for Iba‐1, x40. Inset: higher‐magnification image of the metastatic nodule, Iba‐1, x400.
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Main histopathological and immunohistochemical findings in the spleen, kidney and diaphragm. (A) Spleen. Complete loss of the normal splenic histology, replaced by a large number of pleomorphic cells with morphology consistent with macrophages. Hematoxylin and eosin (HE), x400; (B) Spleen. Vascular invasion of neoplastic cells into medium‐calibre blood vessels (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x200; (C) Spleen. Higher‐magnification image of another vascular invasion (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x400; (D) Kidney. Highly pleomorphic cells (arrowheads) in the renal parenchyma suggestive of metastasis from the histiocytic sarcoma, associated with a marked neutrophilic inflammatory infiltrate, HE, x400; (E) Kidney. Intense cytoplasmatic positive immunoreaction for Iba‐1 in the histiocytic sarcoma metastases of the renal parenchyma, x400; (F) Kidney. Negative immunoreactivity of the renal histiocytic sarcoma metastasis (arrowhead) for pancytokeratin <t>AE1/AE3,</t> ruling out an epithelial origin, pancytokeratin AE1/AE3, x200; (G) Diaphragm. Metastatic nodule of histiocytic sarcoma in the diaphragm (arrowhead), HE, x40. Inset: higher‐magnification image of neoplastic cells infiltrating the diaphragm, HE, x400; (H) Diaphragm. Strong positive immunoreactivity of the metastatic nodule (arrowhead) shown in Figure for Iba‐1, x40. Inset: higher‐magnification image of the metastatic nodule, Iba‐1, x400.
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Main histopathological and immunohistochemical findings in the spleen, kidney and diaphragm. (A) Spleen. Complete loss of the normal splenic histology, replaced by a large number of pleomorphic cells with morphology consistent with macrophages. Hematoxylin and eosin (HE), x400; (B) Spleen. Vascular invasion of neoplastic cells into medium‐calibre blood vessels (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x200; (C) Spleen. Higher‐magnification image of another vascular invasion (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x400; (D) Kidney. Highly pleomorphic cells (arrowheads) in the renal parenchyma suggestive of metastasis from the histiocytic sarcoma, associated with a marked neutrophilic inflammatory infiltrate, HE, x400; (E) Kidney. Intense cytoplasmatic positive immunoreaction for Iba‐1 in the histiocytic sarcoma metastases of the renal parenchyma, x400; (F) Kidney. Negative immunoreactivity of the renal histiocytic sarcoma metastasis (arrowhead) for pancytokeratin AE1/AE3, ruling out an epithelial origin, pancytokeratin AE1/AE3, x200; (G) Diaphragm. Metastatic nodule of histiocytic sarcoma in the diaphragm (arrowhead), HE, x40. Inset: higher‐magnification image of neoplastic cells infiltrating the diaphragm, HE, x400; (H) Diaphragm. Strong positive immunoreactivity of the metastatic nodule (arrowhead) shown in Figure for Iba‐1, x40. Inset: higher‐magnification image of the metastatic nodule, Iba‐1, x400.

Journal: Veterinary Medicine and Science

Article Title: Histological and Immunohistochemical Characterization of a Histiocytic Sarcoma Associated With a Follicular Lymphoma in a Ring‐tailed Lemur ( Lemur catta )

doi: 10.1002/vms3.70800

Figure Lengend Snippet: Main histopathological and immunohistochemical findings in the spleen, kidney and diaphragm. (A) Spleen. Complete loss of the normal splenic histology, replaced by a large number of pleomorphic cells with morphology consistent with macrophages. Hematoxylin and eosin (HE), x400; (B) Spleen. Vascular invasion of neoplastic cells into medium‐calibre blood vessels (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x200; (C) Spleen. Higher‐magnification image of another vascular invasion (arrowhead) within the splenic nodule showing strong immunoreactivity for Iba‐1, x400; (D) Kidney. Highly pleomorphic cells (arrowheads) in the renal parenchyma suggestive of metastasis from the histiocytic sarcoma, associated with a marked neutrophilic inflammatory infiltrate, HE, x400; (E) Kidney. Intense cytoplasmatic positive immunoreaction for Iba‐1 in the histiocytic sarcoma metastases of the renal parenchyma, x400; (F) Kidney. Negative immunoreactivity of the renal histiocytic sarcoma metastasis (arrowhead) for pancytokeratin AE1/AE3, ruling out an epithelial origin, pancytokeratin AE1/AE3, x200; (G) Diaphragm. Metastatic nodule of histiocytic sarcoma in the diaphragm (arrowhead), HE, x40. Inset: higher‐magnification image of neoplastic cells infiltrating the diaphragm, HE, x400; (H) Diaphragm. Strong positive immunoreactivity of the metastatic nodule (arrowhead) shown in Figure for Iba‐1, x40. Inset: higher‐magnification image of the metastatic nodule, Iba‐1, x400.

Article Snippet: For lineage evaluation and cellular characterization, a comprehensive immunohistochemical panel (indirect immunohistochemical technique with endogenous peroxidase blocking and chromogenic detection) was performed, consisting of Ionized calcium‐binding adaptor molecule 1 (Iba‐1; Polyclonal Rabbit Anti‐Human, Thermo Fisher 1:100), PAX‐5‐BSAP (B‐cell‐specific activator protein; Monoclonal Mouse Anti‐Human, Dako, Ready To Use (RTU)), CD3 (Polyclonal Rabbit Anti‐Human, Dako, RTU), IRF‐4 (Interferon Regulatory Factor 4; Monoclonal Rabbit Anti‐Human, Abcam, 1:100) and Pancytokeratin AE1/AE3 (Monoclonal Mouse Anti‐Human, Vitro Master Diagnostica, RTU), aimed at excluding a potential primary renal epithelial neoplasm.

Techniques: Immunohistochemical staining