Journal: Animals : an Open Access Journal from MDPI

Article Title: Effects of an Hourly Bolus Postruminal Infusion of Flaxseed Oil or Palm Oil on Circulating Fatty Acid Concentrations and Hepatic Expression of Pyruvate Carboxylase and Phosphoenolpyruvate Carboxykinase in Dairy Cattle

doi: 10.3390/ani13223572

Figure Lengend Snippet: Diet ingredients and nutrient composition presented as averages or averages ± standard deviation 1 .

Article Snippet: The lack of response of the plasma fatty acid profile to palm oil in these experiments may have been the result of the inclusion of Palmit 80 (Global Agri-trade Corporation, Rancho Dominguez, CA) in the basal diet a dry, a beaded ingredient consisting of 85% palmitic acid (C16:0).

Techniques: Standard Deviation

Journal: iScience

Article Title: Super-multiplex imaging of cellular dynamics and heterogeneity by integrated stimulated Raman and fluorescence microscopy

doi: 10.1016/j.isci.2021.102832

Figure Lengend Snippet: Super-multiplex imaging of biological specimens covering diverse molecular contrasts (A) 8-color organelle imaging of live HeLa cells labeled by eight kinds of organelle-targeted Raman and fluorescent probes. SRS: mitochondria (Mito), lysosomes (Lyso), lipid droplets (LD), ER fluorescence: nucleus, plasma membrane (PM), tubulin, actin. The multiplexed images acquired within 30 s display cells undergoing cytokinesis as well as the distribution of all eight kinds of organelles. The arrow indicates the position of midbody. (B) 8-color imaging of live HeLa cells including label-free SRS imaging. SRS: protein, lipid; fluorescence: nucleus, Mito, PM, Golgi apparatus, tubulin, actin. The total acquisition time was 2 s. Inset shows a magnified view of the area indicated by the dashed box. The arrows indicate the positions of nucleoli. (C) 7-color depth-resolved imaging of fixed brain tissue slice from CAG-EGFP mouse including label-free SRS imaging. SRS: protein, lipid, blood vessel; fluorescence: nucleus, EGFP driven by a ubiquitous CAG promoter, glial fibrillary acidic protein (GFAP), actin. The acquisition time of seven images at each depth was 16 s, i.e. 80 s for all five depths. (D) 7-color pulse-chase imaging of live HeLa cells including metabolic imaging. SRS: metabolites of palmitic acid-d 31 (PA) and arachidonic acid-d 8 (AA); fluorescence: nucleus, Mito, PM, Lyso, actin. The total acquisition time of every seven images was 2 s. The images of AA/PA ratio were calculated from the metabolite images of PA and AA. The contours of nucleus and PM were depicted with solid lines. Scale bars, 10 μm. (See also Figure S2 and ).

Article Snippet: 160 μM arachidonic acid-d8 (AA-d 8 ) (Cayman Chemical, 390010) and 80 μM palmitic acid-d 31 (PA-d 31 ) (Wako Pure Chemical Industries, 16497) was added in the culture media for 18 hours at 37°C.

Techniques: Multiplex Assay, Imaging, Labeling, Fluorescence, Pulse Chase