Journal: Animal Nutrition

Article Title: Modulating starch digestion kinetics via feed processing: Implications for growth and metabolism in weaned pigs

doi: 10.1016/j.aninu.2025.08.011

Figure Lengend Snippet: Effect of corn processing on relative mRNA level for mTOR , 4E-BP1 , p70S6K and protein abundances of mTOR, phosphorylated mTOR (p-mTOR), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), phosphorylated 4E-BP1 (p-4E-BP1), p70 ribosomal protein S6 kinase (p70S6K) and phosphorylated p70S6K (p-p70S6K) in the longissimus thoracis of piglets. Results are presented as means ± standard error of the mean (SEM), n = 4. Data columns with different letters were significantly different ( P ≤ 0.05).

Article Snippet: The membranes were blocked at room temperature, followed by incubation at 4 °C overnight with the following primary antibodies: mammalian target of rapamycin (mTOR, catalog No. 2983, Cell Signaling Technology, Danvers, MA, USA), phosphorylated mTOR (p-mTOR, catalog No. 5536, Cell Signaling Technology, Danvers, MA, USA), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1, catalog No. 9644, Cell Signaling Technology, Danvers, MA, USA), phosphorylated 4E-BP1 (p-4E-BP1, catalog No. 2855, Cell Signaling Technology, Danvers, MA, USA), p70 ribosomal protein S6 kinase (p70S6K, catalog No. 2708, Cell Signaling Technology, Danvers, MA, USA), phosphorylated p70S6K (p-p70S6K, catalog No. 9234, Cell Signaling Technology, Danvers, MA, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, catalog No. 5174, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Binding Assay

Journal: bioRxiv

Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

doi: 10.64898/2026.03.31.715667

Figure Lengend Snippet: (A-G) mTOR signaling in the liver was determined by Western blot analysis of the following phosphorylated and unphosphorylated proteins; S6 (B), 4e-BP1 (C), S6K1 (D), eiF2α (E), AKT (F-G), HSP90 (loading control). (H-J) mTORC1 signaling in the muscle was determined by Western blot analysis of phosphorylated S6 (I), phosphorylated 4e-BP1 (K). HSP90 was used as a loading control. (B-G, I-J) Quantification was determined by normalizing phosphorylated protein to normal protein. (A-J) n=5-6 mice/group, quantification was determined using ImageJ by normalizing the phosphorylated protein to the unphosphorylated form. Three-way ANOVA between sex, diet, and gonadectomy with post hoc Šidák’s adjusted test for pairwise comparisons, *p<0.05. p values for the overall effect of sex, diet, and gonadectomy and the interactions represent the significant p values from the three-way ANOVA. Data are represented as mean ±SEM. Abbreviations: C, CTL (21% control protein), L, LP (7% low protein), Cast (castration), Fem (female), Ovx (ovariectomy), Gonad (gonadectomy). Created in BioRender. Knopf, B. (2026) https://BioRender.com/i0gripn

Article Snippet: Primary antibodies were used at 1:1,000 and were purchased from Cell Signaling Technology (Danvers, MA, USA): p-T389 p70 S6K1 (#9205), p70 S6K1 (#2708), p-Ser240/244 S6 (#2215), S6 (#2217), p-Thr37/46 4E-BP1(#2855), 4E-BP1 (#9452), p-S51 eiF2α (#3398), eiF2α (#5324), p- S473 AKT (#4060), p-T308 AKT (#9275), AKT (#4691), HSP90 (#4877).

Techniques: Western Blot, Control