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Effect of BBR intervention on protein expression in DKD rats by Western blot. ( A and B ) Western blot analysis and quantification of proteins in the <t>IL-17A/TRAF6/MAPK14</t> pathway in kidney tissues. *P < 0.05, # P > 0.05 vs the model group.
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Effect of BBR intervention on protein expression in DKD rats by Western blot. ( A and B ) Western blot analysis and quantification of proteins in the <t>IL-17A/TRAF6/MAPK14</t> pathway in kidney tissues. *P < 0.05, # P > 0.05 vs the model group.
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Effect of BBR intervention on protein expression in DKD rats by Western blot. ( A and B ) Western blot analysis and quantification of proteins in the <t>IL-17A/TRAF6/MAPK14</t> pathway in kidney tissues. *P < 0.05, # P > 0.05 vs the model group.
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Effect of BBR intervention on protein expression in DKD rats by Western blot. ( A and B ) Western blot analysis and quantification of proteins in the IL-17A/TRAF6/MAPK14 pathway in kidney tissues. *P < 0.05, # P > 0.05 vs the model group.

Journal: Journal of Inflammation Research

Article Title: Berberine Ameliorates Diabetic Kidney Disease by Modulating Macrophage Polarization via Inhibiting IL-17A Signaling

doi: 10.2147/JIR.S580534

Figure Lengend Snippet: Effect of BBR intervention on protein expression in DKD rats by Western blot. ( A and B ) Western blot analysis and quantification of proteins in the IL-17A/TRAF6/MAPK14 pathway in kidney tissues. *P < 0.05, # P > 0.05 vs the model group.

Article Snippet: These membranes were subsequently incubated overnight at 4°C with primary antibodies specifically targeting IL17A (Proteintech, 26163-1-AP, 1:3000), MCP-1 (Proteintech, 26161-1-AP, 1:2000), MMP9 (Proteintech, 10375-2-AP, 1:3000), TRAF6 (ABclonal, A23385, 1:10,000), MAPK14 (Proteintech, 14064-1-AP, 1:10,000), p-MAPK14 (Proteintech, 28796-1-AP, 1:3000), Caspase-3 (Proteintech, 19677-1-AP, 1:3000), Bcl-2 (Proteintech, 26593-1-AP, 1:3000), and Bax (Proteintech, 50599-2-lg, 1:10,000).

Techniques: Expressing, Western Blot