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(A): Oxygen consumption rates in isolated mitochondria from muscles of WT and MCK-SIRT3M3 mice at 5 months of age (n = 6). Respiration parameters were recorded using an Oroboros O2k <t>oxygraph.</t> Resting respiration (state 4) and maximal ADP-stimulated respiration (state 3) were presented. (B): Respiratory control ratio (RCR) was calculated as the ratio of oxygen consumption at state 3 over oxygen consumption at state 4. (C): Citrate synthase (CS) activity in gastrocnemius muscle extracts from WT and MCK-SIRT3 transgenic mice at 5 months of age (n = 6). CS activity was measured according to Srere . ** P <0.01 between WT and MCK-SIRT3M3 mice.
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(A): Oxygen consumption rates in isolated mitochondria from muscles of WT and MCK-SIRT3M3 mice at 5 months of age (n = 6). Respiration parameters were recorded using an Oroboros O2k oxygraph. Resting respiration (state 4) and maximal ADP-stimulated respiration (state 3) were presented. (B): Respiratory control ratio (RCR) was calculated as the ratio of oxygen consumption at state 3 over oxygen consumption at state 4. (C): Citrate synthase (CS) activity in gastrocnemius muscle extracts from WT and MCK-SIRT3 transgenic mice at 5 months of age (n = 6). CS activity was measured according to Srere . ** P <0.01 between WT and MCK-SIRT3M3 mice.

Journal: PLoS ONE

Article Title: Regulation of Skeletal Muscle Oxidative Capacity and Muscle Mass by SIRT3

doi: 10.1371/journal.pone.0085636

Figure Lengend Snippet: (A): Oxygen consumption rates in isolated mitochondria from muscles of WT and MCK-SIRT3M3 mice at 5 months of age (n = 6). Respiration parameters were recorded using an Oroboros O2k oxygraph. Resting respiration (state 4) and maximal ADP-stimulated respiration (state 3) were presented. (B): Respiratory control ratio (RCR) was calculated as the ratio of oxygen consumption at state 3 over oxygen consumption at state 4. (C): Citrate synthase (CS) activity in gastrocnemius muscle extracts from WT and MCK-SIRT3 transgenic mice at 5 months of age (n = 6). CS activity was measured according to Srere . ** P <0.01 between WT and MCK-SIRT3M3 mice.

Article Snippet: Data acquisition and analysis were performed using Oxygraph-2k-DatLab software version 4.3 (OROBOROS Instruments, Innsbruck, Austria).

Techniques: Isolation, Activity Assay, Transgenic Assay