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Analysis of oxidative DNA damage (8-oxo-dG) and <t>OGG1</t> enzyme expression after temperature stress and TBSV infection in N. benthamiana leaves. ( A ) 8-oxo-dG level, OD (n = 5, mean ± SD). Different capital letters indicate statistically significant differences between groups at p < 0.05 (ANOVA with Tukey’s multiple comparison test). ( B ) Western blot analysis to visualize OGG1 protein abundance and relative intensity (%) competed to control (numbers below the bands). This figure displays one representative experiment from two independent Western blot analyses. (-) TBSV—mock-inoculated; (+) TBSV—TBSV infected. Western blot original images can be found in .
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Relative fold change of genes expression involved in DNA repair processes: <t>Ogg1,</t> Parp1 , and Xrcc1 in PBMCs pretreated with 0.25, 0.5, 1 and 2 mg/mL of Gentiana lutea root extract and exposed to 0.5 and 2 Gy irradiation dose
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Image Search Results


Analysis of oxidative DNA damage (8-oxo-dG) and OGG1 enzyme expression after temperature stress and TBSV infection in N. benthamiana leaves. ( A ) 8-oxo-dG level, OD (n = 5, mean ± SD). Different capital letters indicate statistically significant differences between groups at p < 0.05 (ANOVA with Tukey’s multiple comparison test). ( B ) Western blot analysis to visualize OGG1 protein abundance and relative intensity (%) competed to control (numbers below the bands). This figure displays one representative experiment from two independent Western blot analyses. (-) TBSV—mock-inoculated; (+) TBSV—TBSV infected. Western blot original images can be found in .

Journal: Biomolecules

Article Title: TBSV Alters Host Redox State After Short-Term Temperature Pre-Exposure in Nicotiana benthamiana

doi: 10.3390/biom16030446

Figure Lengend Snippet: Analysis of oxidative DNA damage (8-oxo-dG) and OGG1 enzyme expression after temperature stress and TBSV infection in N. benthamiana leaves. ( A ) 8-oxo-dG level, OD (n = 5, mean ± SD). Different capital letters indicate statistically significant differences between groups at p < 0.05 (ANOVA with Tukey’s multiple comparison test). ( B ) Western blot analysis to visualize OGG1 protein abundance and relative intensity (%) competed to control (numbers below the bands). This figure displays one representative experiment from two independent Western blot analyses. (-) TBSV—mock-inoculated; (+) TBSV—TBSV infected. Western blot original images can be found in .

Article Snippet: OGG1 was detected using anti-OGG1 polyclonal antibodies (Cusabio, Houston, TX, USA) according to the manufacturer’s recommendations [ ].

Techniques: Expressing, Infection, Comparison, Western Blot, Quantitative Proteomics, Control

Relative fold change of genes expression involved in DNA repair processes: Ogg1, Parp1 , and Xrcc1 in PBMCs pretreated with 0.25, 0.5, 1 and 2 mg/mL of Gentiana lutea root extract and exposed to 0.5 and 2 Gy irradiation dose

Journal: Dose-Response

Article Title: Gentiana lutea Root Extract Attenuates Radiation-Induced Damage in Human PBMCs in vitro

doi: 10.1177/15593258261435484

Figure Lengend Snippet: Relative fold change of genes expression involved in DNA repair processes: Ogg1, Parp1 , and Xrcc1 in PBMCs pretreated with 0.25, 0.5, 1 and 2 mg/mL of Gentiana lutea root extract and exposed to 0.5 and 2 Gy irradiation dose

Article Snippet: Expression levels of the target genes were measured by quantitative Real-time PCR on Applied Biosystems Real-Time 7500 system (Applied Biosystems, Inc., Foster City, CA) using TaqMan® gene expression assays: Parp1 (Hs00242302_m1), Ogg1 (Hs00213454_m1), Xrcc1 (Hs00959834_m1).

Techniques: Expressing, Irradiation