Journal: bioRxiv

Article Title: Interleukin-3 as a Potential Bone Anabolic Agent in treating Postmenopausal Osteoporosis

doi: 10.1101/2025.07.12.664485

Figure Lengend Snippet: IL-3 alters OVX-induced changes in osteoclasts- and osteoblasts-specific gene expression. Marrow free bones from IL-3 treated animals from both Prophylactic and Therapeutic treatment strategy, as indicated, were subjected to mRNA expression profile and ( A, B ) Osteoclast-specific markers: integrinβ3, Dc-stamp, and Nfatc1 , Ctsk, Tnfrsf11a(RANK), Mmp9, Trap, and Ctr. (B, D) Osteoblast-specific markers: Runx2, Osx, Opn, Alp, Bmp2, Bmp4, Ocn, Bmp6, and Col1 were assessed by qPCR. Serum ( E, G ) CTX and ( F, H ) OCN were also measured by ELISA in both the strategies. Data is presented as mean ± SEM with n=3 mice/group for qPCR, and n=8 mice/group for ELISA.

Article Snippet: The serum level of CTX-I (CUSABIO; CSB-E12782m) and OCN (CUSABIO ; CSB-E06917m) was assessed by ELISA according to manufacturers’ instructions.

Techniques: Gene Expression, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Food Science & Nutrition

Article Title: Luteolin Inhibits Dexamethasone‐Induced Osteoporosis by Autophagy Activation Through miR‐125b‐5p/SIRT3/AMPK/mTOR Axis, an In Vitro and In Vivo Study

doi: 10.1002/fsn3.70071

Figure Lengend Snippet: Effects of DEX and LUT on BMSC osteogenesis, miR‐125b‐5p expression, and SIRT3/AMPK/mTOR axis. The administration of LUT restored the levels of RUNX2 (a), OSX (b), OPN (c), and OCN (d). Moreover, altered levels of miR‐125b‐5p expression (e), as well as gene expression and protein levels of SIRT3 (f, i), AMPK (g, j), and mTOR (h, k) were obtained after DEX and LUT treatment. p ‐value < 0.05 was considered significant.

Article Snippet: ELISA kits were purchased for the measurement of SIRT3 (Catalogue number: abx156091, abbexa, UK), AMPK (Catalogue number: NBP3‐42385, Novus Biologicals, USA), mTOR (Catalogue number: RTFI01278, AssayGenie, Ireland), Runx2 (Catalogue number: abx255979, abbexa, UK), OSX (Catalogue number: MBS020109, MyBioSource, USA), OPN (Catalogue number: KA0312, Novus Biologicals, USA), OCN (Catalogue number: NBP2‐68153, Novus Biologicals, USA), LC3 (Catalogue number: RTEB1503, AssayGenie, Ireland), ATG5 (Catalogue number: RTEB1055, AssayGenie, Ireland), BECN1 (Catalogue number: NBP2‐69960, Novus Biologicals, USA), and P62 (Catalogue number: NBP2‐61300, Novus Biologicals, USA).

Techniques: Expressing, Gene Expression

Journal: Food Science & Nutrition

Article Title: Luteolin Inhibits Dexamethasone‐Induced Osteoporosis by Autophagy Activation Through miR‐125b‐5p/SIRT3/AMPK/mTOR Axis, an In Vitro and In Vivo Study

doi: 10.1002/fsn3.70071

Figure Lengend Snippet: Effects of DEX and LUT on osteogenesis markers. The administration of LUT restored the gene expression and protein levels of RUNX2 (a, e), OSX (b, f), OPN (c, g), and OCN (d, h). Similar lowercase letters on bars represent no significant difference between groups. p ‐value < 0.05 was considered significant.

Article Snippet: ELISA kits were purchased for the measurement of SIRT3 (Catalogue number: abx156091, abbexa, UK), AMPK (Catalogue number: NBP3‐42385, Novus Biologicals, USA), mTOR (Catalogue number: RTFI01278, AssayGenie, Ireland), Runx2 (Catalogue number: abx255979, abbexa, UK), OSX (Catalogue number: MBS020109, MyBioSource, USA), OPN (Catalogue number: KA0312, Novus Biologicals, USA), OCN (Catalogue number: NBP2‐68153, Novus Biologicals, USA), LC3 (Catalogue number: RTEB1503, AssayGenie, Ireland), ATG5 (Catalogue number: RTEB1055, AssayGenie, Ireland), BECN1 (Catalogue number: NBP2‐69960, Novus Biologicals, USA), and P62 (Catalogue number: NBP2‐61300, Novus Biologicals, USA).

Techniques: Gene Expression