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Differences in colony morphology between Mtb CDC1551, pca::Tn , pca::Tn VC , and pca::Tn comp strains on <t>7H11</t> <t>OADC</t> plates a) containing glycerol as a carbon source b) containing 20 mM pyruvate as a carbon source c) containing 50 mM pyruvate as a carbon source d) containing 70 mM pyruvate as a carbon source. Differences in colony morphology between Mtb CDC1551, pca::Tn , pca::Tn VC , and pca::Tn comp strains on 7H11 OADC CR-CBB agar e) containing glycerol as a carbon source f) containing 20 mM pyruvate as a carbon source g) containing 50 mM pyruvate as a carbon source h) containing 70 mM pyruvate as a carbon source. Images are representative of three independent experiments.
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Differences in colony morphology between Mtb CDC1551, pca::Tn , pca::Tn VC , and pca::Tn comp strains on 7H11 OADC plates a) containing glycerol as a carbon source b) containing 20 mM pyruvate as a carbon source c) containing 50 mM pyruvate as a carbon source d) containing 70 mM pyruvate as a carbon source. Differences in colony morphology between Mtb CDC1551, pca::Tn , pca::Tn VC , and pca::Tn comp strains on 7H11 OADC CR-CBB agar e) containing glycerol as a carbon source f) containing 20 mM pyruvate as a carbon source g) containing 50 mM pyruvate as a carbon source h) containing 70 mM pyruvate as a carbon source. Images are representative of three independent experiments.

Journal: bioRxiv

Article Title: Gluconeogenesis plays a critical role in mycobacterial biofilm formation

doi: 10.1101/2025.03.01.640511

Figure Lengend Snippet: Differences in colony morphology between Mtb CDC1551, pca::Tn , pca::Tn VC , and pca::Tn comp strains on 7H11 OADC plates a) containing glycerol as a carbon source b) containing 20 mM pyruvate as a carbon source c) containing 50 mM pyruvate as a carbon source d) containing 70 mM pyruvate as a carbon source. Differences in colony morphology between Mtb CDC1551, pca::Tn , pca::Tn VC , and pca::Tn comp strains on 7H11 OADC CR-CBB agar e) containing glycerol as a carbon source f) containing 20 mM pyruvate as a carbon source g) containing 50 mM pyruvate as a carbon source h) containing 70 mM pyruvate as a carbon source. Images are representative of three independent experiments.

Article Snippet: For spot assay, 2 μl of each culture logarithmic cultures with similar OD 600 were spotted onto solid Middlebrook 7H11 OADC and 7H11 OADC CRCBB agar containing either glycerol, glucose or pyruvate as a carbon source.

Techniques: