Journal: Redox Biology

Article Title: Oxidative modulation of Piezo1 channels

doi: 10.1016/j.redox.2025.103797

Figure Lengend Snippet: Activation of Piezo1 channels lead to endogenous generation of ROS in Piezo1HEK293T cells : A : example traces of HyPer7 fluorescence reporting generation of endogenous H 2 O 2 in a fraction of cells after application of Yoda1. Notice comparable HyPer7 response induced by application of 1 mM H 2 O 2 . Pie diagram shows that 18 % (118 out from 644) of all cells responded after activation of Piezo1 by Yoda1. B : corresponding bar graphs showing changes in the HyPer7 fluorescence after application of Yoda1 and H 2 O 2 (control: 0.21 ± 0.03 %, n = 210; non-responder Yoda1: 0.3 ± 0.1 %, n = 511; responder Yoda1: 6.52 ± 0.5 %, P < 0.0001, n = 118; H2O2 1 mM: 6.16 ± 0.6 %, n = 122; P < 0.0001, MW test, n number of cells). C , upper: example traces showing no changes in HyPer7 fluorescence in Ca2+-free solution in Piezo1HEK293T cells and similar lack of responses in non-transfected HEK293T and corresponding pie diagrams.

Article Snippet: Transfection was performed using the plasmid DNA encoding human Piezo1 and intracellular H 2 O 2 sensor Hyper7 [ ] ((pCS2+HyPer7-NES was a gift from Vsevolod Belousov (Addgene plasmid # 136467; http://n2t.net/addgene:136467 ; RRID:Addgene_136467)).

Techniques: Activation Assay, Fluorescence, Control, Transfection

Journal: International Journal of Molecular Sciences

Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells

doi: 10.3390/ijms26051939

Figure Lengend Snippet: MTLN silencing decreases endogenous H 2 O 2 levels and reduces sensitivity to exogenous H 2 O 2 toxicity. ( A ) The cytosol- and mitochondria-localized roGFP2-Orp1 H 2 O 2 sensors are less oxidized in siMTLN compared to siCont cultures. Bars show mean ± SD ( n = 3) ** p < 0.01, *** p < 0.001 by unpaired t -test. ( B ) Distinct dose–response effects of H 2 O 2 pulse on cell proliferation in siMTLN compared to siCont cultures. Cultures were exposed to the indicated amount of H 2 O 2 for 3 h and then harvested and counted 24 h later. Bars show mean ± SD ( n = 4) * p < 0.05, **** p < 0.0001 by 2-way ANOVA with multiple comparisons.

Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H 2 O 2 sensors were subcloned from plasmids donated to Addgene from Tobias Dick [ ] (Addgene plasmids #64991 and #64992, Watertown, MA, USA) into a VVC adenovirus shuttle vector (VVC G0688; pacAd5CMVmcspA) using standard cloning techniques, and vector particles (Adcyto-roGFP2-Orp1 and Admito-roGFP2-Orp1) were generated by the VVC.

Techniques: