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antibodies against nup153  (Fortis Life Sciences)
93
Fortis Life Sciences antibodies against nup153
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
Antibodies Against Nup153, supplied by Fortis Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/NUP153+Antibody/pmc13183583-353-27-31
Average 93 stars, based on 1 article reviews
antibodies against nup153 - by Bioz Stars, 2026-10
93/100 stars
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anti nup153 monoclonal antibody  (ABclonal Biotechnology)
94
ABclonal Biotechnology anti nup153 monoclonal antibody
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
Anti Nup153 Monoclonal Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/NUP153+Rabbit+mAb/pmc13325906-270-82-85
Average 94 stars, based on 1 article reviews
anti nup153 monoclonal antibody - by Bioz Stars, 2026-10
94/100 stars
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anti nup153  (Proteintech)
93
Proteintech anti nup153
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
Anti Nup153, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/NUP153+Antibody/pm41677375-267-56-57
Average 93 stars, based on 1 article reviews
anti nup153 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
nup153  (Proteintech)
93
Proteintech nup153
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
Nup153, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/NUP153+Antibody/pm41575851-660-12-24
Average 93 stars, based on 1 article reviews
nup153 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
nup153  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology nup153
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
Nup153, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/Nup153+Antibody/pm41575851-660-12-19
Average 93 stars, based on 1 article reviews
nup153 - by Bioz Stars, 2026-10
93/100 stars
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pegfp c3 nup153  (Addgene inc)
93
Addgene inc pegfp c3 nup153
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
Pegfp C3 Nup153, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/pEGFP(C3)-Nup153+(Plasmid+%2364268)/bio_rxiv__64898__2025__12__12__693814-295-13-14
Average 93 stars, based on 1 article reviews
pegfp c3 nup153 - by Bioz Stars, 2026-10
93/100 stars
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ab170940 anti nup153 antibody  (Proteintech)
93
Proteintech ab170940 anti nup153 antibody
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
Ab170940 Anti Nup153 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/NUP153+Antibody/pmc12480842__41467_2025_63700_MOESM4_ESM-63-49-53
Average 93 stars, based on 1 article reviews
ab170940 anti nup153 antibody - by Bioz Stars, 2026-10
93/100 stars
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1 ap anti flag antibody  (Proteintech)
93
Proteintech 1 ap anti flag antibody
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
1 Ap Anti Flag Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nup153/NUP153+Antibody/pmc12480842__41467_2025_63700_MOESM4_ESM-63-57-69
Average 93 stars, based on 1 article reviews
1 ap anti flag antibody - by Bioz Stars, 2026-10
93/100 stars
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nup153 peptide  (GenScript corporation)
90
GenScript corporation nup153 peptide
( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating <t>Nup153.</t> ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.
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( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating Nup153. ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.

Journal: Nature Physics

Article Title: The local mechanostructural properties of protein cargoes regulate nucleocytoplasmic transport

doi: 10.1038/s41567-026-03242-2

Figure Lengend Snippet: ( A-C ) Nuclear import kinetics of the mCherry protein when imported from the N- or C-terminus upon downregulating Nup153. ( A ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( B ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” mCherry in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( C ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 2.21 ± 0.07; N-term ( siNup153 ) K e = 1.00 ± 0.04; C-term ( siNT ) K e = 1.14 ± 0.03; C-term ( siNup153 ) K e = 0.83 ± 0.02. Import rate: N-term ( siNT ) k I = 3.95 ± 0.14 ks −1 ; N-term ( siNup153 ) k I = 1.59 ± 0.08 ks −1 ; C-term ( siNT ) k I = 0.83 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 1.13 ± 0.04 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = P = 4.00x10 −4 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 9.9x10 −12 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 3.0x10 −6 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 6.7x10 −16 . n = 103 ( siNT N-term); n = 100 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( D-F ) Nuclear import kinetics of the Ig27 when imported from the N- or C- terminus upon downregulating Nup153. ( D ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( E ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Ig27 in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( F ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 1.43 ± 0.04; C-term ( siNT ) K e = 1.15 ± 0.03; N-term ( siNUP153 ) K e = 0.64 ± 0.02; C-term ( siNup153 ) K e = 0.64 ± 0.02. Import rate: N-term ( siNT ) k I = 1.63 ± 0.06 ks −1 ; C-term ( siNT ) k I = 0.97 ± 0.03 ks −1 ; N-term ( siNUP153 ) k I = 0.60 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.44 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 5.2x10 −9 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.71; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNT ) vs. C-term ( siNup153 ) P = 0. Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.3x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 5.2x10 −8 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 0; C-term ( siNup153 ) vs. C-term ( siNT ) P = 0. n = 103 ( siNT N-term); n = 101 ( siNT C-term); n = 85 ( siNUP153 N-term); n = 91 ( siNUP153 C-term). ( G-I ) Nuclear import kinetics of the Spy0128 E117A when imported from the N- or C-terminus upon knocking down Nup153. ( G ) Representative confocal images of U2OS cells after 20 min of the recovery phase, scale bar 10 µm. ( H ) Average time-courses of the nucleus-to-cytoplasm localisation of the “Import forward” and “Import reverse” Spy0128 E117A in the control group ( siNT ) and knocking down Nup153 ( siNup153 ). ( I ) Relative nuclear accumulation (left) and import rate calculated from fits to the recovery time courses. Bars indicate mean. Accumulation: N-term ( siNT ) K e = 0.66 ± 0.22; C-term ( siNT ) K e = 0.54 ± 0.02; N-term ( siNUP153 ) K e = 0.40 ± 0.03; C-term ( siNup153 ) K e = 0.41 ± 0.06. Import rate: N-term ( siNT ) k I = 0.63 ± 0.03 ks −1 ; C-term ( siNT ) k I = 0.29 ± 0.02 ks −1 ; N-term ( siNUP153 ) k I = 0.34 ± 0.02 ks −1 ; C-term ( siNup153 ) k I = 0.16 ± 0.02 ks −1 . Significance levels for two-tailed Mann-Whitney non-parametric test. Accumulation: N-term ( siNT ) vs. C-term ( siNT ) P = 1.0x10 −3 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 0.40; N-term ( siNT ) vs. N-term ( siNup153 ) P = 6.9x10 −10 ; C-term ( siNT ) vs. C-term ( siNup153 ) P = 4.8x10 −3 . Import rate: N-term ( siNT ) vs. C-term ( siNT ) P = 1.2x10 −13 ; N-term ( siNup153 ) vs. C-term ( siNup153 ) P = 4.9x10 −5 ; N-term ( siNT ) vs. N-term ( siNup153 ) P = 3.2x10 −11 ; C-term ( siNup153 ) vs. C-term ( siNT ) P = 1.0x10 −3 . n = 81 ( siNT N-term); n = 37 ( siNT C-term); n = 37 ( siNUP153 N-term); n = 10 ( siNUP153 C-term). ( J ) Percentage of directionality in nuclear accumulation (calculated as the ratio between the N-term and C-term accumulation) for the siNT control group and siNup153 . Error bars indicate SEM. ( K ) Schematics of the proposed role of NUP153 in sensing mechanically labile termini to accelerate nuclear transport.

Article Snippet: Membranes were incubated in a blocking buffer (5% skimmed milk powder in TBS containing 0.2% Tween-20) for 1 h at room temperature and then incubated overnight with antibodies against Nup153 (A301-788A-T, Bethyl Laboratories), importin α3 (MAB8204, R&D Systems) and glyceraldehyde-3-phosphate dehydrogenase (ab8245, Abcam).

Techniques: Control, Two Tailed Test, MANN-WHITNEY