Journal: NPJ Precision Oncology

Article Title: In-depth inference of transcriptional regulatory networks reveals NPM1 as a therapeutic ribosomal regulator in MYC -amplified medulloblastoma

doi: 10.1038/s41698-024-00792-7

Figure Lengend Snippet: a IC50 curves of D458 and D341 cells treated with the NPM1 inhibitor NSC348884 for 8 h. b , c Normalised growth curves of D458, D341 ( b ), and patient-derived MYC -amplified G3 tumour cells ( c ) after treatment with 5 μM and 1 μM NSC348884, respectively; DMSO treated as vehicle, n = 5 biological repeats per group. d Representative immunoblot images of c-Myc, Ser62-phosphorylated c-Myc, and NPM1 in D458 and D341 cells treated with NSC348884 for 8 h. e Immunoblot images of SUnSET in D458 and D341 cells treated with 6 μM NSC348884 for 8 h. Two-way ANOVA for b, c group comparisons; a – c are shown as mean ± s.d.

Article Snippet: For genetic deficiency of NPM1 , cells at a density of 4000 cells per well in 100 μL medium were seeded in 96-well plates, whereas for NSC348884 (MCE) treatment, 2 × 10 4 cells were plated in each well.

Techniques: Derivative Assay, Amplification, Western Blot

Journal: NPJ Precision Oncology

Article Title: In-depth inference of transcriptional regulatory networks reveals NPM1 as a therapeutic ribosomal regulator in MYC -amplified medulloblastoma

doi: 10.1038/s41698-024-00792-7

Figure Lengend Snippet: NPM1 is highly expressed in patients with MYC- amplified MB, corresponding to the upregulated ribosome signature and uncontrolled tumour cell proliferation. Targeting NPM1 with the small-molecule inhibitor NSC348884 may trigger tumour cell apoptosis and hinder MB progression.

Article Snippet: For genetic deficiency of NPM1 , cells at a density of 4000 cells per well in 100 μL medium were seeded in 96-well plates, whereas for NSC348884 (MCE) treatment, 2 × 10 4 cells were plated in each well.

Techniques: Amplification