Journal: Journal of translational medicine

Article Title: RBX1 mitigates ferroptosis by inhibiting NCOA4-mediated ferritinophagy and contributes to the attenuation of intervertebral disc degeneration.

doi: 10.1186/s12967-025-06412-7

Figure Lengend Snippet: Fig. 5 RBX1-mediated ubiquitination modulates NCOA4 expression. A The relative mRNA levels of NCOA4 in acidic and control groups. B, C prediction of E3 ligases targeting NCOA4 as substrate using the online tool UbiBrowser. D, E Stable RBX1-deficient and MDM2-deficient NPSCs were subjected to Western blot analysis to validate the efficiency. F, G Western blot analysis showing the protein expression level of NCOA4 after NPSCs treated with RBX1-siRNA and Control-siRNA. The relative band densities were quantified. H, I Western blot analysis showing the protein expression level of NCOA4 after NPSCs treated with MDM2-siRNA and Control-siRNA. The relative band densities were quantified. J, K Western blot analysis showing the protein expression level of NCOA4 after NPSCs treated with RBX1 overexpression under acidic condition. The relative band densities were quantified. L Co-IP analysis of the binding between NCOA4 and RBX1. M NPSCs were treated with acidic condition for 24 h and the Co-IP analysis of the binding between NCOA4 and RBX1 was shown. N, O Western blot analysis showing the protein expression level of NCOA4 after NPSCs treated with MG132, the proteasome inhibitor. The relative band densities were quantified. A P-value less than 0.05 was deemed to be statistically significant. (*P < 0.05; **P < 0.01; ns, not significant)

Article Snippet: We treated human NPSCs with MG132 (MCE, HY-13259) to suppress proteasome-mediated protein degradation and investigate whether RBX1 destabilizes NCOA4 protein.

Techniques: Ubiquitin Proteomics, Expressing, Control, Western Blot, Over Expression, Co-Immunoprecipitation Assay, Binding Assay

Journal: International journal of molecular sciences

Article Title: Role of Luteolin as Potential New Therapeutic Option for Patients with Glioblastoma through Regulation of Sphingolipid Rheostat.

doi: 10.3390/ijms25010130

Figure Lengend Snippet: Figure 4. Effect of increasing doses of luteolin on astrocytes (A), NPC (B) and NPSC (C). No significant decreases in cell viability were observed, not even at the highest dose tested. Data are the mean ± standard deviation of at least three experiments, run in triplicate.

Article Snippet: Astrocytes were purchased by ABM (Cat. N. T0280), while NPC and NPSC were purchased by CelProgen (Cat. N.: 36057-01 and 36057-02).

Techniques: Standard Deviation

Journal: Nucleic Acids Research

Article Title: RNA/DNA-binding protein TDP43 regulates DNA mismatch repair genes with implications for genome stability

doi: 10.1093/nar/gkaf920

Figure Lengend Snippet: Differential expressions of MMR genes in iPSC-derived neuronal cells and their regulation by TDP43. ( A ) WB images and histogram illustrate a quantitative comparison of MMR expression in control and siTDP43-treated human iPSC-derived neural lineage progenitor stem cells (NPSCs). Quantitation of protein levels normalized to that of GAPDH. ( B ) Schematic of iPSC, NPSC, and terminally differentiated motor neurons (iMN) utilized in this study. ( C ) IF images revealing the expression of TDP43; and MLH1, MSH2, MSH3, MSH6, and PMS2, and nuclear DNA (DAPI) after siTDP43-mediated KD of TDP43 in iPSC-derived iMNs. ( D ) WB images show the expression of select MMR factors across the following states of cellular differentiation: iPSC, NPSC, and iMN. Error bars indicate mean ± SEM from three independent experiments. Significance values (P-values) are as follows P > 0.5 (ns), P <0.5 (*), P <0.001 (***) and P <0.0001 (****).

Article Snippet: Terminally differentiated neurons were derived from neural progenitor stem cells (NPSCs) (Jackson Lab) WT (JIPSC1000), TDP43-Q331K (JIPSC1066), TDP43-A315T, and TDP43-M337V, according to established methods [ ].

Techniques: Derivative Assay, Comparison, Expressing, Control, Quantitation Assay, Cell Differentiation