Journal: Scientific Reports

Article Title: Nox4 and circulating free MPO synergistically promote aortic aneurysm formation

doi: 10.1038/s41598-026-40599-z

Figure Lengend Snippet: Aortic Nox4 expression was induced by AngII and required for MPO uptake ex vivo. ( A – C ) AngII or saline (control) was infused for 3 weeks via osmotic minipump in LDLR KO mice. (A) Aortic Nox4 mRNA expression. ( B ) Aortic H 2 O 2 levels (normalized by tissue weight). ( C ) Representative immunostaining images of Nox4 (white scale bar = 50 µm, red scale bar = 20 µm). ( D ) CaCl 2 or saline (sham) was applied to the infrarenal aorta of C57Bl/6 mice. Representative immunofluorescence images of α-smooth muscle actin and Nox4 (white scale bar = 100 µm). ( E ) Aortic rings harvested from WT, Nox1 KO and Nox4 KO mice were pre-incubated with saline ( −) or AngII ( +) for 12 h, and then treated with or without MPO for 2 h. After washing, MPO activity was then quantified and compared between the different groups. Data are represented as mean ± SEM. ns non-significant, ***p < 0.001.

Article Snippet: MPO KO, Nox4 KO, Nox1 KO, low density lipoprotein receptor (LDLR) KO, and C57Bl/6 mice were obtained from Jackson Laboratories.

Techniques: Expressing, Ex Vivo, Saline, Control, Immunostaining, Immunofluorescence, Incubation, Activity Assay