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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung <t>fibroblasts</t> <t>(HLFs).</t> (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.
Human Lung Fibroblasts Hlfs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung <t>fibroblasts</t> <t>(HLFs).</t> (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.
Human Lung Fibroblasts Cell Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human lung fibroblast cell line hlf  (ATCC)
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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung <t>fibroblasts</t> <t>(HLFs).</t> (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.
Human Lung Fibroblast Cell Line Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+hlf+cells/Primary+Lung+Fibroblast%2C+Normal%2C+Human/10__1080_slash_00958972__2023__2209270-303-40-49
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normal human lung cell lines hlf-a  (Procell Inc)
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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung <t>fibroblasts</t> <t>(HLFs).</t> (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.
Normal Human Lung Cell Lines Hlf A, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung <t>fibroblasts</t> <t>(HLFs).</t> (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.
Human Lung Fibroblast Hlf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+hlf+cells/Primary+Lung+Fibroblast%2C+Normal%2C+Human/pm35512398-59-15-28
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normal human fibroblast cell line hlf  (ATCC)
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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung <t>fibroblasts</t> <t>(HLFs).</t> (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.
Normal Human Fibroblast Cell Line Hlf, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary normal embryo human lung fibroblasts hlf-104 cells  (Biolot)
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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung <t>fibroblasts</t> <t>(HLFs).</t> (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.
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Figure 6. Shrinkage of the hydrogels with 3D cultured human lung fibroblasts (HLFs). (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.

Journal: ACS applied bio materials

Article Title: Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers.

doi: 10.1021/acsabm.3c00126

Figure Lengend Snippet: Figure 6. Shrinkage of the hydrogels with 3D cultured human lung fibroblasts (HLFs). (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). *p ≤0.05 and **p ≤0.01.

Article Snippet: Human bone marrow-derived mesenchymal stromal cells (hBMMSCs) and human lung fibroblasts (HLFs) were purchased from ATCC (PCS-500-012) as well as the culture media containing 125 pg/mL recombinant human fibroblast growth factor (rh FGF), 15 ng/ mL recombinant human insulin-like growth factor-1, 7% fetal bovine serum (FBS), and 2.4 mM L-alanyl-L-glutamine.

Techniques: Cell Culture, Staining