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nmr buffer solution was 25 mm tris-hcl (ph 7.4), 50 mm nacl, 2 mm edta in 90% h 2 o/10% d 2 o  (Daedalus Innovations LLC)
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10× solution of nmr buffer  (Bio-Rad)
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nmr buffer solution supplemented with 2.5 mm nan3  (Fisher Scientific)
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