Journal: eBioMedicine

Article Title: Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates

doi: 10.1016/j.ebiom.2025.105566

Figure Lengend Snippet: Intratumoural delivery of CD44-IR700 enhanced efficacy of photoimmunotherapy in vivo . (a) Schematic of experimental schedule. Mice bearing subcutaneous Lewis lung carcinoma tumours were treated with either no CD44-IR700 (Ctrl), CD44-IR700 via IV injection (CD44-IR700_IV), or CD44-IR700 via IT injection (CD44-IR700_IT), followed by NIR light irradiation on subsequent days. (b) The ratio of tumour-infiltrating CD8-positive T cells in live cells, as determined by flow cytometry on day 7 post-treatment, from the CD44-IR700_IV and CD44-IR700_IT groups (n = 6 per group). (c) Immunohistochemical staining of tumours 7 days after photoimmunotherapy. CD8-positive T cells were stained. The scale bar represents 50 μm. The numbers of CD8-positive T cells per view field were counted (n = 5 mice for each group). (d) Cytokine expression in tumours, as determined by quantitative PCR analysis on day 3 post-treatment with photoimmunotherapy, from the CD44-IR700_IV and CD44-IR700_IT groups (n = 5 per group). (e) Tumour volume monitoring. Tumour size was measured starting from the day of CD44-IR700 administration (day 0), and the change in size was expressed as a ratio relative to the initial volume on day 0 (n = 12 per group). (f) Tumour volume ratio on day 7. The graph shows the fold increase in tumour volume on day 7 compared with that on day 0 (n = 12 per group). (g) Representative images displaying tumours from each treatment group on day 7 post-administration. (h) Representative images of tumours excised on day 7 post-treatment from each therapeutic group. Data are presented as mean + standard error of the mean. Statistical difference was assessed using Mann–Whitney U tests, with significance denoted by asterisks (∗ p < 0.05 and ∗∗∗∗ p < 0.0001). CD44-IR700, IR700-conjugated anti-CD44 monoclonal antibody; CD, cluster of differentiation; NIR, near-infrared; Ctrl, control; IV, intravenous; IT, intratumoural; Ifnγ, interferon-γ; and Tnf, tumour necrosis factor-alpha.

Article Snippet: To assess the viability of LLC cells, 1 h after NIR irradiation, the cell counting kit-8 reagent (CK04, Dojindo) was added to each well and incubated at 37 °C for 1 h. The absorbance of the cell medium was measured at 450 nm using a Spectramax i3X microplate reader (Molecular Devices).

Techniques: In Vivo, IV Injection, Injection, Irradiation, Flow Cytometry, Immunohistochemical staining, Staining, Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY, Control

Journal: eBioMedicine

Article Title: Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates

doi: 10.1016/j.ebiom.2025.105566

Figure Lengend Snippet: The interval between intratumoural administration of CD44-IR700 and NIR irradiation, whether 30 min or 24 h, yields the same effect . (a) Schematic of experimental schedule. Lewis Lung carcinoma cell-induced subcutaneous tumours were established in mice. The mice were divided into three groups for the following treatments: CD44-IR700_IV_24h: IV administration of CD44-IR700 and IT of PBS, with NIR light irradiation 24 h after administration; CD44-IR700_IT_24h: IT administration of CD44-IR700 and IV injection of PBS, followed by NIR light irradiation 24 h later; and CD44-IR700_IT_30m: IT administration of CD44-IR700 and IV injection of PBS, with NIR light irradiation conducted 30 min post-administration. (b) Tumour volume measurement. The tumour volume was monitored from the day of CD44-IR700 administration (designated as day 0), and the change in tumour size was presented as a ratio to the volume on the day of administration (n = 14–18 per group). (c) Tumour volume ratio on day 7 relative to day 0 (n = 14–18 per group). Data are expressed as mean + standard error of the mean. Statistical difference was assessed using Kruskal–Wallis test, with asterisks denoting the levels of significance (∗ p < 0.05). ‘ns’ indicates a lack of statistical significance. CD44-IR700, IR700-conjugated anti-CD44 monoclonal antibody; PBS, phosphate-buffered saline; CD, cluster of differentiation; NIR, near-infrared; Ctrl, control; IV, intravenous; and IT, intratumoural.

Article Snippet: To assess the viability of LLC cells, 1 h after NIR irradiation, the cell counting kit-8 reagent (CK04, Dojindo) was added to each well and incubated at 37 °C for 1 h. The absorbance of the cell medium was measured at 450 nm using a Spectramax i3X microplate reader (Molecular Devices).

Techniques: Irradiation, IV Injection, Saline, Control

Journal: International Journal of Nanomedicine

Article Title: Antibacterial Property and Mechanisms of Au@Ag Core-Shell Nanoparticles with Near-Infrared Absorption Against E. faecalis Infection of Dentin

doi: 10.2147/ijn.s468649

Figure Lengend Snippet: Figure 2 The characteristics and photothermal correlational properties of Au@AgNPs. (A and B) Dynamic light scattering and Zeta potential of products in the formation process of Au@AgNPs and different shell thickness Au@AgNPs. (C and D) UV-vis-NIR spectra of Au@AgNPs incubated in deionized water and PBS (E) PT curves of Au@AgNPs-35 with different concentrations. (F) PT curves of Au@AgNPs-35 under irradiation of different laser powers. (G) PT cycle tests of Au@AgNPs-35 under laser irradiation. (H-K) Thermal images of Au@AgNPs-35 with 100 μg/mL, 200 μg/mL, 300 μg/mL, 400 μg/mL respectively (The white arrow pointing to cementum).

Article Snippet: Furthermore, cytoarchitectural changes in E. faecalis (109 CFUs/mL) incubated with Au@AgNPs (300 μg/mL), with or without NIR irradiation, were analyzed by TEM (HT7800, HITACHI, Tokyo, Japan).

Techniques: Zeta Potential Analyzer, Incubation, Irradiation

Journal: International Journal of Nanomedicine

Article Title: Antibacterial Property and Mechanisms of Au@Ag Core-Shell Nanoparticles with Near-Infrared Absorption Against E. faecalis Infection of Dentin

doi: 10.2147/ijn.s468649

Figure Lengend Snippet: Figure 7 Morphology and structure deformation of E. faecalis incubated with Au@AgNPs at the presence or absence of NIR irradiation. (A) SEM images of E. faecalis treated by PBS without NIR. (B) SEM images of E. faecalis treated by PBS with NIR. (C) SEM images of E. faecalis treated by Au@AgNPs-35 without NIR. (D) SEM images of E. faecalis treated by Au@AgNPs-35 with NIR. (E-H) TEM images of E. faecalis incubated with Au@AgNPs-35. (I-L) TEM images of E. faecalis treated by Au@AgNPs-35 with NIR. (M-P) Elemental mapping of E. faecalis incubated with Au@AgNPs-35. (Q-T) Elemental mapping of E. faecalis treated by Au@AgNPs-35 with NIR. (The blue star indicated intracellular voids and the disintegration of intracellular structures; The red arrows indicated the cell membrane defect.).

Article Snippet: Furthermore, cytoarchitectural changes in E. faecalis (109 CFUs/mL) incubated with Au@AgNPs (300 μg/mL), with or without NIR irradiation, were analyzed by TEM (HT7800, HITACHI, Tokyo, Japan).

Techniques: Incubation, Irradiation, Membrane

Journal: International Journal of Nanomedicine

Article Title: Magnetic Microbubbles Combined with ICG-Loaded Liposomes for Synergistic Mild-Photothermal and Ferroptosis-Enhanced Photodynamic Therapy of Melanoma

doi: 10.2147/IJN.S503753

Figure Lengend Snippet: ( A ) The thermographic images of free Water, free ICG, and ICG-Lips in centrifuge tubes after laser irradiation. (808 nm, 2 W/cm 2 , 3 min). ( B ) Temperature elevation of water, free ICG, and ICG-Lips at the same concentrations of ICG (80 μg/mL) with NIR laser irradiation (808 nm, 2.0 W/cm 2 , 10 min). ( C ) Heating-cooling cycle curve of free ICG and ICG-Lips. ( D ) The spectrum of ABDA absorbance treated with pure laser irradiation for different times in the absence of ICG-Lips. ( E ) The spectrum of ABDA absorbance treated with ICG-Lips under room light for different times. ( F )The spectra of ABDA absorbance treated with ICG-Lips upon different times of laser irradiation (808 nm, 2.0 W/cm 2 , 10 min).

Article Snippet: Briefly, ICG-Lips and ICG were suspended in a solution containing ABDA and irradiated with an 808 nm laser at a power of 2 W/cm 2 , and the UV-Vis spectra of ABDA were recorded before and after NIR irradiation using a Tecan Spark multifunctional microplate reader.

Techniques: Irradiation

Journal: European Radiology Experimental

Article Title: Local hyperthermia mediated by gold nanoparticle-integrated silicone-covered stent: feasibility and tissue response in a rat esophageal model

doi: 10.1186/s41747-024-00438-0

Figure Lengend Snippet: a Photograph showing the stented (AuNP-integrated silicone-covered SEMS) esophagus (arrow) during NIR laser irradiation. b Photothermal image showing the stented (AuNP-integrated silicone-covered SEMS) esophagus (cursor) during NIR laser irradiation. AuNP , Gold nanoparticle; NIR , Near-infrared; SEMS , Self-expandable metal stent

Article Snippet: The sheath was then withdrawn from the stented esophagus, leaving the optic fiber in place, and 808 nm NIR laser irradiation (Lumen Photonics, Beijing, China) was performed at a power output of 1.6 W for 2 min (Fig. b,c).

Techniques: Irradiation

Journal: European Radiology Experimental

Article Title: Local hyperthermia mediated by gold nanoparticle-integrated silicone-covered stent: feasibility and tissue response in a rat esophageal model

doi: 10.1186/s41747-024-00438-0

Figure Lengend Snippet: Line diagram showing the mean in vivo steady-state surface temperatures of the stented (AuNP-integrated silicone-covered SEMS) esophagus with NIR laser irradiation. Error bars are standard deviations. AuNP , Gold nanoparticle; NIR , Near-infrared; SEMS , Self-expandable metal stent

Article Snippet: The sheath was then withdrawn from the stented esophagus, leaving the optic fiber in place, and 808 nm NIR laser irradiation (Lumen Photonics, Beijing, China) was performed at a power output of 1.6 W for 2 min (Fig. b,c).

Techniques: In Vivo, Irradiation