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nikon nis elements deconvolution software  (Nikon)


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    Nikon nikon nis elements deconvolution software
    Nikon Nis Elements Deconvolution Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+elements+deconvolution+software/NIS-Elements/pm41242541-291-33-33
    Average 99 stars, based on 39732 article reviews
    nikon nis elements deconvolution software - by Bioz Stars, 2026-09
    99/100 stars

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    Article Title: Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics
    Article Snippet: .. The widefield Z-stack images were deconvolved using Nikon Elements deconvolution software (Richardson Lucy; parameters: 50 iterations, low noise level). .. Colocalization analysis was performed using the Fiji (ImageJ, National Institute of Health) plugin JACoP (Just Another Co-localization Plugin) to quantify Mander’s correlation coefficients ( ).



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    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Journal: bioRxiv

    Article Title: Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics

    doi: 10.1101/2023.06.03.543566

    Figure Lengend Snippet: A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Article Snippet: The widefield Z-stack images were deconvolved using Nikon Elements deconvolution software (Richardson Lucy; parameters: 50 iterations, low noise level).

    Techniques: Microscopy

    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 60 minutes. Cells were visualized by 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and LAMP1 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with LAMP1-positive lysosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Journal: bioRxiv

    Article Title: Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics

    doi: 10.1101/2023.06.03.543566

    Figure Lengend Snippet: A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 60 minutes. Cells were visualized by 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and LAMP1 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with LAMP1-positive lysosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Article Snippet: The widefield Z-stack images were deconvolved using Nikon Elements deconvolution software (Richardson Lucy; parameters: 50 iterations, low noise level).

    Techniques: Microscopy