Journal: Antioxidants

Article Title: Dynamic Regulation of Ferroptosis in a Neonatal Rat Model of Postnatal Hypoxia-Induced Acute Kidney Injury

doi: 10.3390/antiox15050582

Figure Lengend Snippet: Renal injury and associated biomarkers in the renal cortex of a postnatal hypoxia rat model. ( A ) Expression of the hypoxia marker HIF-1α in the renal cortex, quantified by average optical density (AOD), with HIF-1α-positive staining shown as brown coloration in IHC sections. Representative images were obtained at 200× magnification; scale bar = 50 μm. ( B ) Periodic acid–Schiff (PAS) staining of the renal cortex for the assessment of tubular injury scores, followed by quantitative analysis. Images were acquired at 200× magnification; scale bar = 50 μm. ( C ) Expression of the renal injury marker NGAL in the renal cortex, quantified by AOD, with NGAL-positive staining shown as brown coloration in IHC sections. Images were obtained at 200× magnification; scale bar = 50 μm. ( D ) Glomerular expression of nephrin in each group was quantified as the proportion of nephrin-positive area within the glomerulus, with nephrin staining shown as brown coloration in IHC sections. Images were obtained at 400× magnification; scale bar = 50 μm. For all panels, n = 6 per group. Data distribution was assessed using the Shapiro–Wilk normality test to determine whether parametric or non-parametric statistical analyses were applied. Parameters were analyzed using one-way ANOVA followed by the Sidak post hoc test, and are presented as mean ± SEM ( C , D ). Parameters were analyzed using the Kruskal–Wallis H test followed by Dunn’s post hoc test correction, and are presented as median (IQR) ( A , B ). * p < 0.05 versus normoxia groups (N7 and N14); # p < 0.05 versus H14. In the statistical figures, circles represent the N7 group, squares represent the H7 group, triangles represent the N14 group, inverted triangles represent the H14 group, and diamonds represent the H7N7 group. H7: neonatal rats exposed to hypoxia for 7 days after birth; H14: neonatal rats exposed to hypoxia for 14 days after birth; H7N7: neonatal rats exposed to hypoxia for 7 days followed by normoxia for 7 days; N7: neonatal rats maintained under normoxia for 7 days; N14: neonatal rats maintained under normoxia for 14 days; HIF-1α: hypoxia-inducible factor-1α; NGAL: neutrophil gelatinase-associated lipocalin.

Article Snippet: Primary antibodies included rabbit anti-mouse Alox-12 (1:200; ABclonal, Woburn, MA, USA; A14703), ferritin heavy chain (1:3000; ABclonal; A1144), ferritin light chain (1:100; ABclonal; A21758), FGF-23 (1:200; Bioss, Woburn, MA, USA; bs-5768R), HIF-1α (1:500; Proteintech, Rosemont, IL, USA; A23150), Klotho (1:200; Proteintech; 28100-1-AP), NGAL (1:1000; Abcam; ab63929) and nephrin (1:200; A23150; ABclonal).

Techniques: Expressing, Marker, Staining

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Assay to detect nephrin autoantibodies.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques:

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Detection of serum anti-nephrin autoantibodies by ELISA. (a) Serum levels of anti-nephrin autoantibodies in patients with minimal change disease (MCD), primary focal segmental glomerulosclerosis (pFSGS), membranous nephropathy (MN), lupus nephritis (LN), diabetic nephropathy (DN), IgA nephropathy (IgAN), and healthy controls. (b) Positive rates of anti-nephrin autoantibodies in the same cohorts.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Serum anti-nephrin autoantibody profiles across disease activity states in patients with MCD. (a) Serum levels of anti-nephrin autoantibodies in patients during active phase, partial remission (PR), and complete remission (CR). (b) Positivity rates of anti-nephrin autoantibodies across disease activity states. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques: Activity Assay

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Correlations between serum anti-nephrin autoantibody levels and key clinical parameters.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques:

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Comparison of clinical characteristics between patients positive and negative for anti-nephrin autoantibodies. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques: Comparison

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Anti-nephrin autoantibody IgG subclasses in patients with MCD. (a) Proportion (%) of IgG subclasses among anti-nephrin autoantibodies. (b) Correlation of anti‑nephrin-IgG4 with total anti‑nephrin-IgG. (c) Ratios of anti-nephrin-IgG subclasses indicative of Th1/Th2 skewing: IgG1/IgG4 and IgG3/IgG4.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques:

Journal: medRxiv

Article Title: Nanoscale Podocyte Morphometrics Predict Disease Progression in IgA Nephropathy

doi: 10.64898/2026.03.30.26349728

Figure Lengend Snippet: (a) OCT frozen kidney biopsies were sectioned, cleared and immunolabelled followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of nephrin visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.

Article Snippet: OCT-frozen biopsy specimens were fixed in PFA, cleared in 4% SDS w/ boric acid and fluorescently immunolabelled for nephrin (R&D Systems, cat. no. AF4269) to visualize the slit diaphragm and podocyte foot processes (FPs) based on the protocol described by Unnersjö-Jess et al (details available in Supplement Methods).

Techniques: Imaging