lentiviral negative control virus (Genechem)
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Lentiviral Negative Control Virus, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+virus/control+negative/pmc13276326-10-0-5
Average 86 stars, based on 1 article reviews
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Expressing:Article Title: SphK2/S1P Promotes Metastasis of Triple-Negative Breast Cancer Through the PAK1/LIMK1/Cofilin1 Signaling Pathway Article Snippet: .. Lentivirus expressing SphK2 and corresponding negative Article Title: Astrocyte–neuron crosstalk through extracellular vesicle-shuttled miRNA-382-5p promotes traumatic brain injury Article Snippet: Once the primary neurons reached 70% confluence, they were transfected with 100 nM miRNA-382-5p mimic (sense, 5’-GAAGUUGUUCGUGGUGGAUUCG-3’ and antisense, 5’-AAUCCACCACGAACAACUUCUU-3’) or miRNA mimic NC (sense, 5’-UUCUCCGAACGUGUCACGUTT-3’ and antisense, 5’-ACGUGACACGUUCGGAGAATT-3’) (GenePharma Inc., Shanghai, China) using Lipofectamine RNAiMAX (Thermo Fisher, #13778150) for 24 h, according to the manufacturer’s guidelines. .. A GV314 adenoviral vector expressing OPA1 (Ad-OPA1) and a Article Title: Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma Article Snippet: .. Three recombinant adenoviruses expressing target shRNA (sh-LETM1-1: 5′-GCAGCAAATGATCGGGCAGAT-3′, sh-LETM1-2: 5′-CCAGAGATTGTGGCAAAGGAA-3′, sh-LETM1-3: 5′-GCTATGGATCGACACCAAGAT-3′) and Negative Control:Article Title: SphK2/S1P Promotes Metastasis of Triple-Negative Breast Cancer Through the PAK1/LIMK1/Cofilin1 Signaling Pathway Article Snippet: .. Lentivirus expressing SphK2 and corresponding negative Article Title: Increased CD44 Expression in Endothelial Cells Induced by Advanced Glycation End Products Leads to Insufficient Maturation of Angiogenesis. Article Snippet: Following 1× PBS washes, nuclei were counter- stained with DAPI, and images were captured via laser scanning confocal microscopy (Zeiss LSM880, Germany). .. The CD44- interfering adenovirus (shCD44, Cat#111753–1) and its Article Title: Nrf2 Down-Regulation by Camptothecin Favors Inhibiting Invasion, Metastasis and Angiogenesis in Hepatocellular Carcinoma Article Snippet: .. The negative Article Title: Astrocyte–neuron crosstalk through extracellular vesicle-shuttled miRNA-382-5p promotes traumatic brain injury Article Snippet: Once the primary neurons reached 70% confluence, they were transfected with 100 nM miRNA-382-5p mimic (sense, 5’-GAAGUUGUUCGUGGUGGAUUCG-3’ and antisense, 5’-AAUCCACCACGAACAACUUCUU-3’) or miRNA mimic NC (sense, 5’-UUCUCCGAACGUGUCACGUTT-3’ and antisense, 5’-ACGUGACACGUUCGGAGAATT-3’) (GenePharma Inc., Shanghai, China) using Lipofectamine RNAiMAX (Thermo Fisher, #13778150) for 24 h, according to the manufacturer’s guidelines. .. A GV314 adenoviral vector expressing OPA1 (Ad-OPA1) and a Article Title: Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway. Article Snippet: .. Catwalk XT platform (Noldus Information Technology, Netherlands), high-speed frozen centrifugal machines (Kejun Instrument Company, USA), Nanodrop UV spectrophotometer (Thermofisher, USA), fluorescence quantitative PCR (PALL, 65421), paraffin wheel rotor (Leica, Germany), Sodium pentobarbital, RNA extract kit (Sangon Biotech, Shanghai, B618133-0050), cDNA synthetic kit (Sangon Biotech, Shanghai, B532445-0020), qPCR reaction Mix (Sangon Biotech, Shanghai, B110031-0001), immunohistochemical kit (Sangon Biotech, Shanghai, E607250-0100), Anti-TGF-β1 antibody (Abcam ab92486), Anti-TGF-β3 antibody (Abcam ab15537), Anti-CREB antibody (Abcam ab31387), Anti-Smad3 antibody (Abcam ab208182), Anti-Smad7 antibody (Abcam ab216428), Anti-Collagen I antibody (Abcam ab270993), Anti-Collagen III antibody (Abcam ab7778), LV-Creb1 (Genechem, 43415–1), LV-Creb-RNAi (Genechem, 70035–1), Article Title: Taurine Ameliorates Iron Overload-Induced Hepatocyte Injury via the Bcl-2/VDAC1-Mediated Mitochondrial Apoptosis Pathway Article Snippet: Taurine (purity ≥99.0%) was purchased from Beijing Solarbio Science & Technology Co. (Beijing, China), Ltd., and iron dextran was purchased from Sigma-Aldrich (St. Louis, Missouri, USA). .. Lentivirus siRNA Bcl-2 and a Article Title: Oestrogen up-regulates DNMT1 and leads to the hypermethylation of RUNX3 in the malignant transformation of ovarian endometriosis. Article Snippet: .. The lentivirus containing RUNX3 siRNA sequence and Article Title: Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma Article Snippet: .. Three recombinant adenoviruses expressing target shRNA (sh-LETM1-1: 5′-GCAGCAAATGATCGGGCAGAT-3′, sh-LETM1-2: 5′-CCAGAGATTGTGGCAAAGGAA-3′, sh-LETM1-3: 5′-GCTATGGATCGACACCAAGAT-3′) and Virus:Article Title: SphK2/S1P Promotes Metastasis of Triple-Negative Breast Cancer Through the PAK1/LIMK1/Cofilin1 Signaling Pathway Article Snippet: .. Lentivirus expressing SphK2 and corresponding negative Article Title: Increased CD44 Expression in Endothelial Cells Induced by Advanced Glycation End Products Leads to Insufficient Maturation of Angiogenesis. Article Snippet: Following 1× PBS washes, nuclei were counter- stained with DAPI, and images were captured via laser scanning confocal microscopy (Zeiss LSM880, Germany). .. The CD44- interfering adenovirus (shCD44, Cat#111753–1) and its Article Title: Nrf2 Down-Regulation by Camptothecin Favors Inhibiting Invasion, Metastasis and Angiogenesis in Hepatocellular Carcinoma Article Snippet: .. The negative Article Title: Astrocyte–neuron crosstalk through extracellular vesicle-shuttled miRNA-382-5p promotes traumatic brain injury Article Snippet: Once the primary neurons reached 70% confluence, they were transfected with 100 nM miRNA-382-5p mimic (sense, 5’-GAAGUUGUUCGUGGUGGAUUCG-3’ and antisense, 5’-AAUCCACCACGAACAACUUCUU-3’) or miRNA mimic NC (sense, 5’-UUCUCCGAACGUGUCACGUTT-3’ and antisense, 5’-ACGUGACACGUUCGGAGAATT-3’) (GenePharma Inc., Shanghai, China) using Lipofectamine RNAiMAX (Thermo Fisher, #13778150) for 24 h, according to the manufacturer’s guidelines. .. A GV314 adenoviral vector expressing OPA1 (Ad-OPA1) and a Article Title: Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway. Article Snippet: .. Catwalk XT platform (Noldus Information Technology, Netherlands), high-speed frozen centrifugal machines (Kejun Instrument Company, USA), Nanodrop UV spectrophotometer (Thermofisher, USA), fluorescence quantitative PCR (PALL, 65421), paraffin wheel rotor (Leica, Germany), Sodium pentobarbital, RNA extract kit (Sangon Biotech, Shanghai, B618133-0050), cDNA synthetic kit (Sangon Biotech, Shanghai, B532445-0020), qPCR reaction Mix (Sangon Biotech, Shanghai, B110031-0001), immunohistochemical kit (Sangon Biotech, Shanghai, E607250-0100), Anti-TGF-β1 antibody (Abcam ab92486), Anti-TGF-β3 antibody (Abcam ab15537), Anti-CREB antibody (Abcam ab31387), Anti-Smad3 antibody (Abcam ab208182), Anti-Smad7 antibody (Abcam ab216428), Anti-Collagen I antibody (Abcam ab270993), Anti-Collagen III antibody (Abcam ab7778), LV-Creb1 (Genechem, 43415–1), LV-Creb-RNAi (Genechem, 70035–1), Article Title: Taurine Ameliorates Iron Overload-Induced Hepatocyte Injury via the Bcl-2/VDAC1-Mediated Mitochondrial Apoptosis Pathway Article Snippet: Taurine (purity ≥99.0%) was purchased from Beijing Solarbio Science & Technology Co. (Beijing, China), Ltd., and iron dextran was purchased from Sigma-Aldrich (St. Louis, Missouri, USA). .. Lentivirus siRNA Bcl-2 and a Article Title: Oestrogen up-regulates DNMT1 and leads to the hypermethylation of RUNX3 in the malignant transformation of ovarian endometriosis. Article Snippet: .. The lentivirus containing RUNX3 siRNA sequence and Article Title: Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma Article Snippet: .. Three recombinant adenoviruses expressing target shRNA (sh-LETM1-1: 5′-GCAGCAAATGATCGGGCAGAT-3′, sh-LETM1-2: 5′-CCAGAGATTGTGGCAAAGGAA-3′, sh-LETM1-3: 5′-GCTATGGATCGACACCAAGAT-3′) and Plasmid Preparation:Article Title: Astrocyte–neuron crosstalk through extracellular vesicle-shuttled miRNA-382-5p promotes traumatic brain injury Article Snippet: Once the primary neurons reached 70% confluence, they were transfected with 100 nM miRNA-382-5p mimic (sense, 5’-GAAGUUGUUCGUGGUGGAUUCG-3’ and antisense, 5’-AAUCCACCACGAACAACUUCUU-3’) or miRNA mimic NC (sense, 5’-UUCUCCGAACGUGUCACGUTT-3’ and antisense, 5’-ACGUGACACGUUCGGAGAATT-3’) (GenePharma Inc., Shanghai, China) using Lipofectamine RNAiMAX (Thermo Fisher, #13778150) for 24 h, according to the manufacturer’s guidelines. .. A GV314 adenoviral vector expressing OPA1 (Ad-OPA1) and a Construct:Article Title: Astrocyte–neuron crosstalk through extracellular vesicle-shuttled miRNA-382-5p promotes traumatic brain injury Article Snippet: Once the primary neurons reached 70% confluence, they were transfected with 100 nM miRNA-382-5p mimic (sense, 5’-GAAGUUGUUCGUGGUGGAUUCG-3’ and antisense, 5’-AAUCCACCACGAACAACUUCUU-3’) or miRNA mimic NC (sense, 5’-UUCUCCGAACGUGUCACGUTT-3’ and antisense, 5’-ACGUGACACGUUCGGAGAATT-3’) (GenePharma Inc., Shanghai, China) using Lipofectamine RNAiMAX (Thermo Fisher, #13778150) for 24 h, according to the manufacturer’s guidelines. .. A GV314 adenoviral vector expressing OPA1 (Ad-OPA1) and a Article Title: Taurine Ameliorates Iron Overload-Induced Hepatocyte Injury via the Bcl-2/VDAC1-Mediated Mitochondrial Apoptosis Pathway Article Snippet: Taurine (purity ≥99.0%) was purchased from Beijing Solarbio Science & Technology Co. (Beijing, China), Ltd., and iron dextran was purchased from Sigma-Aldrich (St. Louis, Missouri, USA). .. Lentivirus siRNA Bcl-2 and a Spectrophotometry:Article Title: Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway. Article Snippet: .. Catwalk XT platform (Noldus Information Technology, Netherlands), high-speed frozen centrifugal machines (Kejun Instrument Company, USA), Nanodrop UV spectrophotometer (Thermofisher, USA), fluorescence quantitative PCR (PALL, 65421), paraffin wheel rotor (Leica, Germany), Sodium pentobarbital, RNA extract kit (Sangon Biotech, Shanghai, B618133-0050), cDNA synthetic kit (Sangon Biotech, Shanghai, B532445-0020), qPCR reaction Mix (Sangon Biotech, Shanghai, B110031-0001), immunohistochemical kit (Sangon Biotech, Shanghai, E607250-0100), Anti-TGF-β1 antibody (Abcam ab92486), Anti-TGF-β3 antibody (Abcam ab15537), Anti-CREB antibody (Abcam ab31387), Anti-Smad3 antibody (Abcam ab208182), Anti-Smad7 antibody (Abcam ab216428), Anti-Collagen I antibody (Abcam ab270993), Anti-Collagen III antibody (Abcam ab7778), LV-Creb1 (Genechem, 43415–1), LV-Creb-RNAi (Genechem, 70035–1), Fluorescence:Article Title: Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway. Article Snippet: .. Catwalk XT platform (Noldus Information Technology, Netherlands), high-speed frozen centrifugal machines (Kejun Instrument Company, USA), Nanodrop UV spectrophotometer (Thermofisher, USA), fluorescence quantitative PCR (PALL, 65421), paraffin wheel rotor (Leica, Germany), Sodium pentobarbital, RNA extract kit (Sangon Biotech, Shanghai, B618133-0050), cDNA synthetic kit (Sangon Biotech, Shanghai, B532445-0020), qPCR reaction Mix (Sangon Biotech, Shanghai, B110031-0001), immunohistochemical kit (Sangon Biotech, Shanghai, E607250-0100), Anti-TGF-β1 antibody (Abcam ab92486), Anti-TGF-β3 antibody (Abcam ab15537), Anti-CREB antibody (Abcam ab31387), Anti-Smad3 antibody (Abcam ab208182), Anti-Smad7 antibody (Abcam ab216428), Anti-Collagen I antibody (Abcam ab270993), Anti-Collagen III antibody (Abcam ab7778), LV-Creb1 (Genechem, 43415–1), LV-Creb-RNAi (Genechem, 70035–1), Real-time Polymerase Chain Reaction:Article Title: Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway. Article Snippet: .. Catwalk XT platform (Noldus Information Technology, Netherlands), high-speed frozen centrifugal machines (Kejun Instrument Company, USA), Nanodrop UV spectrophotometer (Thermofisher, USA), fluorescence quantitative PCR (PALL, 65421), paraffin wheel rotor (Leica, Germany), Sodium pentobarbital, RNA extract kit (Sangon Biotech, Shanghai, B618133-0050), cDNA synthetic kit (Sangon Biotech, Shanghai, B532445-0020), qPCR reaction Mix (Sangon Biotech, Shanghai, B110031-0001), immunohistochemical kit (Sangon Biotech, Shanghai, E607250-0100), Anti-TGF-β1 antibody (Abcam ab92486), Anti-TGF-β3 antibody (Abcam ab15537), Anti-CREB antibody (Abcam ab31387), Anti-Smad3 antibody (Abcam ab208182), Anti-Smad7 antibody (Abcam ab216428), Anti-Collagen I antibody (Abcam ab270993), Anti-Collagen III antibody (Abcam ab7778), LV-Creb1 (Genechem, 43415–1), LV-Creb-RNAi (Genechem, 70035–1), Immunohistochemical staining:Article Title: Up-regulation of CREB-1 regulates tendon adhesion in the injury tendon healing through the CREB-1/TGF-β3 signaling pathway. Article Snippet: .. Catwalk XT platform (Noldus Information Technology, Netherlands), high-speed frozen centrifugal machines (Kejun Instrument Company, USA), Nanodrop UV spectrophotometer (Thermofisher, USA), fluorescence quantitative PCR (PALL, 65421), paraffin wheel rotor (Leica, Germany), Sodium pentobarbital, RNA extract kit (Sangon Biotech, Shanghai, B618133-0050), cDNA synthetic kit (Sangon Biotech, Shanghai, B532445-0020), qPCR reaction Mix (Sangon Biotech, Shanghai, B110031-0001), immunohistochemical kit (Sangon Biotech, Shanghai, E607250-0100), Anti-TGF-β1 antibody (Abcam ab92486), Anti-TGF-β3 antibody (Abcam ab15537), Anti-CREB antibody (Abcam ab31387), Anti-Smad3 antibody (Abcam ab208182), Anti-Smad7 antibody (Abcam ab216428), Anti-Collagen I antibody (Abcam ab270993), Anti-Collagen III antibody (Abcam ab7778), LV-Creb1 (Genechem, 43415–1), LV-Creb-RNAi (Genechem, 70035–1), Sequencing:Article Title: Oestrogen up-regulates DNMT1 and leads to the hypermethylation of RUNX3 in the malignant transformation of ovarian endometriosis. Article Snippet: .. The lentivirus containing RUNX3 siRNA sequence and Transfection:Article Title: Oestrogen up-regulates DNMT1 and leads to the hypermethylation of RUNX3 in the malignant transformation of ovarian endometriosis. Article Snippet: .. The lentivirus containing RUNX3 siRNA sequence and Recombinant:Article Title: Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma Article Snippet: .. Three recombinant adenoviruses expressing target shRNA (sh-LETM1-1: 5′-GCAGCAAATGATCGGGCAGAT-3′, sh-LETM1-2: 5′-CCAGAGATTGTGGCAAAGGAA-3′, sh-LETM1-3: 5′-GCTATGGATCGACACCAAGAT-3′) and shRNA:Article Title: Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma Article Snippet: .. Three recombinant adenoviruses expressing target shRNA (sh-LETM1-1: 5′-GCAGCAAATGATCGGGCAGAT-3′, sh-LETM1-2: 5′-CCAGAGATTGTGGCAAAGGAA-3′, sh-LETM1-3: 5′-GCTATGGATCGACACCAAGAT-3′) and Synthesized:Article Title: Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma Article Snippet: .. Three recombinant adenoviruses expressing target shRNA (sh-LETM1-1: 5′-GCAGCAAATGATCGGGCAGAT-3′, sh-LETM1-2: 5′-CCAGAGATTGTGGCAAAGGAA-3′, sh-LETM1-3: 5′-GCTATGGATCGACACCAAGAT-3′) and |
