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Laser Doppler flowmetry of blood flow in the middle cerebral artery (MCA) territory during peri-MCAO periods. A. A representative Laser Doppler flowmetry: (i) Pre-MCAO: the MCA blood flow baseline before CCAO and MCAO; (ii) CCA (temporal) occlusion during the MCAO period: MCA blood flow was reduced after common carotid artery occlusion (CCAO); (iii) MCAO period: MCA blood flow was further reduced to the >90% of the pre-MCAO level after CCAO and MCAO with the monofilament; and (iv) Reperfusion: MCA blood flow was resumed close to the pre-ischemia level after release of MCAO and CCAO to start reperfusion. B. The MCA blood flow in CC (triangle, n=13), SC (square, n=15), and NT (circle, n=11) groups during peri-MCAO periods. The MCA blood flow declined significantly during the period from the 10 min to 210 min reperfusion among CC, SC or NT groups. Data shown are mean ± SEM, **p<0.01 between 10 vs. 210 min reperfusion among all CC, SC or NT groups; no statistically significant differences between CC or SC vs NT groups, one-way ANOVA followed by Tukey test.

Journal: bioRxiv

Article Title: Targeted Intestinal Cooling Offers Superior Brain Protection in the Mouse Stroke Model

doi: 10.1101/2025.10.10.681764

Figure Lengend Snippet: Laser Doppler flowmetry of blood flow in the middle cerebral artery (MCA) territory during peri-MCAO periods. A. A representative Laser Doppler flowmetry: (i) Pre-MCAO: the MCA blood flow baseline before CCAO and MCAO; (ii) CCA (temporal) occlusion during the MCAO period: MCA blood flow was reduced after common carotid artery occlusion (CCAO); (iii) MCAO period: MCA blood flow was further reduced to the >90% of the pre-MCAO level after CCAO and MCAO with the monofilament; and (iv) Reperfusion: MCA blood flow was resumed close to the pre-ischemia level after release of MCAO and CCAO to start reperfusion. B. The MCA blood flow in CC (triangle, n=13), SC (square, n=15), and NT (circle, n=11) groups during peri-MCAO periods. The MCA blood flow declined significantly during the period from the 10 min to 210 min reperfusion among CC, SC or NT groups. Data shown are mean ± SEM, **p<0.01 between 10 vs. 210 min reperfusion among all CC, SC or NT groups; no statistically significant differences between CC or SC vs NT groups, one-way ANOVA followed by Tukey test.

Article Snippet: Briefly, the middle cerebral artery (MCA) blood flow was monitored through a small incision to attach a needle laser Doppler flowmetry (LDF) probe (MNP100XP, AD Instruments, CO, USA) to the surface of the temporal bone (Supplement Fig. 2A).

Techniques: