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Arctiin alleviated oxidative injury lured by DOX in mice. (A–C) Representative western blot images and statistical results (n = 3). (D–F) D represents 4-HNE and DHE staining of hearts, E and F represent statistical results of of two kinds of staining respectively (n = 3). (G–K) The contents of GSH level, CAT activity, NADPH oxidase activity, SOD activity, and MDA in hearts (n = 6). (L) Representative of mRNA level of oxidative stress related genes (n = 3). Data were presented as mean ±S.D.

Journal: Frontiers in Pharmacology

Article Title: Targeting SIRT1 stability: arctiin ameliorates doxorubicin-induced cardiac injury by inhibiting SMURF2 binding and enhancing protective lipid metabolism

doi: 10.3389/fphar.2026.1797815

Figure Lengend Snippet: Arctiin alleviated oxidative injury lured by DOX in mice. (A–C) Representative western blot images and statistical results (n = 3). (D–F) D represents 4-HNE and DHE staining of hearts, E and F represent statistical results of of two kinds of staining respectively (n = 3). (G–K) The contents of GSH level, CAT activity, NADPH oxidase activity, SOD activity, and MDA in hearts (n = 6). (L) Representative of mRNA level of oxidative stress related genes (n = 3). Data were presented as mean ±S.D.

Article Snippet: NADPH oxidase assay kit, total SOD assay kit, Malondialdehyde (MDA) assay kit, and Caspase-3 activity assay kit were all generated by Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

Techniques: Western Blot, Staining, Activity Assay