Journal: Poultry Science

Article Title: Screening and characterization of Limosilactobacillus reuteri L16 and its probiotic properties in Hy-Line Brown chicks

doi: 10.1016/j.psj.2026.107024

Figure Lengend Snippet: Evaluation of cell surface properties and safety of LAB. (A) Auto-aggregation ability of LAB after 3, 6, and 24 h of static incubation. Statistical significance was assessed relative to strain L2 at 3 and 24 h and relative to strain L5 at 6 h; (B) Cell surface hydrophobicity of LAB assessed using n-hexadecane, ethyl acetate, and xylene, with statistical significance determined relative to strain L2. (C) Adhesion of LAB strains to ileal tissue, with statistical significance determined relative to strain L2. (D) Hemolytic activity of LAB. Data are shown as mean ± SD ( n = 3). Statistical analyses for panels (A–C) were performed using ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. Ns indicates no significant difference; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: For the hydrophobicity assay, 3 mL of bacterial suspension was mixed separately with 1 mL of xylene, ethyl acetate (Chronchem, Chengdu, China), or n-hexadecane (Macklin, Shanghai, China).

Techniques: Incubation, Cell Surface Hydrophobicity, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: High Cell Density Fermentation of Yarrowia lipolytica on n-Hexadecane for the Valorization of Pyrolyzed Plastic Waste

doi: 10.3390/ijms27021107

Figure Lengend Snippet: Average final OD of 23 strains after growing for three days on SC + 20 vol% hydrocarbon (n-octane, n-decane, n-dodecane, and n-hexadecane).

Article Snippet: After 18–20 h, 6 mL were transferred to 141 mL YNB medium (4.2 g·L −1 yeast nitrogen base without amino acids and without ammonium sulfate, 5 g·L −1 (NH 4 ) 2 SO 4 , and 3 g·L −1 Hy-Yest 412 yeast extract (YE) (Tralee, Ireland)) set to a pH of 5.5, and 3 mL hexadecane (>95% purity, Thermo Fisher, Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: High Cell Density Fermentation of Yarrowia lipolytica on n-Hexadecane for the Valorization of Pyrolyzed Plastic Waste

doi: 10.3390/ijms27021107

Figure Lengend Snippet: Y. lipolytica strains Yl1 (W29 Y. lipolytica reference lab strain) and Yl52 OD 600 measurements over time of non-engineered vs. Δ mhy1 on SC + 10 vol% n-hexadecane in shaking flasks.

Article Snippet: After 18–20 h, 6 mL were transferred to 141 mL YNB medium (4.2 g·L −1 yeast nitrogen base without amino acids and without ammonium sulfate, 5 g·L −1 (NH 4 ) 2 SO 4 , and 3 g·L −1 Hy-Yest 412 yeast extract (YE) (Tralee, Ireland)) set to a pH of 5.5, and 3 mL hexadecane (>95% purity, Thermo Fisher, Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: High Cell Density Fermentation of Yarrowia lipolytica on n-Hexadecane for the Valorization of Pyrolyzed Plastic Waste

doi: 10.3390/ijms27021107

Figure Lengend Snippet: Y. lipolytica Yl52 Δ mhy1 grown on n-hexadecane ( A ) settled for a few minutes, ( B ) centrifuged for 5 min at 14,200 rpm, and ( C ) microscopic image of n-hexadecane droplet surrounded by adsorbed cells.

Article Snippet: After 18–20 h, 6 mL were transferred to 141 mL YNB medium (4.2 g·L −1 yeast nitrogen base without amino acids and without ammonium sulfate, 5 g·L −1 (NH 4 ) 2 SO 4 , and 3 g·L −1 Hy-Yest 412 yeast extract (YE) (Tralee, Ireland)) set to a pH of 5.5, and 3 mL hexadecane (>95% purity, Thermo Fisher, Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: High Cell Density Fermentation of Yarrowia lipolytica on n-Hexadecane for the Valorization of Pyrolyzed Plastic Waste

doi: 10.3390/ijms27021107

Figure Lengend Snippet: Cell dry weights (g·L −1 ) of Y. lipolytica Yl52 Δ mhy1 obtained by Standard Filtration Protocol (1), Filtration with n-Hexane Wash (2), and Surfactant Wash with Tween 80 (3) grown on 5 and 10 vol% of hexadecane compared to glucose. Statistical differences were assessed with pairwise t -tests; the results are shown as not different (nd, p > 0.05) or significant (*, p < 0.05).

Article Snippet: After 18–20 h, 6 mL were transferred to 141 mL YNB medium (4.2 g·L −1 yeast nitrogen base without amino acids and without ammonium sulfate, 5 g·L −1 (NH 4 ) 2 SO 4 , and 3 g·L −1 Hy-Yest 412 yeast extract (YE) (Tralee, Ireland)) set to a pH of 5.5, and 3 mL hexadecane (>95% purity, Thermo Fisher, Waltham, MA, USA).

Techniques: Filtration

Journal: International Journal of Molecular Sciences

Article Title: High Cell Density Fermentation of Yarrowia lipolytica on n-Hexadecane for the Valorization of Pyrolyzed Plastic Waste

doi: 10.3390/ijms27021107

Figure Lengend Snippet: Cell dry weight (g·L −1 ) of Y. lipolytica Yl52 Δ mhy1 grown on 2 vol%, 10 vol%, 20 vol% of n-hexadecane, and without a carbon source. Groups that share a letter are not significantly different; groups with different letters differ significantly ( p < 0.05, Bonferroni-corrected α = 0.0167), assessed with pairwise t -tests for each day.

Article Snippet: After 18–20 h, 6 mL were transferred to 141 mL YNB medium (4.2 g·L −1 yeast nitrogen base without amino acids and without ammonium sulfate, 5 g·L −1 (NH 4 ) 2 SO 4 , and 3 g·L −1 Hy-Yest 412 yeast extract (YE) (Tralee, Ireland)) set to a pH of 5.5, and 3 mL hexadecane (>95% purity, Thermo Fisher, Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: High Cell Density Fermentation of Yarrowia lipolytica on n-Hexadecane for the Valorization of Pyrolyzed Plastic Waste

doi: 10.3390/ijms27021107

Figure Lengend Snippet: Cultivation of Y. lipolytica Yl52 Δ mhy1 at pH 4.0 ( A ) and pH 2.5 ( B ) in 4 L bioreactors: biomass concentration g·L −1 (CDW), n-hexadecane (vol%), citrate concentration g·L −1 . Vertical lines indicate pulsed feedings of 150 mL of n-hexadecane.

Article Snippet: After 18–20 h, 6 mL were transferred to 141 mL YNB medium (4.2 g·L −1 yeast nitrogen base without amino acids and without ammonium sulfate, 5 g·L −1 (NH 4 ) 2 SO 4 , and 3 g·L −1 Hy-Yest 412 yeast extract (YE) (Tralee, Ireland)) set to a pH of 5.5, and 3 mL hexadecane (>95% purity, Thermo Fisher, Waltham, MA, USA).

Techniques: Concentration Assay