Journal: Frontiers in Pharmacology

Article Title: Protective effect of hesperetin against myelosuppression and splenic inflammation via modulating TLR4/MYD88/NF-κB signaling: molecular docking and experimental validation

doi: 10.3389/fphar.2026.1826774

Figure Lengend Snippet: (A) List of ten intersecting genes of TLR–MYD88 pathway interactors that are simultaneously targeted by hesperetin. (B) Lollipop KEGG pathways enrichment chart of the top 10 pathways, color coded and sorted by fold enrichment. Chart was constructed using STRING db (FDR: false discovery rate).

Article Snippet: Then, a citrate buffer solution was added to the tissues and heating was done in a microwave for 24 min. Then, a diluted solution of MYD88 rabbit mAB (1:200, A19082, ABclonal) was added.

Techniques: Construct

Journal: Frontiers in Pharmacology

Article Title: Protective effect of hesperetin against myelosuppression and splenic inflammation via modulating TLR4/MYD88/NF-κB signaling: molecular docking and experimental validation

doi: 10.3389/fphar.2026.1826774

Figure Lengend Snippet: Effect of hesperetin on MYD88 immunohistochemical staining in specimens from mice spleens. (A) Saline control group: Images show a normal MYD88 immunostaining pattern. (B) Vincristine group: show marked increase of MYD88%, correlating with lymphoid depletion. (C) Vincristine + hesperetin group: the MYD88 staining was increased versus the vincristine group. (D1,D2) The hesperetin per se group showed immunostaining similar to the saline group. (E) Column charts for the area of immunostaining, data are mean ± SD. * versus the saline group, # versus vincristine group (P < 0.05).

Article Snippet: Then, a citrate buffer solution was added to the tissues and heating was done in a microwave for 24 min. Then, a diluted solution of MYD88 rabbit mAB (1:200, A19082, ABclonal) was added.

Techniques: Immunohistochemical staining, Staining, Saline, Control, Immunostaining

Journal: Frontiers in Pharmacology

Article Title: Protective effect of hesperetin against myelosuppression and splenic inflammation via modulating TLR4/MYD88/NF-κB signaling: molecular docking and experimental validation

doi: 10.3389/fphar.2026.1826774

Figure Lengend Snippet: Effect of hesperetin on the vincristine induced target biomarkers in the spleen. Bar charts demonstrate inflammatory markers detected by ELISA in spleen supernatants of (A) TLR4, (B) NF-κB, (C) MYD88, and (D) TNF-α, and (E) IL-1β. Data are demonstrated as mean ± SD. * versus the saline group, # versus vincristine group (P < 0.05).

Article Snippet: Then, a citrate buffer solution was added to the tissues and heating was done in a microwave for 24 min. Then, a diluted solution of MYD88 rabbit mAB (1:200, A19082, ABclonal) was added.

Techniques: Enzyme-linked Immunosorbent Assay, Saline

Journal: Frontiers in Pharmacology

Article Title: Protective effect of hesperetin against myelosuppression and splenic inflammation via modulating TLR4/MYD88/NF-κB signaling: molecular docking and experimental validation

doi: 10.3389/fphar.2026.1826774

Figure Lengend Snippet: Correlation using Pearson’s correlation analysis between TLR4 and the other inflammatory markers. The correlation between TLR4 and (A) MYD88, (B) NF-κB, (C) TNF-α and (D) IL-1β are shown.

Article Snippet: Then, a citrate buffer solution was added to the tissues and heating was done in a microwave for 24 min. Then, a diluted solution of MYD88 rabbit mAB (1:200, A19082, ABclonal) was added.

Techniques: