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Representative photographs of the 3D tumor-stroma model with GFP transfected HUVEC and tumor spheroids of untransfected H838 tumor cells on day 20. ( A ): Brightfield picture shows a spheroid (S) and an <t>endothelial</t> cell network (EC). The ECs form several tubule-like structures (T) in all directions. ( B ): GFP fluorescence photo of A also shows the GFP-positive endothelial cell (EC) within the tubule-like structures (T). The spheroid (S) is GFP-negative. ( C ): Merged picture of ( A , B ). The GFP-negative spheroid (S) and the GFP-positive endothelial cell (EC) with the tubule-like structures (T) can be observed. These structures extend into all directions. Measuring bar: 100 µm.
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Representative photographs of the 3D tumor-stroma model with GFP transfected HUVEC and tumor spheroids of untransfected H838 tumor cells on day 20. ( A ): Brightfield picture shows a spheroid (S) and an endothelial cell network (EC). The ECs form several tubule-like structures (T) in all directions. ( B ): GFP fluorescence photo of A also shows the GFP-positive endothelial cell (EC) within the tubule-like structures (T). The spheroid (S) is GFP-negative. ( C ): Merged picture of ( A , B ). The GFP-negative spheroid (S) and the GFP-positive endothelial cell (EC) with the tubule-like structures (T) can be observed. These structures extend into all directions. Measuring bar: 100 µm.

Journal: Bioengineering

Article Title: Recapitulating the Angiogenic Switch in a Hydrogel-Based 3D In Vitro Tumor-Stroma Model

doi: 10.3390/bioengineering8110186

Figure Lengend Snippet: Representative photographs of the 3D tumor-stroma model with GFP transfected HUVEC and tumor spheroids of untransfected H838 tumor cells on day 20. ( A ): Brightfield picture shows a spheroid (S) and an endothelial cell network (EC). The ECs form several tubule-like structures (T) in all directions. ( B ): GFP fluorescence photo of A also shows the GFP-positive endothelial cell (EC) within the tubule-like structures (T). The spheroid (S) is GFP-negative. ( C ): Merged picture of ( A , B ). The GFP-negative spheroid (S) and the GFP-positive endothelial cell (EC) with the tubule-like structures (T) can be observed. These structures extend into all directions. Measuring bar: 100 µm.

Article Snippet: Endothelial cells: Human umbilical vein endothelial cells (HUVEC) and GFP transfected HUVEC (Pelobiotech, Martinsried, Germany) were maintained in Endothelial Cell Growth Medium (MVEM, Pelobiotech, Martinsried, Germany) containing all supplements and subcultured at 80% confluency.

Techniques: Transfection, Fluorescence

( A ): The bar graph shows a significant reduction in spheroid size after treatment with both concentrations of cisplatin compared to control. ( B ): Fluorescence photomicrographs of hydrogel cultures containing H838GFP tumor spheroids (dark green), RFP fibroblasts (light green), inflammatory cells and endothelial cells after 10, 14 and 17 days of treatment with 50 μM and 250 μM. In comparison to control cultures, cisplatin does not reduce the number of fibroblasts but negatively affects the fibroblast network as well as fibroblast morphology which changes towards small spindle shaped cells. Measuring bar: 100 µm.

Journal: Bioengineering

Article Title: Recapitulating the Angiogenic Switch in a Hydrogel-Based 3D In Vitro Tumor-Stroma Model

doi: 10.3390/bioengineering8110186

Figure Lengend Snippet: ( A ): The bar graph shows a significant reduction in spheroid size after treatment with both concentrations of cisplatin compared to control. ( B ): Fluorescence photomicrographs of hydrogel cultures containing H838GFP tumor spheroids (dark green), RFP fibroblasts (light green), inflammatory cells and endothelial cells after 10, 14 and 17 days of treatment with 50 μM and 250 μM. In comparison to control cultures, cisplatin does not reduce the number of fibroblasts but negatively affects the fibroblast network as well as fibroblast morphology which changes towards small spindle shaped cells. Measuring bar: 100 µm.

Article Snippet: Endothelial cells: Human umbilical vein endothelial cells (HUVEC) and GFP transfected HUVEC (Pelobiotech, Martinsried, Germany) were maintained in Endothelial Cell Growth Medium (MVEM, Pelobiotech, Martinsried, Germany) containing all supplements and subcultured at 80% confluency.

Techniques: Control, Fluorescence, Comparison