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In vitro development of fresh bovine IVM oocytes (fresh control), bovine IVM oocytes treated with vitrification solution (solution control) and bovine IVM oocytes vitrified using the MVAC or Cryotop device after IVF and in vitro culture for 9 days

Journal: The Journal of Reproduction and Development

Article Title: Comparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts

doi: 10.1262/jrd.2014-163

Figure Lengend Snippet: In vitro development of fresh bovine IVM oocytes (fresh control), bovine IVM oocytes treated with vitrification solution (solution control) and bovine IVM oocytes vitrified using the MVAC or Cryotop device after IVF and in vitro culture for 9 days

Article Snippet: Thereafter, they were vitrified using either the MVAC device or the Cryotop device (Kitazato BioPharma, Shizuoka, Japan) in a vitrification solution, as described previously by Dinnyes et al . [ ].

Techniques: In Vitro

Cell numbers in blastocysts derived from fresh bovine IVM oocytes (fresh control), bovine IVM oocytes treated with vitrification solution (solution control) or bovine IVM oocytes vitrified by the MVAC or Cryotop methods after IVF and in vitro culture for 9 days. Data presented as the mean no. of nuclei ± SEM. Fresh control: IVM oocytes without any vitrification treatments. Solution control: IVM oocytes that were exposed to vitrification and warming solutions. MVAC: IVM oocytes that were vitrified by inserting the MVAC device containing them into a precooled 0.25-ml plastic straw. MVAC in LN 2 : IVM oocytes that were vitrified by plunging the MVAC device containing them directly into LN 2 . Cryotop: IVM oocytes that were vitrified by the Cryotop method. ICM: inner cell mass. TE: trophectoderm. No significant differences in ICM and TE cell numbers were detected between the treatment groups at P < 0.05 using one-way ANOVA.

Journal: The Journal of Reproduction and Development

Article Title: Comparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts

doi: 10.1262/jrd.2014-163

Figure Lengend Snippet: Cell numbers in blastocysts derived from fresh bovine IVM oocytes (fresh control), bovine IVM oocytes treated with vitrification solution (solution control) or bovine IVM oocytes vitrified by the MVAC or Cryotop methods after IVF and in vitro culture for 9 days. Data presented as the mean no. of nuclei ± SEM. Fresh control: IVM oocytes without any vitrification treatments. Solution control: IVM oocytes that were exposed to vitrification and warming solutions. MVAC: IVM oocytes that were vitrified by inserting the MVAC device containing them into a precooled 0.25-ml plastic straw. MVAC in LN 2 : IVM oocytes that were vitrified by plunging the MVAC device containing them directly into LN 2 . Cryotop: IVM oocytes that were vitrified by the Cryotop method. ICM: inner cell mass. TE: trophectoderm. No significant differences in ICM and TE cell numbers were detected between the treatment groups at P < 0.05 using one-way ANOVA.

Article Snippet: Thereafter, they were vitrified using either the MVAC device or the Cryotop device (Kitazato BioPharma, Shizuoka, Japan) in a vitrification solution, as described previously by Dinnyes et al . [ ].

Techniques: Derivative Assay, In Vitro

Development to the hatched blastocyst stage of in vitro -produced expanded blastocysts vitrified using the MVAC or Cryotop device, warmed and cultured in vitro for 72 h

Journal: The Journal of Reproduction and Development

Article Title: Comparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts

doi: 10.1262/jrd.2014-163

Figure Lengend Snippet: Development to the hatched blastocyst stage of in vitro -produced expanded blastocysts vitrified using the MVAC or Cryotop device, warmed and cultured in vitro for 72 h

Article Snippet: Thereafter, they were vitrified using either the MVAC device or the Cryotop device (Kitazato BioPharma, Shizuoka, Japan) in a vitrification solution, as described previously by Dinnyes et al . [ ].

Techniques: In Vitro, Produced, Cell Culture